Gene
Name:	hemL
Synonym(s):	ECK0153, EG10432, b0154, gsa, popC
Genome position(nucleotides):	173602 <-- 174882 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.5
External database links:

ASAP:	ABE-0000529
CGSC:	372
ECHOBASE:	EB0427
ECOLIHUB:	hemL
OU-MICROARRAY:	b0154
STRING:	511145.b0154
COLOMBOS:	hemL

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_290	173420	forward	nd	[RS-EPT-CBR]	[11]
promoter	TSS_291	173769	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_292 (cluster)	174930	forward	nd	[RS-EPT-CBR]	[11]
promoter	TSS_293 (cluster)	174942	forward	nd	[RS-EPT-CBR]	[11]
promoter	TSS_294 (cluster)	175008	forward	nd	[RS-EPT-CBR]	[11]
promoter	TSS_295	175012	forward	nd	[RS-EPT-CBR]	[11]
promoter	clcAp10	175052	forward	nd	[ICWHO]	[12]
promoter	TSS_296	175074	forward	nd	[RS-EPT-CBR]	[11]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Aiguo Z., Ruiwen D., Meizhi Z., 2019, Multi-enzymatic recycling of ATP and NADPH for the synthesis of 5-aminolevulinic acid using a semipermeable reaction system., Biosci Biotechnol Biochem 83(12):2213-2219
[2] Cui Z., Jiang Z., Zhang J., Zheng H., Jiang X., Gong K., Liang Q., Wang Q., Qi Q., 2019, Stable and Efficient Biosynthesis of 5-Aminolevulinic Acid Using Plasmid-Free Escherichia coli., J Agric Food Chem 67(5):1478-1483
[3] Eguchi Y., Okada T., Minagawa S., Oshima T., Mori H., Yamamoto K., Ishihama A., Utsumi R., 2004, Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli., J Bacteriol 186(10):3006-14
[4] Grimm B., Bull A., Breu V., 1991, Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a cyanobacterium and Escherichia coli., Mol Gen Genet 225(1):1-10
[5] Ikemi M., Murakami K., Hashimoto M., Murooka Y., 1992, Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli., Gene 121(1):127-32
[6] Kang Z., Wang Y., Gu P., Wang Q., Qi Q., 2011, Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose., Metab Eng 13(5):492-8
[7] O'Neill GP., Chen MW., Soll D., 1989, delta-Aminolevulinic acid biosynthesis in Escherichia coli and Bacillus subtilis involves formation of glutamyl-tRNA., FEMS Microbiol Lett 51(3):255-9
[8] Zhang J., Kang Z., Chen J., Du G., 2015, Optimization of the heme biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia coli., Sci Rep 5:8584
[9] Zhang J., Kang Z., Ding W., Chen J., Du G., 2016, Integrated Optimization of the In Vivo Heme Biosynthesis Pathway and the In Vitro Iron Concentration for 5-Aminolevulinate Production., Appl Biochem Biotechnol 178(6):1252-62
[10] Zhang J., Weng H., Zhou Z., Du G., Kang Z., 2019, Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli., Bioresour Technol 274:353-360
[11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[12] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Transcriptional Regulation
	Display Regulation
Activated by:	PhoP
Repressed by:	PdhR