RegulonDB

Gene
Name:	metJ
Synonym(s):	ECK3930, EG10588, b3938
Genome position(nucleotides):	4128078 <-- 4128395 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.89
External database links:

ASAP:	ABE-0012874
CGSC:	508
ECHOBASE:	EB0583
ECOLIHUB:	metJ
OU-MICROARRAY:	b3938
STRING:	511145.b3938
COLOMBOS:	metJ

Shine dalgarno
Sequence:	acgaagaggaTTAAGTatctCAT

Product

Name:

DNA-binding transcriptional repressor MetJ

Synonym(s):

MetJ

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

MetJ

Cellular location:

cytosol

Molecular weight:

12.141

Isoelectric point:

5.245

Motif(s):

Type	Positions	Sequence
	2 -> 105	AEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY
	3 -> 99	EWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGIN
	61 -> 61	A
	57 -> 57	L

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity GO:0002055 - adenine binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0006555 - methionine metabolic process GO:0008652 - cellular amino acid biosynthetic process GO:0009086 - methionine biosynthetic process GO:0045892 - negative regulation of transcription, DNA-templated

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

metJ

Operon arrangement:

Transcription unit	Promoter
metJ	metJp3
metJ	metJp2
metJ	metJp1

Transcriptional Regulation
	Display Regulation
Repressed by:	Fur

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4642	4128635	forward	nd	[RS-EPT-CBR]	[1]
promoter	TSS_4643	4129260	forward	nd	[RS-EPT-CBR]	[1]
promoter	TSS_4644	4129519	forward	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.