RegulonDB RegulonDB 10.6.3: Gene Form
   

ampC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ampC blc frdD gdx anti-anti-terminator anti-terminator terminator anti-anti-terminator anti-terminator terminator TSS_4970 (cluster) TSS_4970 (cluster) TSS_4969 TSS_4969 TSS_4968 TSS_4968 ampCp ampCp blcp blcp

Gene      
Name: ampC    Texpresso search in the literature
Synonym(s): ECK4146, EG10040, ampA, b4150
Genome position(nucleotides): 4377811 <-- 4378944 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.18
External database links:  
ASAP:
ABE-0013592
CGSC:
1033
ECHOBASE:
EB0038
ECOCYC:
EG10040
ECOLIHUB:
ampC
OU-MICROARRAY:
b4150
REGULONDB:
b4150
STRING:
511145.b4150
M3D: ampC
COLOMBOS: ampC
PortEco: b4150


Product      
Name: β-lactamase
Synonym(s): AmpA, AmpC
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 41.556
Isoelectric point: 9.077
Motif(s):
 
Type Positions Sequence
331 -> 333 KTG
1 -> 19 MFKTTLCALLITASCSTFA
20 -> 377 APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ
80 -> 80 S
166 -> 166 Y

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0016787 - hydrolase activity
GO:0008800 - beta-lactamase activity
biological_process GO:0046677 - response to antibiotic
GO:0017001 - antibiotic catabolic process
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
[IEP] Inferred from expression pattern
[IGI] Inferred from genetic interaction
[IMP] Inferred from mutant phenotype
Reference(s): [1] Eriksson-Grennberg KG., et al., 1965
[2] Grundstrom T., et al., 1980
[3] Henderson TA., et al., 1997
[4] Linstrom EB., et al., 1970
[5] Normark S., et al., 1977
[6] Santos JM., et al., 2002
External database links:  
ECOCYC:
EG10040-MONOMER
ECOLIWIKI:
b4150
INTERPRO:
IPR012338
INTERPRO:
IPR001466
INTERPRO:
IPR001586
MODBASE:
P00811
PDB:
3IXD
PDB:
3IXG
PDB:
3IXH
PDB:
3O86
PDB:
3O87
PDB:
3O88
PDB:
4E3I
PDB:
4E3J
PDB:
4E3K
PDB:
4E3L
PDB:
4E3M
PDB:
4E3N
PDB:
4E3O
PDB:
4JXG
PDB:
4JXS
PDB:
4JXV
PDB:
4JXW
PDB:
4KEN
PDB:
4KG2
PDB:
4KG5
PDB:
4KG6
PDB:
4KZ3
PDB:
4KZ4
PDB:
4KZ5
PDB:
4KZ6
PDB:
4KZ7
PDB:
4KZ8
PDB:
4KZ9
PDB:
4KZA
PDB:
4KZB
PDB:
4LV0
PDB:
4LV1
PDB:
4LV2
PDB:
4LV3
PDB:
4OKP
PDB:
4OLD
PDB:
4OLG
PDB:
5JOC
PDB:
6DPT
PDB:
6DPX
PDB:
6DPY
PDB:
6DPZ
PDB:
1C3B
PDB:
1FCM
PDB:
1FCN
PDB:
1FCO
PDB:
1FSW
PDB:
1FSY
PDB:
1GA9
PDB:
1I5Q
PDB:
1IEL
PDB:
1IEM
PDB:
1KDS
PDB:
1KDW
PDB:
1KE0
PDB:
1KE3
PDB:
1KE4
PDB:
1KVL
PDB:
1KVM
PDB:
1L0D
PDB:
1L0E
PDB:
1L0F
PDB:
1L0G
PDB:
1L2S
PDB:
1LL5
PDB:
1LL9
PDB:
1LLB
PDB:
1MXO
PDB:
1MY8
PDB:
1O07
PDB:
1PI4
PDB:
1PI5
PDB:
1XGI
PDB:
1XGJ
PDB:
2BLS
PDB:
2FFY
PDB:
2HDQ
PDB:
2HDR
PDB:
2HDS
PDB:
2HDU
PDB:
2I72
PDB:
2P9V
PDB:
2PU2
PDB:
2PU4
PDB:
2R9W
PDB:
2R9X
PDB:
2RCX
PDB:
3BLS
PDB:
3BM6
PDB:
3FKV
PDB:
3FKW
PDB:
3GQZ
PDB:
3GR2
PDB:
3GRJ
PDB:
3GSG
PDB:
3GTC
PDB:
3GV9
PDB:
3GVB
PDB:
3IWI
PDB:
3IWO
PDB:
3IWQ
PDB:
3IXB
PFAM:
PF00144
PRIDE:
P00811
PRODB:
PRO_000022095
PROSITE:
PS00336
PROTEINMODELPORTAL:
P00811
REFSEQ:
NP_418574
SMR:
P00811
UNIPROT:
P00811


Operon      
Name: ampC         
Operon arrangement:
Transcription unit        Promoter
ampC


Transcriptional Regulation      
Display Regulation             
Activated by: BolA
Repressed by: BolA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4968 4379152 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_4969 4379156 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_4970 (cluster) 4379163 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Eriksson-Grennberg KG., Boman HG., Jansson JA., Thoren S., 1965, Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants., J Bacteriol 90(1):54-62

 [2] Grundstrom T., Jaurin B., Edlund T., Normark S., 1980, Physical mapping and expression of hybrid plasmids carrying chromosomal beta-lactamase genes of Escherichia coli K-12., J Bacteriol 143(3):1127-34

 [3] Henderson TA., Young KD., Denome SA., Elf PK., 1997, AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli., J Bacteriol 179(19):6112-21

 [4] Linstrom EB., Boman HG., Steele BB., 1970, Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains., J Bacteriol 101(1):218-31

 [5] Normark S., Burman LG., 1977, Resistance of Escherichia coli to penicillins: fine-structure mapping and dominance of chromosomal beta-lactamase mutations., J Bacteriol 132(1):1-7

 [6] Santos JM., Lobo M., Matos AP., De Pedro MA., Arraiano CM., 2002, The gene bolA regulates dacA (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli., Mol Microbiol 45(6):1729-40

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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