RegulonDB RegulonDB 10.6.3: Gene Form
   

cysM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cysA cysM yfeS cysW anti-terminator terminator TSS_2738 TSS_2738 TSS_2737 TSS_2737 TSS_2736 TSS_2736 TSS_2735 TSS_2735 TSS_2734 TSS_2734

Gene      
Name: cysM    Texpresso search in the literature
Synonym(s): ECK2416, EG10193, b2421
Genome position(nucleotides): 2538672 <-- 2539583 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
56.47
External database links:  
ASAP:
ABE-0007982
CGSC:
17722
ECHOBASE:
EB0190
ECOCYC:
EG10193
ECOLIHUB:
cysM
MIM:
236200
OU-MICROARRAY:
b2421
REGULONDB:
b2421
STRING:
511145.b2421
M3D: cysM
COLOMBOS: cysM
PortEco: b2421


Product      
Name: cysteine synthase B
Synonym(s): CysM
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.664
Isoelectric point: 5.245
Motif(s):
 
Type Positions Sequence
174 -> 178 GTTGT
5 -> 281 EQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRAALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICD

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.12 - cysteine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0004124 - cysteine synthase activity
GO:0030170 - pyridoxal phosphate binding
GO:0080146 - L-cysteine desulfhydrase activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0019344 - cysteine biosynthetic process
GO:0006535 - cysteine biosynthetic process from serine
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-9383N
ECOCYC:
ACSERLYB-MONOMER
ECOLIWIKI:
b2421
INTERPRO:
IPR005858
INTERPRO:
IPR036052
INTERPRO:
IPR005856
INTERPRO:
IPR001926
INTERPRO:
IPR001216
MINT:
MINT-1300981
MODBASE:
P16703
PANTHER:
PTHR10314:SF100
PDB:
2BHT
PDB:
2V03
PDB:
2BHS
PFAM:
PF00291
PRIDE:
P16703
PRODB:
PRO_000022383
PROSITE:
PS00901
PROTEINMODELPORTAL:
P16703
REFSEQ:
NP_416916
SMR:
P16703
UNIPROT:
P16703


Operon      
Name: cysPUWAM         
Operon arrangement:
Transcription unit        Promoter
cysPUWAM


Transcriptional Regulation      
Display Regulation             
Activated by: CysB
Repressed by: H-NS


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2734 2539529 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2735 2539540 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2736 2539646 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2737 2539652 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2738 2539704 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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