RegulonDB

Gene
Name:	mcbR
Synonym(s):	ECK1444, G6761, b1450, yncC
Genome position(nucleotides):	1520262 --> 1520927 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	43.69
External database links:

ASAP:	ABE-0004837
ECHOBASE:	EB3535
OU-MICROARRAY:	b1450
PortEco:	mcbR
STRING:	511145.b1450
COLOMBOS:	mcbR

Product

Name:

DNA-binding transcriptional dual regulator McbR

Synonym(s):

McbR, YncC

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

GntR

Cellular location:

cytosol

Molecular weight:

25.151

Isoelectric point:

6.883

Motif(s):

Type	Positions	Sequence
	10 -> 77	VSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEV
	13 -> 74	TLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTV
	37 -> 56	TKNLAEQLGMSITPVREALL
	85 -> 209	INRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQ

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0045892 - negative regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[AIFS] Automated inference of function from sequence
[APPHH] Assay of protein partially-purified from a heterologous host
[AUP] Assay of unpurified protein
[IEP] Inferred from expression pattern

Reference(s):

[1] Beraud M., et al., 2010
[2] Perez-Rueda E., et al., 2004
[3] Zhang XS., et al., 2008

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMART:

SMART:

SMR:

UNIPROT:

Operon

Name:

mcbR

Operon arrangement:

Transcription unit	Promoter

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	mcbRp3	1520096		forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 7.205. P-value: 5.672e-6 Read more >	[ICWHO]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Beraud M., Kolb A., Monteil V., D'Alayer J., Norel F., 2010, A proteomic analysis reveals differential regulation of the σ(S)-dependent yciGFE(katN) locus by YncC and H-NS in Salmonella and Escherichia coli K-12., Mol Cell Proteomics 9(12):2601-16
[2] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50
[3] Zhang XS., Garcia-Contreras R., Wood TK., 2008, Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)., ISME J 2(6):615-31
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78