RegulonDB RegulonDB 10.8: Gene Form
   

alaS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

alaS recX csrA AlaS alaSp alaSp TSS_3011 TSS_3011 TSS_3010 TSS_3010 TSS_3009 TSS_3009 TSS_3008 TSS_3008 TSS_3007 (cluster) TSS_3007 (cluster) TSS_3006 TSS_3006 csrAp1 csrAp1 csrAp2 csrAp2 csrAp3 csrAp3 csrAp4 csrAp4 csrAp5 csrAp5

Gene      
Name: alaS    Texpresso search in the literature
Synonym(s): ECK2692, EG10034, act, ala-act, b2697, lovB
Genome position(nucleotides): 2819381 <-- 2822011 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.63
External database links:  
ASAP:
 ABE-0008869
CGSC:
 1039
ECHOBASE:
 EB0033
MIM:
 613287
MIM:
 616339
OU-MICROARRAY:
 b2697
PortEco:
 alaS
STRING:
 511145.b2697
COLOMBOS: alaS


Product      
Name: alanine—tRNA ligase/DNA-binding transcriptional repressor
Synonym(s): Act, AlaS, LovB, ala-act
Sequence: Get amino acid sequence Fasta Format
Regulator Family: Class II aminoacyl_tRNA synthetase
Cellular location: cytosol
Molecular weight: 96.032
Isoelectric point: 5.575
Motif(s):
 
Type Positions Sequence
175 -> 175 D
182 -> 182 C
191 -> 191 D
238 -> 238 G
564 -> 564 H

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003676 - nucleic acid binding
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0004813 - alanine-tRNA ligase activity
GO:0003723 - RNA binding
GO:0000049 - tRNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0016597 - amino acid binding
GO:0008270 - zinc ion binding
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
GO:0002161 - aminoacyl-tRNA editing activity
GO:0001217 - DNA-binding transcription repressor activity
GO:0002196 - Ser-tRNA(Ala) hydrolase activity
biological_process GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity
GO:0006400 - tRNA modification
GO:0006412 - translation
GO:0043039 - tRNA aminoacylation
GO:0045892 - negative regulation of transcription, DNA-templated
GO:0006419 - alanyl-tRNA aminoacylation
Note(s): Note(s): ...[more].
Reference(s): [1] Banerjee B., et al., 2014
[2] Guo M., et al., 2010
[3] Liu Z., et al., 2015
[4] Sun L., et al., 2016
[5] Yao P., et al., 2014
External database links:  
DIP:
 DIP-9080N
ECOCYC:
 ALAS-MONOMER
ECOLIWIKI:
 b2697
INTERPRO:
 IPR002318
INTERPRO:
 IPR018164
INTERPRO:
 IPR018165
INTERPRO:
 IPR023033
INTERPRO:
 IPR018163
INTERPRO:
 IPR003156
INTERPRO:
 IPR018162
INTERPRO:
 IPR012947
INTERPRO:
 IPR009000
MINT:
 MINT-1266342
MODBASE:
 P00957
PANTHER:
 PTHR11777:SF6
PDB:
 3HY0
PDB:
 3HY1
PDB:
 3HXZ
PDB:
 3HXW
PDB:
 3HXU
PDB:
 3HXV
PDB:
 3HXX
PDB:
 3HXY
PFAM:
 PF02272
PFAM:
 PF01411
PFAM:
 PF07973
PRIDE:
 P00957
PRINTS:
 PR00980
PRODB:
 PRO_000022074
PROSITE:
 PS50860
REFSEQ:
 NP_417177
SMART:
 SM00863
SMR:
 P00957
SWISSMODEL:
 P00957
UNIPROT:
 P00957


Operon      
Name: alaS         
Operon arrangement:
Transcription unit        Promoter
alaS


Transcriptional Regulation      
Display Regulation             
Repressed by: AlaS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3006 2819585 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_3007 (cluster) 2819687 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [6]
  promoter TSS_3008 2821435 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_3009 2822024 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_3010 2822039 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_3011 2822087 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Banerjee B., Banerjee R., 2014, Guanidine hydrochloride mediated denaturation of E. coli Alanyl-tRNA synthetase: identification of an inactive dimeric intermediate., Protein J 33(2):119-27

 [2] Guo M., Shapiro R., Schimmel P., Yang XL., 2010, Introduction of a leucine half-zipper engenders multiple high-quality crystals of a recalcitrant tRNA synthetase., Acta Crystallogr D Biol Crystallogr 66(Pt 3):243-50

 [3] Liu Z., Vargas-Rodriguez O., Goto Y., Novoa EM., Ribas de Pouplana L., Suga H., Musier-Forsyth K., 2015, Homologous trans-editing factors with broad tRNA specificity prevent mistranslation caused by serine/threonine misactivation., Proc Natl Acad Sci U S A 112(19):6027-32

 [4] Sun L., Gomes AC., He W., Zhou H., Wang X., Pan DW., Schimmel P., Pan T., Yang XL., 2016, Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a G4:U69 Base Pair., J Am Chem Soc 138(39):12948-12955

 [5] Yao P., Poruri K., Martinis SA., Fox PL., 2014, Non-catalytic regulation of gene expression by aminoacyl-tRNA synthetases., Top Curr Chem 344:167-87

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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