Gene
Name:	greB
Synonym(s):	ECK3393, EG11578, b3406
Genome position(nucleotides):	3536812 --> 3537288
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.9
External database links:

ASAP:	ABE-0011117
CGSC:	35448
ECHOBASE:	EB1538
ECOLIHUB:	greB
OU-MICROARRAY:	b3406
STRING:	511145.b3406
COLOMBOS:	greB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4001	3536371	reverse	nd	[RS-EPT-CBR]	[9]
promoter	TSS_4002	3536377	reverse	nd	[RS-EPT-CBR]	[9]
promoter	greBp8	3536744	forward	nd	[COMP-AINF]	[10]
promoter	greBp7	3536780	forward	nd	[COMP-AINF]	[10]
promoter	yhgFp2	3537176	forward	nd	[COMP-AINF]	[10]
promoter	TSS_4004	3537268	forward	nd	[RS-EPT-CBR]	[9]
promoter	TSS_4005 (cluster)	3537353	forward	nd	[RS-EPT-CBR]	[9]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Altmann CR., Solow-Cordero DE., Chamberlin MJ., 1994, RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme.RNA complex., Proc Natl Acad Sci U S A 91(9):3784-8
[2] Borukhov S., Goldfarb A., 1996, Purification and assay of Escherichia coli transcript cleavage factors GreA and GreB., Methods Enzymol 274:315-26
[3] Hung SC., Gottesman ME., 1997, The Nun protein of bacteriophage HK022 inhibits translocation of Escherichia coli RNA polymerase without abolishing its catalytic activities., Genes Dev 11(20):2670-8
[4] Koulich D., Nikiforov V., Borukhov S., 1998, Distinct functions of N and C-terminal domains of GreA, an Escherichia coli transcript cleavage factor., J Mol Biol 276(2):379-89
[5] Orlova M., Newlands J., Das A., Goldfarb A., Borukhov S., 1995, Intrinsic transcript cleavage activity of RNA polymerase., Proc Natl Acad Sci U S A 92(10):4596-600
[6] Trautinger BW., Lloyd RG., 2002, Modulation of DNA repair by mutations flanking the DNA channel through RNA polymerase., EMBO J 21(24):6944-53
[7] Wang D., Landick R., 1997, Nuclease cleavage of the upstream half of the nontemplate strand DNA in an Escherichia coli transcription elongation complex causes upstream translocation and transcriptional arrest., J Biol Chem 272(9):5989-94
[8] Zakharova N., Bass I., Arsenieva E., Nikiforov V., Severinov K., 1998, Mutations in and monoclonal antibody binding to evolutionary hypervariable region of Escherichia coli RNA polymerase beta' subunit inhibit transcript cleavage and transcript elongation., J Biol Chem 273(38):24912-20
[9] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[10] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78