RegulonDB RegulonDB 11.1: Gene Form
   

appA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

etk appA appX appAp appAp

Gene      
Name: appA    Texpresso search in the literature
Synonym(s): ECK0971, EG10049, b0980
Genome position(nucleotides): 1040617 --> 1041915
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.89
External database links:  
ASAP:
 ABE-0003305
CGSC:
 17758
ECHOBASE:
 EB0047
ECOLIHUB:
 appA
OU-MICROARRAY:
 b0980
STRING:
 511145.b0980
COLOMBOS: appA


Product      
Name: periplasmic phosphoanhydride phosphatase/multiple inositol-polyphosphate phosphatase
Synonym(s): AppA
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 47.057
Isoelectric point: 6.707
Motif(s):
 
Type Positions Sequence Comment
1 -> 22 MKAILIPFLSLLIPLTPQSAFA
23 -> 432 QSEPELKLESVVIVSRHGVRAPTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQFYLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL UniProt: Periplasmic AppA protein.
29 -> 374 KLESVVIVSRHGVRAPTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQFYLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSL
38 -> 38 R R → A: loss of function mutation (with PNPP, tripolyphosphate or fructose 1,6-diphosphate as substrate)
39 -> 39 H H → N: loss of function mutation (with PNPP, tripolyphosphate or fructose 1,6-diphosphate as substrate)

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0016787 - hydrolase activity
GO:0008252 - nucleotidase activity
GO:0016791 - phosphatase activity
GO:0050308 - sugar-phosphatase activity
GO:0003993 - acid phosphatase activity
GO:0008707 - 4-phytase activity
GO:0052745 - inositol phosphate phosphatase activity
biological_process GO:0016311 - dephosphorylation
GO:0016036 - cellular response to phosphate starvation
GO:0071454 - cellular response to anoxia
GO:0033518 - myo-inositol hexakisphosphate dephosphorylation
Note(s): Note(s): ...[more].
Reference(s): [1] Dassa E., et al., 1985
[2] Dassa J., et al., 1990
External database links:  
ALPHAFOLD:
 P07102
ECOCYC:
 APPA-MONOMER
ECOLIWIKI:
 b0980
INTERPRO:
 IPR000560
INTERPRO:
 IPR033379
INTERPRO:
 IPR029033
MODBASE:
 P07102
PDB:
 1DKN
PDB:
 1DKQ
PDB:
 4TSR
PDB:
 1DKO
PDB:
 1DKP
PDB:
 1DKL
PDB:
 1DKM
PFAM:
 PF00328
PRIDE:
 P07102
PRODB:
 PRO_000022109
PROSITE:
 PS00616
PROSITE:
 PS00778
REFSEQ:
 NP_415500
SMR:
 P07102
UNIPROT:
 P07102


Operon      
Name: appCBXA         
Operon arrangement:
Transcription unit        Promoter
appCBA-yccB
appCBA-yccB
appA


Transcriptional Regulation      
Display Regulation             
Activated by: ArcA, YdeO, AppY


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Dassa E., Boquet PL., 1985, Identification of the gene appA for the acid phosphatase (pH optimum 2.5) of Escherichia coli., Mol Gen Genet 200(1):68-73

 [2] Dassa J., Marck C., Boquet PL., 1990, The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase., J Bacteriol 172(9):5497-500

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