RegulonDB RegulonDB 10.8: Gene Form
   

aroL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aroL yaiI yaiA TrpR TrpR TyrR TyrR TyrR TSS_500 TSS_500 TSS_499 TSS_499 aroLp2 aroLp2 aroLp aroLp TSS_498 TSS_498

Gene      
Name: aroL    Texpresso search in the literature
Synonym(s): ECK0383, EG10082, b0388
Genome position(nucleotides): 406405 --> 406929 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.19
External database links:  
ASAP:
ABE-0001353
CGSC:
999
ECHOBASE:
EB0080
OU-MICROARRAY:
b0388
PortEco:
aroL
STRING:
511145.b0388
COLOMBOS: aroL


Product      
Name: shikimate kinase 2
Synonym(s): AroL, shikimate kinase II
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 19.151
Isoelectric point: 4.418
Motif(s):
 
Type Positions Sequence
2 -> 174 TQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
112 -> 126 QAAPEEDLRPTLTGK
12 -> 17 GCGKTT
11 -> 168 RGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSAL

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.20 - chorismate
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004765 - shikimate kinase activity
GO:0000287 - magnesium ion binding
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
GO:0009423 - chorismate biosynthetic process
GO:0016310 - phosphorylation
GO:0019632 - shikimate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] DeFeyter RC., et al., 1986
[2] DeFeyter RC., et al., 1986
[3] Lobner-Olesen A., et al., 1992
[4] Millar G., et al., 1986
External database links:  
ECOCYC:
AROL-MONOMER
ECOLIWIKI:
b0388
INTERPRO:
IPR027544
INTERPRO:
IPR031322
INTERPRO:
IPR027417
INTERPRO:
IPR023000
INTERPRO:
IPR000623
MODBASE:
P0A6E1
PFAM:
PF01202
PRIDE:
P0A6E1
PRINTS:
PR01100
PRODB:
PRO_000022151
PROSITE:
PS01128
REFSEQ:
NP_414922
SMR:
P0A6E1
SWISSMODEL:
P0A6E1
UNIPROT:
P0A6E1


Operon      
Name: aroL-yaiA-aroM         
Operon arrangement:
Transcription unit        Promoter
aroL-yaiA-aroM
aroL-yaiA-aroM
aroM
yaiA-aroM


Transcriptional Regulation      
Display Regulation             
Repressed by: TyrR, TrpR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_498 406276 forward nd [RS-EPT-CBR] [5]
  promoter TSS_499 406954 forward nd [RS-EPT-CBR] [5]
  promoter TSS_500 407091 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] DeFeyter RC., Davidson BE., Pittard J., 1986, Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12., J Bacteriol 165(1):233-9

 [2] DeFeyter RC., Pittard J., 1986, Purification and properties of shikimate kinase II from Escherichia coli K-12., J Bacteriol 165(1):331-3

 [3] Lobner-Olesen A., Marinus MG., 1992, Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli., J Bacteriol 174(2):525-9

 [4] Millar G., Lewendon A., Hunter MG., Coggins JR., 1986, The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product., Biochem J 237(2):427-37

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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