RegulonDB RegulonDB 11.1: Gene Form
   

moaE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ybhL moaE moaD moaC YjjQ TSS_952 TSS_952 TSS_951 (cluster) TSS_951 (cluster) TSS_950 TSS_950 ybhLp ybhLp

Gene      
Name: moaE    Texpresso search in the literature
Synonym(s): ECK0774, EG11598, b0785, chlA5
Genome position(nucleotides): 819295 --> 819747
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.73
External database links:  
ASAP:
 ABE-0002678
CGSC:
 31228
ECHOBASE:
 EB1555
ECOLIHUB:
 moaE
MIM:
 252160
OU-MICROARRAY:
 b0785
STRING:
 511145.b0785
COLOMBOS: moaE


Product      
Name: molybdopterin synthase catalytic subunit
Synonym(s): MoaE, molybdopterin synthase large subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 16.981
Isoelectric point: 5.054
Motif(s):
 
Type Positions Sequence Comment
2 -> 150 AETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLGDSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW UniProt: Molybdopterin synthase catalytic subunit.
11 -> 121 QPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLGDSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKTR
34 -> 34 F UniProt: 4-fold lower activity than wild-type..
37 -> 39 KVR UniProt: Substrate binding; Sequence Annotation Type: region of interest.
39 -> 39 R UniProt: 24-fold lower activity than wild-type..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.4 - molybdenum
Gene Ontology Terms (GO)  
cellular_component GO:1990140 - MPT synthase complex
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0042803 - protein homodimerization activity
GO:0030366 - molybdopterin synthase activity
biological_process GO:0006777 - Mo-molybdopterin cofactor biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P30749
DIP:
 DIP-10232N
ECOCYC:
 EG11598-MONOMER
ECOLIWIKI:
 b0785
INTERPRO:
 IPR036563
INTERPRO:
 IPR003448
MODBASE:
 P30749
PDB:
 3BII
PDB:
 1NVJ
PDB:
 1NVI
PDB:
 1FM0
PDB:
 1FMA
PFAM:
 PF02391
PRIDE:
 P30749
REFSEQ:
 NP_415306
SMR:
 P30749
UNIPROT:
 P30749


Operon      
Name: moaABCDE         
Operon arrangement:
Transcription unit        Promoter
moaABCDE
moaABCDE
moaBCDE


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, ModE
Repressed by: CueR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_950 819869 forward nd [RS-EPT-CBR] [1]
  promoter TSS_951 (cluster) 819906 forward nd [RS-EPT-CBR] [1]
  promoter TSS_952 819916 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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