RegulonDB RegulonDB 11.0: Gene Form
   

acrD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

acrD narQ ypfM BaeR CpxR CpxR EvgA CpxR acrDp2 acrDp2 acrDp acrDp

Gene      
Name: acrD    Texpresso search in the literature
Synonym(s): ECK2465, EG10014, b2470, yffA
Genome position(nucleotides): 2587595 --> 2590708
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.46
External database links:  
ASAP:
 ABE-0008136
CGSC:
 35697
ECHOBASE:
 EB0014
ECOLIHUB:
 acrD
OU-MICROARRAY:
 b2470
STRING:
 511145.b2470
COLOMBOS: acrD


Product      
Name: multidrug efflux pump RND permease AcrD
Synonym(s): AcrD, YffA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 113.047
Isoelectric point: 5.614
Motif(s):
 
Type Positions Sequence Comment
1 -> 1027 MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVRVTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDEAVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVASNIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQLGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYDYLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVKASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNTLTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQKGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLEDRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVARMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDMELQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVAIDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVPFSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLEWTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPILMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVR
10 -> 28 IFAWVLAILLCLTGTLAIF UniProt: Helical; Name=1.
303 -> 303 A UniProt: In Ref. 1; AAA20584..
340 -> 359 VVKTLLEAIALVFLVMYLFL UniProt: Helical; Name=2.
366 -> 385 LIPTIAVPVVLMGTFSVLYA UniProt: Helical; Name=3.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0098567 - periplasmic side of plasma membrane
GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:1990281 - efflux pump complex
molecular_function GO:0015125 - bile acid transmembrane transporter activity
GO:0005515 - protein binding
GO:0015562 - efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
GO:0042910 - xenobiotic transmembrane transporter activity
biological_process GO:0140330 - xenobiotic detoxification by transmembrane export across the cell outer membrane
GO:0009636 - response to toxic substance
GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0015721 - bile acid and bile salt transport
Note(s): Note(s): ...[more].
Reference(s): [1] Alon Cudkowicz N., et al., 2019
[2] Bleuel C., et al., 2005
[3] Elkins CA., et al., 2006
[4] Jindal S., et al., 2019
[5] Pu Y., et al., 2016
[6] Shuster Y., et al., 2016
[7] Will J., et al., 2008
External database links:  
ALPHAFOLD:
 P24177
DIP:
 DIP-9050N
ECOCYC:
 ACRD-MONOMER
ECOLIWIKI:
 b2470
INTERPRO:
 IPR004764
INTERPRO:
 IPR027463
INTERPRO:
 IPR001036
MODBASE:
 P24177
PANTHER:
 PTHR32063
PFAM:
 PF00873
PRIDE:
 P24177
PRINTS:
 PR00702
REFSEQ:
 NP_416965
SMR:
 P24177
SWISSMODEL:
 P24177
UNIPROT:
 P24177


Operon      
Name: acrD         
Operon arrangement:
Transcription unit        Promoter
acrD
acrD


Transcriptional Regulation      
Display Regulation             
Activated by: EvgA, BaeR, CpxR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828

 [2] Bleuel C., Grosse C., Taudte N., Scherer J., Wesenberg D., Krauss GJ., Nies DH., Grass G., 2005, TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli., J Bacteriol 187(19):6701-7

 [3] Elkins CA., Mullis LB., 2006, Mammalian steroid hormones are substrates for the major RND- and MFS-type tripartite multidrug efflux pumps of Escherichia coli., J Bacteriol 188(3):1191-5

 [4] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [5] Pu Y., Zhao Z., Li Y., Zou J., Ma Q., Zhao Y., Ke Y., Zhu Y., Chen H., Baker MAB., Ge H., Sun Y., Xie XS., Bai F., 2016, Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells., Mol Cell 62(2):284-294

 [6] Shuster Y., Steiner-Mordoch S., Alon Cudkowicz N., Schuldiner S., 2016, A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics., PLoS One 11(4):e0152917

 [7] Will J., Sheldrick WS., Wolters D., 2008, Characterisation of cisplatin coordination sites in cellular Escherichia coli DNA-binding proteins by combined biphasic liquid chromatography and ESI tandem mass spectrometry., J Biol Inorg Chem 13(3):421-34

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