RegulonDB RegulonDB 11.1: Gene Form
   

nuoH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nuoG nuoF nuoH nuoI nuoE Cis-reg TSS_2551 TSS_2551 TSS_2550 TSS_2550 TSS_2549 TSS_2549 TSS_2548 TSS_2548 TSS_2547 TSS_2547 TSS_2546 TSS_2546 TSS_2545 TSS_2545 TSS_2544 TSS_2544 TSS_2543 TSS_2543 TSS_2542 TSS_2542 TSS_2541 TSS_2541 TSS_2540 TSS_2540

Gene      
Name: nuoH    Texpresso search in the literature
Synonym(s): ECK2276, EG12088, b2282
Genome position(nucleotides): 2396465 <-- 2397442
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.19
External database links:  
ASAP:
 ABE-0007541
CGSC:
 32658
ECHOBASE:
 EB2012
ECOLIHUB:
 nuoH
MIM:
 540000
MIM:
 535000
MIM:
 252010
MIM:
 125853
MIM:
 502500
OU-MICROARRAY:
 b2282
STRING:
 511145.b2282
COLOMBOS: nuoH


Product      
Name: NADH:quinone oxidoreductase subunit H
Synonym(s): NADH:ubiquinone oxidoreductase, membrane subunit H, NuoH
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 36.219
Isoelectric point: 7.497
Motif(s):
 
Type Positions Sequence Comment
11 -> 31 ILLTILKAVVILLVVVTCGAF UniProt: Helical.
17 -> 317 KAVVILLVVVTCGAFMSFGERRLLGLFQNRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVPVSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFLGLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTAFFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTA
35 -> 36 GE UniProt: In Ref. 1; CAA48367..
53 -> 53 G UniProt: In Ref. 1; CAA48367..
81 -> 101 VIFTLAPMIAFTSLLLAFAIV UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.D - Oxidoreduction-driven Active Transporters
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0030964 - NADH dehydrogenase complex
GO:0045271 - respiratory chain complex I
GO:0045272 - plasma membrane respiratory chain complex I
molecular_function GO:0016655 - oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
GO:0003954 - NADH dehydrogenase activity
GO:0008137 - NADH dehydrogenase (ubiquinone) activity
GO:0048038 - quinone binding
biological_process GO:0009060 - aerobic respiration
GO:0022904 - respiratory electron transport chain
Note(s): Note(s): ...[more].
Reference(s): [1] Gustavsson T., et al., 2010
[2] Jahn LJ., et al., 2017
[3] Li B., et al., 2012
[4] Novakovsky GE., et al., 2016
[5] Sinha PK., et al., 2015
External database links:  
ALPHAFOLD:
 P0AFD4
ECOCYC:
 NUOH-MONOMER
ECOLIWIKI:
 b2282
INTERPRO:
 IPR001694
INTERPRO:
 IPR018086
PANTHER:
 PTHR11432
PFAM:
 PF00146
PRIDE:
 P0AFD4
PRODB:
 PRO_000023435
PROSITE:
 PS00668
PROSITE:
 PS00667
REFSEQ:
 NP_416785
UNIPROT:
 P0AFD4


Operon      
Name: nuoABCEFGHIJKLMN         
Operon arrangement:
Transcription unit        Promoter
nuoABCEFGHIJKLMN
nuoABCEFGHIJKLMN
nuoMN


Transcriptional Regulation      
Display Regulation             
Activated by: NarL, Fis
Repressed by: FNR, IHF, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2540 2396044 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2541 2396059 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2542 2396436 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2543 2396485 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2544 2396590 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2545 2396594 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2546 2396596 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2547 2397023 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2548 2397784 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2549 2398792 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2550 2399056 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2551 2401472 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gustavsson T., Trane M., Moparthi VK., Miklovyte E., Moparthi L., Gorecki K., Leiding T., Arskold SP., Hagerhall C., 2010, A cytochrome c fusion protein domain for convenient detection, quantification, and enhanced production of membrane proteins in Escherichia coli--expression and characterization of cytochrome-tagged Complex I subunits., Protein Sci 19(8):1445-60

 [2] Jahn LJ., Munck C., Ellabaan MMH., Sommer MOA., 2017, Adaptive Laboratory Evolution of Antibiotic Resistance Using Different Selection Regimes Lead to Similar Phenotypes and Genotypes., Front Microbiol 8:816

 [3] Li B., Vik SB., Tu Y., 2012, Theaflavins inhibit the ATP synthase and the respiratory chain without increasing superoxide production., J Nutr Biochem 23(8):953-60

 [4] Novakovsky GE., Dibrova DV., Mulkidjanian AY., 2016, Phylogenomic Analysis of Type 1 NADH:Quinone Oxidoreductase., Biochemistry (Mosc) 81(7):770-84

 [5] Sinha PK., Castro-Guerrero N., Patki G., Sato M., Torres-Bacete J., Sinha S., Miyoshi H., Matsuno-Yagi A., Yagi T., 2015, Conserved amino acid residues of the NuoD segment important for structure and function of Escherichia coli NDH-1 (complex I)., Biochemistry 54(3):753-64

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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