RegulonDB RegulonDB 11.1: Gene Form
   

alaA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

alaA lrhA yfbR anti-anti-terminator anti-terminator terminator terminator terminator yfbRp4 yfbRp4 yfbRp3 yfbRp3 alaAp7 alaAp7 alaAp9 alaAp9 TSS_2569 TSS_2569 lrhAp lrhAp TSS_2568 TSS_2568 TSS_2567 (cluster) TSS_2567 (cluster) TSS_2566 (cluster) TSS_2566 (cluster)

Gene      
Name: alaA    Texpresso search in the literature
Synonym(s): ECK2284, G7184, b2290, yfbQ
Genome position(nucleotides): 2407561 --> 2408778
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.49
External database links:  
ASAP:
 ABE-0007560
ECHOBASE:
 EB3854
ECOLIHUB:
 yfbQ
MIM:
 276600
OU-MICROARRAY:
 b2290
STRING:
 511145.b2290
COLOMBOS: alaA


Product      
Name: glutamate—pyruvate aminotransferase AlaA
Synonym(s): AlaA, YfbQ
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 45.517
Isoelectric point: 6.186
Motif(s):
 
Type Positions Sequence Comment
58 -> 387 VIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDD

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.17 - alanine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0008483 - transaminase activity
GO:0030170 - pyridoxal phosphate binding
GO:0004021 - L-alanine:2-oxoglutarate aminotransferase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0006523 - alanine biosynthetic process
GO:0006974 - cellular response to DNA damage stimulus
GO:0008652 - cellular amino acid biosynthetic process
GO:0009058 - biosynthetic process
GO:0046677 - response to antibiotic
GO:0019272 - L-alanine biosynthetic process from pyruvate
GO:0030632 - D-alanine biosynthetic process
External database links:  
ALPHAFOLD:
 P0A959
DIP:
 DIP-11970N
ECOCYC:
 G7184-MONOMER
ECOLIWIKI:
 b2290
INTERPRO:
 IPR015422
INTERPRO:
 IPR015424
INTERPRO:
 IPR015421
INTERPRO:
 IPR004839
MODBASE:
 P0A959
PDB:
 4CVQ
PFAM:
 PF00155
PRIDE:
 P0A959
REFSEQ:
 NP_416793
SMR:
 P0A959
SWISSMODEL:
 P0A959
UNIPROT:
 P0A959


Operon      
Name: alaA-yfbR         
Operon arrangement:
Transcription unit        Promoter
alaA
yfbR
alaA-yfbR


Transcriptional Regulation      
Display Regulation             
Repressed by: Lrp


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2566 (cluster) 2405828 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2567 (cluster) 2406635 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2568 2406641 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2569 2407012 reverse nd [RS-EPT-CBR] [1]
  promoter alaAp7 2407414 forward nd [COMP-AINF] [2]
  promoter yfbRp4 2408835 forward nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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