RegulonDB

Gene
Name:	csgG
Synonym(s):	ECK1023, G6543, b1037
Genome position(nucleotides):	1100851 <-- 1101684 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.12
External database links:

ASAP:	ABE-0003516
ECHOBASE:	EB3189
ECOLIHUB:	csgG
OU-MICROARRAY:	b1037
STRING:	511145.b1037
COLOMBOS:	csgG

Gene

Name:

csgG

Synonym(s):

ECK1023, G6543, b1037

Genome position(nucleotides):

1100851 <-- 1101684 Genome Browser

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

50.12

External database links:

ASAP:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Product

Name:

curli secretion channel

Synonym(s):

CsgG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

periplasmic space,inner membrane,outer membrane

Molecular weight:

30.557

Isoelectric point:

7.41

Motif(s):

Type	Positions	Sequence
	34 -> 41	QSYKDLTH
	41 -> 255	HLPAPTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQNLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFGIGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQ
	49 -> 58	IFVSVYNIQD
	213 -> 222	RLLEGEVGYT
	216 -> 224	EGEVGYTSN

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		4 - transport --> 4.1 - Channel-type Transporters
Gene Ontology Terms (GO)
	cellular_component	GO:0062155 - curli secretion complex GO:0016020 - membrane GO:0030288 - outer membrane-bounded periplasmic space GO:0005886 - plasma membrane GO:0045203 - integral component of cell outer membrane
	molecular_function	GO:0005515 - protein binding GO:0042802 - identical protein binding
	biological_process	GO:0098775 - curli assembly GO:0098777 - protein secretion by the type VIII secretion system

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Gonnet P., et al., 2004
[2] Hammar M., et al., 1995
[3] Juncker AS., et al., 2003
[4] Perry TN., et al., 2019
[5] Rao S., et al., 2020
[6] Robinson LS., et al., 2006

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PDB:

PDB:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, OmpR, IHF, RcdA, Cra, BasR, CsgD, BolA, MlrA
Repressed by:	FliZ, MqsA, H-NS, CpxR, RcsAB, RstA, BtsR

Transcriptional Regulation

Display Regulation

Activated by:

CRP, OmpR, IHF, RcdA, Cra, BasR, CsgD, BolA, MlrA

Repressed by:

FliZ, MqsA, H-NS, CpxR, RcsAB, RstA, BtsR

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	csgGp1	1101755		reverse	nd	[ICWHO]	[7]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

csgGp1

1101755

reverse

[ICWHO]

[7]

Evidence
[ICWHO] Inferred computationally without human oversight

Evidence

[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Gonnet P., Rudd KE., Lisacek F., 2004, Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12., Proteomics 4(6):1597-613
[2] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70
[3] Juncker AS., Willenbrock H., Von Heijne G., Brunak S., Nielsen H., Krogh A., 2003, Prediction of lipoprotein signal peptides in Gram-negative bacteria., Protein Sci 12(8):1652-62
[4] Perry TN., Souabni H., Rapisarda C., Fronzes R., Giusti F., Popot JL., Zoonens M., Gubellini F., 2019, BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy., Biochim Biophys Acta Biomembr 1861(2):466-477
[5] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3
[6] Robinson LS., Ashman EM., Hultgren SJ., Chapman MR., 2006, Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein., Mol Microbiol 59(3):870-81
[7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Gonnet P., Rudd KE., Lisacek F., 2004, Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12., Proteomics 4(6):1597-613

[2] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70

[3] Juncker AS., Willenbrock H., Von Heijne G., Brunak S., Nielsen H., Krogh A., 2003, Prediction of lipoprotein signal peptides in Gram-negative bacteria., Protein Sci 12(8):1652-62

[4] Perry TN., Souabni H., Rapisarda C., Fronzes R., Giusti F., Popot JL., Zoonens M., Gubellini F., 2019, BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy., Biochim Biophys Acta Biomembr 1861(2):466-477

[5] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

[6] Robinson LS., Ashman EM., Hultgren SJ., Chapman MR., 2006, Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein., Mol Microbiol 59(3):870-81

[7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78