RegulonDB RegulonDB 10.8: Gene Form
   

lnt gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lnt insH3 ybeX lntp4 lntp4 insHp6 insHp6 insHp5 insHp5 insHp3 insHp3 TSS_781 TSS_781

Gene      
Name: lnt    Texpresso search in the literature
Synonym(s): ECK0649, EG10168, b0657, cutE
Genome position(nucleotides): 689343 <-- 690881 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.69
External database links:  
ASAP:
ABE-0002250
CGSC:
31471
ECHOBASE:
EB0166
OU-MICROARRAY:
b0657
PortEco:
lnt
STRING:
511145.b0657
COLOMBOS: lnt


Product      
Name: apolipoprotein N-acyltransferase
Synonym(s): CutE, Lnt
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,inner membrane
Molecular weight: 57.066
Isoelectric point: 9.736
Motif(s):
 
Type Positions Sequence
146 -> 146 F
169 -> 189 VEAINFLLMMVSGLLALALVK
237 -> 237 W
335 -> 335 K
372 -> 372 Q

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.10 - lipoprotein
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016410 - N-acyltransferase activity
GO:0016746 - transferase activity, transferring acyl groups
biological_process GO:0006807 - nitrogen compound metabolic process
GO:0042158 - lipoprotein biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Gelis-Jeanvoine S., et al., 2015
[2] Jackowski S., et al., 1986
[3] Wiktor M., et al., 2018
External database links:  
DIP:
DIP-10111N
ECOCYC:
EG10168-MONOMER
ECOLIWIKI:
b0657
INTERPRO:
IPR004563
INTERPRO:
IPR003010
INTERPRO:
IPR036526
MINT:
MINT-1238661
MODBASE:
P23930
PDB:
5XHQ
PDB:
5N6H
PDB:
5N6L
PFAM:
PF00795
PRIDE:
P23930
PRODB:
PRO_000023102
PROSITE:
PS50263
REFSEQ:
NP_415190
SMR:
P23930
UNIPROT:
P23930


Operon      
Name: ybeZYX-lnt         
Operon arrangement:
Transcription unit        Promoter
ybeZYX-lnt
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_781 689004 reverse nd [RS-EPT-CBR] [4]
  promoter insHp3 689205 reverse Similarity to the consensus
Read more >
[ICWHO] [5]
  promoter insHp5 689212 reverse Similarity to the consensus
Read more >
[ICWHO] [5]
  promoter insHp6 689222 reverse Similarity to the consensus
Read more >
[ICWHO] [5]
  promoter lntp4 690984 reverse Similarity to the consensus
Read more >
[ICWHO] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Gelis-Jeanvoine S., Lory S., Oberto J., Buddelmeijer N., 2015, Residues located on membrane-embedded flexible loops are essential for the second step of the apolipoprotein N-acyltransferase reaction., Mol Microbiol 95(4):692-705

 [2] Jackowski S., Rock CO., 1986, Transfer of fatty acids from the 1-position of phosphatidylethanolamine to the major outer membrane lipoprotein of Escherichia coli., J Biol Chem 261(24):11328-33

 [3] Wiktor M., Caffrey M., 2018, Biophysical characterization and stabilization of detergent-solubilized lipoprotein N-acyl transferase from P. aeruginosa and E. coli., Biochim Biophys Acta 1860(6):1384-1393

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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