RegulonDB RegulonDB 10.8: Gene Form
   

ackA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pta ackA yfbV anti-anti-terminator terminator anti-terminator TSS_2612 TSS_2612 TSS_2611 TSS_2611 TSS_2610 TSS_2610 TSS_2609 TSS_2609 TSS_2608 TSS_2608 TSS_2607 TSS_2607 TSS_2606 (cluster) TSS_2606 (cluster) TSS_2605 TSS_2605 TSS_2604 TSS_2604 ptap ptap TSS_2602 TSS_2602 TSS_2601 TSS_2601 TSS_2600 TSS_2600 TSS_2599 TSS_2599 TSS_2598 TSS_2598 TSS_2597 TSS_2597 TSS_2596 TSS_2596 TSS_2595 TSS_2595 TSS_2594 TSS_2594 TSS_2593 TSS_2593 TSS_2592 (cluster) TSS_2592 (cluster) ackAp ackAp TSS_2591 (cluster) TSS_2591 (cluster) TSS_2590 (cluster) TSS_2590 (cluster) TSS_2589 (cluster) TSS_2589 (cluster) TSS_2588 (cluster) TSS_2588 (cluster) TSS_2587 (cluster) TSS_2587 (cluster) TSS_2586 TSS_2586 TSS_2585 TSS_2585

Gene      
Name: ackA    Texpresso search in the literature
Synonym(s): ECK2290, EG10027, b2296
Genome position(nucleotides): 2413470 --> 2414672 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.2
Reference(s): [1] Matsuyama A., et al., 1989
External database links:  
ASAP:
 ABE-0007579
CGSC:
 1048
ECHOBASE:
 EB0026
OU-MICROARRAY:
 b2296
PortEco:
 ackA
STRING:
 511145.b2296
COLOMBOS: ackA


Product      
Name: acetate kinase
Synonym(s): AckA, acetate kinase A, propionate kinase 2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 43.29
Isoelectric point: 6.237
Motif(s):
 
Type Positions Sequence
221 -> 221 R
240 -> 240 M
285 -> 287 DCR
6 -> 393 VLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQ
210 -> 214 HLGNG

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.28 - pyruvate oxidation
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.29 - acetate catabolism
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.30 - threonine catabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0016774 - phosphotransferase activity, carboxyl group as acceptor
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0008776 - acetate kinase activity
GO:0000287 - magnesium ion binding
biological_process GO:0006082 - organic acid metabolic process
GO:0006083 - acetate metabolic process
GO:0006085 - acetyl-CoA biosynthetic process
GO:0016310 - phosphorylation
GO:0044011 - single-species biofilm formation on inanimate substrate
GO:0019413 - acetate biosynthetic process
GO:0019542 - propionate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-9042N
ECOCYC:
 ACETATEKINA-MONOMER
ECOLIWIKI:
 b2296
INTERPRO:
 IPR023865
INTERPRO:
 IPR004372
INTERPRO:
 IPR000890
MODBASE:
 P0A6A3
PANTHER:
 PTHR21060
PDB:
 1LRG
PFAM:
 PF00871
PRIDE:
 P0A6A3
PRINTS:
 PR00471
PRODB:
 PRO_000022041
PROSITE:
 PS01075
PROSITE:
 PS01076
REFSEQ:
 NP_416799
SMR:
 P0A6A3
UNIPROT:
 P0A6A3


Operon      
Name: ackA-pta         
Operon arrangement:
Transcription unit        Promoter
ackA
pta
ackA-pta


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2585 2412788 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2586 2413305 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2587 (cluster) 2413344 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2588 (cluster) 2413425 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2589 (cluster) 2413437 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2590 (cluster) 2413451 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2591 (cluster) 2413455 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2592 (cluster) 2413462 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2593 2413470 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2594 2413474 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2595 2413477 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2596 2413482 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2597 2413792 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2598 2413797 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2599 2413805 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2600 2413957 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2601 2414269 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2602 2414360 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2604 2415098 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2605 2415351 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2606 (cluster) 2415354 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_2607 2415750 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2608 2415781 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2609 2415814 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2610 2415818 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2611 2416038 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2612 2416149 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Matsuyama A., Yamamoto H., Nakano E., 1989, Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene., J Bacteriol 171(1):577-80

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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