RegulonDB RegulonDB 10.8: Gene Form
   

cysM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cysA cysM yfeS cysW anti-terminator terminator TSS_2738 TSS_2738 TSS_2737 TSS_2737 TSS_2736 TSS_2736 TSS_2735 TSS_2735 TSS_2734 TSS_2734

Gene      
Name: cysM    Texpresso search in the literature
Synonym(s): ECK2416, EG10193, b2421
Genome position(nucleotides): 2538672 <-- 2539583 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.47
External database links:  
ASAP:
 ABE-0007982
CGSC:
 17722
ECHOBASE:
 EB0190
MIM:
 236200
OU-MICROARRAY:
 b2421
PortEco:
 cysM
STRING:
 511145.b2421
COLOMBOS: cysM


Product      
Name: cysteine synthase B
Synonym(s): CysM
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.664
Isoelectric point: 5.245
Motif(s):
 
Type Positions Sequence
174 -> 178 GTTGT
5 -> 281 EQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRAALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICD

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.12 - cysteine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0004124 - cysteine synthase activity
GO:0030170 - pyridoxal phosphate binding
GO:0042803 - protein homodimerization activity
GO:0080146 - L-cysteine desulfhydrase activity
biological_process GO:0019345 - cysteine biosynthetic process via S-sulfo-L-cysteine
GO:0008652 - cellular amino acid biosynthetic process
GO:0016226 - iron-sulfur cluster assembly
GO:0019344 - cysteine biosynthetic process
GO:0006535 - cysteine biosynthetic process from serine
GO:0019450 - L-cysteine catabolic process to pyruvate
Note(s): Note(s): ...[more].
Reference(s): [1] Lynch MJ., et al., 2019
[2] Nakatani T., et al., 2012
[3] Smirnova GV., et al., 2019
[4] Uria-Nickelsen MR., et al., 1994
External database links:  
DIP:
 DIP-9383N
ECOCYC:
 ACSERLYB-MONOMER
ECOLIWIKI:
 b2421
INTERPRO:
 IPR005858
INTERPRO:
 IPR036052
INTERPRO:
 IPR005856
INTERPRO:
 IPR001926
INTERPRO:
 IPR001216
MINT:
 MINT-1300981
MODBASE:
 P16703
PANTHER:
 PTHR10314:SF100
PDB:
 2V03
PDB:
 2BHT
PDB:
 2BHS
PFAM:
 PF00291
PRIDE:
 P16703
PRODB:
 PRO_000022383
PROSITE:
 PS00901
REFSEQ:
 NP_416916
SMR:
 P16703
UNIPROT:
 P16703


Operon      
Name: cysPUWAM         
Operon arrangement:
Transcription unit        Promoter
cysPUWAM


Transcriptional Regulation      
Display Regulation             
Activated by: CysB
Repressed by: H-NS


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2734 2539529 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2735 2539540 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2736 2539646 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2737 2539652 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2738 2539704 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Lynch MJ., Crane BR., 2019, Design, Validation, and Application of an Enzyme-Coupled Hydrogen Sulfide Detection Assay., Biochemistry 58(6):474-483

 [2] Nakatani T., Ohtsu I., Nonaka G., Wiriyathanawudhiwong N., Morigasaki S., Takagi H., 2012, Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli., Microb Cell Fact 11:62

 [3] Smirnova GV., Tyulenev AV., Bezmaternykh KV., Muzyka NG., Ushakov VY., Oktyabrsky ON., 2019, Cysteine homeostasis under inhibition of protein synthesis in Escherichia coli cells., Amino Acids

 [4] Uria-Nickelsen MR., Leadbetter ER., Godchaux W., 1994, Sulfonate-sulfur utilization involves a portion of the assimilatory sulfate reduction pathway in Escherichia coli., FEMS Microbiol Lett 123(1-2):43-8

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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