RegulonDB RegulonDB 11.1: Gene Form
   

cydC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cydD cydC aat TSS_1058 TSS_1058 cydCp5 cydCp5 TSS_1057 TSS_1057 aatp5 aatp5

Gene      
Name: cydC    Texpresso search in the literature
Synonym(s): ECK0877, EG10012, b0886, mdrA, mdrH, surB, ycaB
Genome position(nucleotides): 927474 <-- 929195
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.77
External database links:  
ASAP:
 ABE-0003012
CGSC:
 17725
ECHOBASE:
 EB0012
ECOLIHUB:
 cydC
OU-MICROARRAY:
 b0886
STRING:
 511145.b0886
COLOMBOS: cydC


Product      
Name: glutathione/L-cysteine ABC exporter subunit CydC
Synonym(s): CydC, L-cystine reductase subunit CydC, MdrA, MdrH, SurB, YcaB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 62.92
Isoelectric point: 8.902
Motif(s):
 
Type Positions Sequence Comment
16 -> 36 WMLSLGIVLAIVTLLASIGLL UniProt: Helical.
18 -> 306 LSLGIVLAIVTLLASIGLLTLSGWFLSASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSKLLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDFTLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGASDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQPGALIALFVFCALAAFEALAPVTGAFQHLGQ UniProt: ABC transmembrane type-1.
37 -> 46 TLSGWFLSAS small periplasmic loop
47 -> 67 AVAGVAGLYSFNYMLPAAGVR
58 -> 287 NYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSKLLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDFTLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGASDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQPGALIALFVFCA

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.am - ABC superfamily, ATP binding and membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
molecular_function GO:0140359 - ABC-type transporter activity
GO:0016887 - ATP hydrolysis activity
GO:0042626 - ATPase-coupled transmembrane transporter activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
biological_process GO:0045454 - cell redox homeostasis
GO:0055085 - transmembrane transport
GO:0033228 - cysteine export across plasma membrane
GO:0034775 - glutathione transmembrane transport
GO:0070453 - regulation of heme biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Mironov A., et al., 2020
[2] Xu Y., et al., 2017
External database links:  
ALPHAFOLD:
 P23886
DIP:
 DIP-9362N
ECOCYC:
 CYDC-MONOMER
ECOLIWIKI:
 b0886
INTERPRO:
 IPR027417
INTERPRO:
 IPR036640
INTERPRO:
 IPR017871
INTERPRO:
 IPR014223
INTERPRO:
 IPR003593
INTERPRO:
 IPR003439
INTERPRO:
 IPR011527
MODBASE:
 P23886
PFAM:
 PF00664
PFAM:
 PF00005
PRIDE:
 P23886
PRODB:
 PRO_000022363
PROSITE:
 PS00211
PROSITE:
 PS50929
PROSITE:
 PS50893
REFSEQ:
 NP_415406
SMART:
 SM00382
SMR:
 P23886
UNIPROT:
 P23886


Operon      
Name: cydDC         
Operon arrangement:
Transcription unit        Promoter
cydDC


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, NarL, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter aatp5 927460 reverse nd [COMP-AINF] [3]
  promoter TSS_1057 928505 reverse nd [RS-EPT-CBR] [4]
  promoter cydCp5 929400 reverse nd [COMP-AINF] [3]
  promoter TSS_1058 929741 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Mironov A., Seregina T., Shatalin K., Nagornykh M., Shakulov R., Nudler E., 2020, CydDC functions as a cytoplasmic cystine reductase to sensitize Escherichia coli to oxidative stress and aminoglycosides., Proc Natl Acad Sci U S A 117(38):23565-23570

 [2] Xu Y., Liu Y., Li F., Cao G., Zheng P., Sun J., Wen J., Zhang D., 2017, Identification of a new gene yecC involved in threonine export in Escherichia coli., FEMS Microbiol Lett 364(17)

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB