RegulonDB RegulonDB 10.6.3: Gene Form
   

acrF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

acrF acrE yhdV terminator anti-terminator anti-anti-terminator yhdVp9 yhdVp9 TSS_3599 TSS_3599

Gene      
Name: acrF    Texpresso search in the literature
Synonym(s): ECK3253, EG10267, b3266, envD
Genome position(nucleotides): 3415033 --> 3418137 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.53
External database links:  
ASAP:
ABE-0010724
CGSC:
33608
ECHOBASE:
EB0263
ECOCYC:
EG10267
ECOLIHUB:
acrF
ECOO157CYC:
ACRF_1
ECOO157CYC:
ACRF_2
ECOO157CYC:
Z2508
OU-MICROARRAY:
b3266
REGULONDB:
b3266
STRING:
511145.b3266
M3D: acrF
COLOMBOS: acrF
PortEco: b3266


Product      
Name: multidrug efflux pump RND permease AcrF
Synonym(s): AcrF, EnvD
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 111.454
Isoelectric point: 5.118
Motif(s):
 
Type Positions Sequence
1 -> 1029 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIR
392 -> 413 TLTMFGMVLAIGLLVDDAIVVV
442 -> 460 LVGIAMVLSAVFIPMAFFG
898 -> 917 VSVMLVVPLGIVGVLLAATL
10 -> 28 IFAWVLAIILMMAGALAIL

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015562 - efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0006855 - drug transmembrane transport
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-9052N
ECOCYC:
ACRF-MONOMER
ECOLIWIKI:
b3266
ECOO157CYC:
Z2508-MONOMER
ECOO157CYC:
ACRF_2-MONOMER
ECOO157CYC:
ACRF_1-MONOMER
INTERPRO:
IPR001036
INTERPRO:
IPR004764
INTERPRO:
IPR027463
MINT:
MINT-1267966
MODBASE:
P24181
PANTHER:
PTHR32063
PFAM:
PF00873
PRIDE:
P24181
PRINTS:
PR00702
PRODB:
PRO_000022050
PROTEINMODELPORTAL:
P24181
REFSEQ:
NP_417732
SMR:
P24181
SWISSMODEL:
P24181
UNIPROT:
P24181


Operon      
Name: acrEF         
Operon arrangement:
Transcription unit        Promoter
acrEF


Transcriptional Regulation      
Display Regulation             
Activated by: FNR
Repressed by: H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3599 3417381 forward nd [RS-EPT-CBR] [1]
  promoter yhdVp9 3418196 forward Similarity to the consensus
Read more >
[ICWHO] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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