RegulonDB RegulonDB 10.8: Gene Form
   

acrF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

acrF acrE yhdV terminator anti-terminator anti-anti-terminator yhdVp9 yhdVp9 TSS_3599 TSS_3599

Gene      
Name: acrF    Texpresso search in the literature
Synonym(s): ECK3253, EG10267, b3266, envD
Genome position(nucleotides): 3415033 --> 3418137 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.53
External database links:  
ASAP:
ABE-0010724
CGSC:
33608
ECHOBASE:
EB0263
ECOO157CYC:
ACRF_2
ECOO157CYC:
Z2508
ECOO157CYC:
ACRF_1
OU-MICROARRAY:
b3266
PortEco:
acrF
STRING:
511145.b3266
COLOMBOS: acrF


Product      
Name: multidrug efflux pump RND permease AcrF
Synonym(s): AcrF, EnvD
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 111.454
Isoelectric point: 5.118
Motif(s):
 
Type Positions Sequence
1 -> 1029 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIR
392 -> 413 TLTMFGMVLAIGLLVDDAIVVV
442 -> 460 LVGIAMVLSAVFIPMAFFG
898 -> 917 VSVMLVVPLGIVGVLLAATL
10 -> 28 IFAWVLAIILMMAGALAIL

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015562 - efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
Note(s): Note(s): ...[more].
Reference(s): [1] Alon Cudkowicz N., et al., 2019
[2] Pu Y., et al., 2016
External database links:  
DIP:
DIP-9052N
ECOCYC:
ACRF-MONOMER
ECOLIWIKI:
b3266
ECOO157CYC:
ACRF_2-MONOMER
ECOO157CYC:
ACRF_1-MONOMER
ECOO157CYC:
Z2508-MONOMER
INTERPRO:
IPR001036
INTERPRO:
IPR027463
INTERPRO:
IPR004764
MINT:
MINT-1267966
MODBASE:
P24181
PANTHER:
PTHR32063
PFAM:
PF00873
PRIDE:
P24181
PRINTS:
PR00702
PRODB:
PRO_000022050
REFSEQ:
NP_417732
SMR:
P24181
SWISSMODEL:
P24181
UNIPROT:
P24181


Operon      
Name: acrEF         
Operon arrangement:
Transcription unit        Promoter
acrEF


Transcriptional Regulation      
Display Regulation             
Activated by: FNR
Repressed by: H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3599 3417381 forward nd [RS-EPT-CBR] [3]
  promoter yhdVp9 3418196 forward Similarity to the consensus
Read more >
[ICWHO] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828

 [2] Pu Y., Zhao Z., Li Y., Zou J., Ma Q., Zhao Y., Ke Y., Zhu Y., Chen H., Baker MAB., Ge H., Sun Y., Xie XS., Bai F., 2016, Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells., Mol Cell 62(2):284-294

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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