RegulonDB RegulonDB 11.2: Gene Form
   

cadB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cadA cadC cadB H-NS H-NS ArcA H-NS H-NS H-NS cadBp cadBp yjdLp3 yjdLp3 yjdLp4 yjdLp4

Gene      
Name: cadB    Texpresso search in the literature
Synonym(s): ECK4126, EG10132, b4132
Genome position(nucleotides): 4358697 <-- 4360031
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.44
External database links:  
ASAP:
 ABE-0013530
CGSC:
 34228
ECHOBASE:
 EB0130
ECOLIHUB:
 cadB
OU-MICROARRAY:
 b4132
STRING:
 511145.b4132
COLOMBOS: cadB


Product      
Name: lysine:cadaverine antiporter
Synonym(s): CadB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 46.665
Isoelectric point: 8.375
Motif(s):
 
Type Positions Sequence Comment
7 -> 416 IGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVS
9 -> 29 LFACTGVVAGNMMGSGIALLP
35 -> 55 IGGIAIWGWIISIIGAMSLAY UniProt: Helical.
37 -> 57 GIAIWGWIISIIGAMSLAYVY
68 -> 88 GGPIAYAGEISPAFGFQTGVL UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015297 - antiporter activity
GO:0015295 - solute:proton symporter activity
GO:0022857 - transmembrane transporter activity
GO:0043872 - lysine:cadaverine antiporter activity
biological_process GO:0006865 - amino acid transport
GO:0055085 - transmembrane transport
GO:0015839 - cadaverine transport
GO:1902600 - proton transmembrane transport
GO:1903401 - L-lysine transmembrane transport
GO:1990451 - cellular stress response to acidic pH
Note(s): Note(s): ...[more].
Reference(s): [1] Soksawatmaekhin W., et al., 2004
[2] Soksawatmaekhin W., et al., 2006
External database links:  
ALPHAFOLD:
 P0AAE8
DIP:
 DIP-48089N
ECOCYC:
 CADB-MONOMER
ECOLIWIKI:
 b4132
INTERPRO:
 IPR002293
INTERPRO:
 IPR004754
PFAM:
 PF13520
PRIDE:
 P0AAE8
REFSEQ:
 NP_418556
SMR:
 P0AAE8
UNIPROT:
 P0AAE8


Operon      
Name: cadBA         
Operon arrangement:
Transcription unit        Promoter
cadBA
cadA


Transcriptional Regulation      
Display Regulation             
Activated by: GadX, GadE-RcsB, Lrp, CadC
Repressed by: H-NS, OmpR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yjdLp4 4356507 reverse nd [COMP-AINF] [3]
  promoter yjdLp3 4356517 reverse nd [COMP-AINF] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Soksawatmaekhin W., Kuraishi A., Sakata K., Kashiwagi K., Igarashi K., 2004, Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli., Mol Microbiol 51(5):1401-12

 [2] Soksawatmaekhin W., Uemura T., Fukiwake N., Kashiwagi K., Igarashi K., 2006, Identification of the cadaverine recognition site on the cadaverine-lysine antiporter CadB., J Biol Chem 281(39):29213-20

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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