Gene
Name:	argS
Synonym(s):	ECK1877, EG10071, b1876, lov, lov-1
Genome position(nucleotides):	1960062 --> 1961795 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.29
External database links:

ASAP:	ABE-0006264
CGSC:	1010
ECHOBASE:	EB0069
MIM:	616140
OU-MICROARRAY:	b1876
PortEco:	argS
STRING:	511145.b1876
COLOMBOS:	argS

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yecMp3	1959872	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.966. P-value: 7.713e-5 Read more >	[ICWHO]	[21]
promoter	TSS_2223	1959873	reverse	nd	[RS-EPT-CBR]	[22]
promoter	yecMp2	1959984	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.41. P-value: 2.232e-5 Read more >	[ICWHO]	[21]
promoter	TSS_2224	1961626	forward	nd	[RS-EPT-CBR]	[22]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Airas RK., 1996, Differences in the magnesium dependences of the class I and class II aminoacyl-tRNA synthetases from Escherichia coli., Eur J Biochem 240(1):223-31
[2] Charlier J., 1976, On the behaviour of arginyl-tRNA synthetase of Escherichia coli in the presence of arginine precursors., Arch Int Physiol Biochim 84(3):588-90
[3] Cheng XD., Lin SX., Shi JP., Wang YL., 1991, Arginyl-tRNA synthetase from Escherichia coli affinity labeling with 3'-oxidized tRNA(Arg)., Sci China B 34(3):297-305
[4] Cooper PH., Hirshfield IN., Maas WK., 1969, Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12., Mol Gen Genet 104(4):383-90
[5] Faanes R., Rogers P., 1972, Repression of enzymes of arginine biosynthesis by L-canavanine in arginyl-transfer ribonucleic acid synthetase mutants of Escherichia coli., J Bacteriol 112(1):102-13
[6] Gerlo E., Freist W., Charlier J., 1982, Arginyl-tRNA synthetase from Escherichia coli K12: specificity with regard to ATP analogs and their magnesium complexes., Hoppe Seylers Z Physiol Chem 363(4):365-73
[7] Hirshfield IN., Horn PC., Hopwood DA., Maas WK., DeDeken R., 1968, Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants., J Mol Biol 35(1):83-93
[8] Jakubowski H., 1995, Synthesis of cysteine-containing dipeptides by aminoacyl-tRNA synthetases., Nucleic Acids Res 23(22):4608-15
[9] Liu MF., Li T., Yin ZB., Xu MG., Wang ED., Wang YL., 2000, A Strong Promoter Provided with the Gene Encoding Arginyl-tRNA Synthetase(argS) from Escherichia coli., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 32(5):435-440
[10] Liu MF., Xu MG., Xia X., Wang ED., Wang YL., 2001, A Negative Element Located in the Upstream Flanking Region of the Gene Encoding Arginyl-tRNA Synthetase (argS) from Escherichia coli., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 33(6):621-628
[11] Liu W., Liu MF., Xia X., Wang ED., Wang YL., 2002, The effect of N-terminal changes on arginyl-tRNA synthetase from Escherichia coli., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34(2):131-7
[12] Liu W., Wang ED., Wang YL., 1999, A Novel System for Hyper Expression and Rapid Purification of Arginyl-tRNA Synthetase from Escherichia coli., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 31(5):494-498
[13] Lui M., Chakraburtty K., Mehler AH., 1978, Partial reactions of aminoacyl-tRNA synthetases as functions of pH., J Biol Chem 253(22):8061-4
[14] Mehler AH., Mitra SK., 1967, The activation of arginyl transfer ribonucleic acid synthetase by transfer ribonucleic acid., J Biol Chem 242(23):5495-9
[15] Mitra SK., Mehler AH., 1967, The arginyl transfer ribonucleic acid synthetase of Escherichia coli., J Biol Chem 242(23):5490-4
[16] Neidhardt FC., Bloch PL., Pedersen S., Reeh S., 1977, Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli., J Bacteriol 129(1):378-87
[17] Reeh S., Pedersen S., Neidhardt FC., 1977, Transient rates of synthesis of five amionacyl-transfer ribonucleic acid synthetases during a shift-up of Escherichia coli., J Bacteriol 129(2):702-6
[18] Wu JF., Xia X., Wang ED., Wang YL., 1998, Study on the Overexpression of the Gene Encoding Arginyl-tRNA Synthetase under Induction., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 30(3):236-240
[19] Yem DW., Williams LS., 1971, Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors., J Bacteriol 107(2):589-91
[20] Zhang QS., Shen L., Wang ED., Wang YL., 1999, Biosynthesis and characterization of 4-fluorotryptophan-labeled Escherichia coli arginyl-tRNA synthetase., J Protein Chem 18(2):187-92
[21] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[22] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RNA cis-regulatory element

Attenuation:	Transcriptional