RegulonDB RegulonDB 10.8: Gene Form
   

adhE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

adhE insZ tdk ychT Lrp Fis Fis Lrp Fis Lrp Cra FNR NarL NarL terminator anti-terminator anti-anti-terminator ychEp8 ychEp8 adhEp1 adhEp1 adhEp2 adhEp2 TSS_1621 TSS_1621 TSS_1620 (cluster) TSS_1620 (cluster) TSS_1619 (cluster) TSS_1619 (cluster) TSS_1618 TSS_1618 TSS_1617 TSS_1617 TSS_1616 TSS_1616 TSS_1615 TSS_1615 TSS_1614 (cluster) TSS_1614 (cluster) TSS_1613 TSS_1613 TSS_1612 TSS_1612 TSS_1611 TSS_1611 TSS_1610 TSS_1610 TSS_1609 (cluster) TSS_1609 (cluster) TSS_1608 TSS_1608 TSS_1607 TSS_1607 TSS_1606 TSS_1606 TSS_1605 TSS_1605 TSS_1604 TSS_1604 TSS_1603 TSS_1603 TSS_1602 TSS_1602 TSS_1601 (cluster) TSS_1601 (cluster) TSS_1600 TSS_1600 TSS_1599 (cluster) TSS_1599 (cluster) TSS_1598 TSS_1598 TSS_1597 TSS_1597 TSS_1596 TSS_1596 TSS_1595 TSS_1595 TSS_1594 TSS_1594 TSS_1593 TSS_1593 TSS_1592 TSS_1592 TSS_1591 TSS_1591 TSS_1590 TSS_1590 TSS_1589 TSS_1589 TSS_1588 TSS_1588 TSS_1587 TSS_1587 TSS_1586 TSS_1586 TSS_1585 TSS_1585 TSS_1584 TSS_1584 TSS_1583 TSS_1583 TSS_1582 TSS_1582 TSS_1581 TSS_1581 TSS_1580 TSS_1580 TSS_1579 TSS_1579 TSS_1578 TSS_1578 TSS_1577 TSS_1577

Gene      
Name: adhE    Texpresso search in the literature
Synonym(s): ECK1235, EG10031, adhC, ana, b1241
Genome position(nucleotides): 1295446 <-- 1298121 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.64
Reference(s): [1] Chen YM., et al., 1991
[2] Goodlove PE., et al., 1989
[3] Leonardo MR., et al., 1993
[4] Membrillo-Hernandez J., et al., 1999
[5] Membrillo-Hernandez J., et al., 1999
External database links:  
ASAP:
 ABE-0004164
CGSC:
 1041
ECHOBASE:
 EB0030
OU-MICROARRAY:
 b1241
PortEco:
 adhE
STRING:
 511145.b1241
COLOMBOS: adhE


Product      
Name: aldehyde/alcohol dehydrogenase AdhE
Synonym(s): AdhC, AdhE, Ana
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 96.127
Isoelectric point: 6.766
Motif(s):
 
Type Positions Sequence
5 -> 400 NVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAADARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAEPIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAAGAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQGKELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAHEKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTARILINTPA
422 -> 427 GSWGGN
457 -> 850 PKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGVETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHFEELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLADYALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLLKEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLANALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPL
2 -> 891 AVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAADARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAEPIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAAGAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQGKELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAHEKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTARILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGVETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHFEELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLADYALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLLKEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLANALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0016620 - oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0004022 - alcohol dehydrogenase (NAD+) activity
GO:0008774 - acetaldehyde dehydrogenase (acetylating) activity
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0006066 - alcohol metabolic process
GO:0015976 - carbon utilization
GO:0055114 - oxidation-reduction process
GO:0006115 - ethanol biosynthetic process
External database links:  
DIP:
 DIP-35790N
ECOCYC:
 ADHE-MONOMER
ECOLIWIKI:
 b1241
INTERPRO:
 IPR016161
INTERPRO:
 IPR039697
INTERPRO:
 IPR034789
INTERPRO:
 IPR018211
INTERPRO:
 IPR016163
INTERPRO:
 IPR016162
INTERPRO:
 IPR015590
INTERPRO:
 IPR012079
INTERPRO:
 IPR001670
MINT:
 P0A9Q7
MODBASE:
 P0A9Q7
PANTHER:
 PTHR11496
PDB:
 6AHC
PFAM:
 PF00171
PFAM:
 PF00465
PRIDE:
 P0A9Q7
PRODB:
 PRO_000022056
PROSITE:
 PS00060
PROSITE:
 PS00913
REFSEQ:
 NP_415757
SMR:
 P0A9Q7
UNIPROT:
 P0A9Q7


Operon      
Name: adhE         
Operon arrangement:
Transcription unit        Promoter
adhE
adhE


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, Fis
Repressed by: Cra, Lrp, NarL


Regulation by small RNA    
  Display Regulation
small RNA gcvB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1577 1295543 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1578 1295679 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1579 1295919 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1580 1296310 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1581 1296316 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1582 1296554 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1583 1296583 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1584 1296586 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1585 1296589 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1586 1296611 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1587 1296616 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1588 1296672 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1589 1296674 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1590 1296756 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1591 1296775 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1592 1296797 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1593 1296870 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1594 1296892 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1595 1296896 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1596 1297024 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1597 1297170 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1598 1297212 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1599 (cluster) 1297288 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1600 1297462 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1601 (cluster) 1297466 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1602 1297601 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1603 1297609 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1604 1297628 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1605 1297669 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1606 1297682 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1607 1297691 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1608 1297717 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1609 (cluster) 1297803 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1610 1297806 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1611 1297812 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1612 1297840 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1613 1297873 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1614 (cluster) 1297877 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1615 1298092 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1616 1298104 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1617 1298113 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1618 1298138 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1619 (cluster) 1298145 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1620 (cluster) 1298151 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_1621 1298217 reverse nd [RS-EPT-CBR] [6]
  promoter ychEp8 1298452 forward Similarity to the consensus
Read more > 		[ICWHO] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Chen YM., Lin EC., 1991, Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli., J Bacteriol 173(24):8009-13

 [2] Goodlove PE., Cunningham PR., Parker J., Clark DP., 1989, Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli., Gene 85(1):209-14

 [3] Leonardo MR., Cunningham PR., Clark DP., 1993, Anaerobic regulation of the adhE gene, encoding the fermentative alcohol dehydrogenase of Escherichia coli., J Bacteriol 175(3):870-8

 [4] Membrillo-Hernandez J., Kwon O., De Wulf P., Finkel SE., Lin EC., 1999, Regulation of adhE (encoding ethanol oxidoreductase) by the Fis protein in Escherichia coli., J Bacteriol 181(23):7390-3

 [5] Membrillo-Hernandez J., Lin EC., 1999, Regulation of expression of the adhE gene, encoding ethanol oxidoreductase in Escherichia coli: transcription from a downstream promoter and regulation by fnr and RpoS., J Bacteriol 181(24):7571-9

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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