RegulonDB

Gene
Name:	fecA
Synonym(s):	ECK4281, EG10286, b4291
Genome position(nucleotides):	4514353 <-- 4516677 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	58.54
External database links:

ASAP:	ABE-0014067
CGSC:	783
ECHOBASE:	EB0282
ECOLIHUB:	fecA
ECOO157CYC:	Z1178
ECOO157CYC:	Z4386
ECOO157CYC:	Z1617
OU-MICROARRAY:	b4291
STRING:	511145.b4291
COLOMBOS:	fecA

Gene

Name:

fecA

Synonym(s):

ECK4281, EG10286, b4291

Genome position(nucleotides):

4514353 <-- 4516677 Genome Browser

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

58.54

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

ECOO157CYC:

ECOO157CYC:

ECOO157CYC:

OU-MICROARRAY:

STRING:

COLOMBOS:

Shine dalgarno
Sequence:	aaatgatgatGGGGAAggTAT

Shine dalgarno

Sequence:

aaatgatgatGGGGAAggTAT

Product

Name:

ferric citrate outer membrane transporter

Synonym(s):

FecA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

85.321

Isoelectric point:

5.753

Motif(s):

Type	Positions	Sequence
	300 -> 300	T
	757 -> 774	GIYAGQPRTLYMQGSLKF
	16 -> 16	T
	190 -> 190	A
	1 -> 33	MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFA

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.1 - Channel-type Transporters --> 4.1.B - Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)
		5 - cell processes --> 5.5 - adaptations --> 5.5.7 - Fe aquisition
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0019867 - outer membrane GO:0016020 - membrane GO:0016021 - integral component of membrane GO:0045203 - integral component of cell outer membrane
	molecular_function	GO:0005515 - protein binding GO:0015091 - ferric iron transmembrane transporter activity GO:0038023 - signaling receptor activity GO:0015344 - siderophore uptake transmembrane transporter activity
	biological_process	GO:1990641 - response to iron ion starvation GO:0023019 - signal transduction involved in regulation of gene expression GO:0006355 - regulation of transcription, DNA-templated GO:0006811 - ion transport GO:0006826 - iron ion transport GO:0055072 - iron ion homeostasis GO:0015891 - siderophore transport GO:0034755 - iron ion transmembrane transport GO:0033214 - siderophore-dependent iron import into cell GO:0044718 - siderophore transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Chakraborty R., et al., 2007
[2] Goswami M., et al., 2018
[3] Howard SP., et al., 2001
[4] Kim M., et al., 2007
[5] Lupino KM., et al., 2018
[6] Peacock RS., et al., 2006
[7] Piggot TJ., et al., 2013
[8] Pressler U., et al., 1988
[9] Sauter A., et al., 2004
[10] Thomas MD., et al., 2021
[11] Welz D., et al., 1998
[12] Zhou XH., et al., 1993
[13] Zhou XH., et al., 1995

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

ECOO157CYC:

ECOO157CYC:

ECOO157CYC:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, PdhR
Repressed by:	Fur, HypT
Undetermined regulation	AscG

Transcriptional Regulation

Display Regulation

Activated by:

CRP, PdhR

Repressed by:

Fur, HypT

Undetermined regulation

AscG

Reference(s)
[1] Chakraborty R., Storey E., van der Helm D., 2007, Molecular mechanism of ferricsiderophore passage through the outer membrane receptor proteins of Escherichia coli., Biometals 20(3-4):263-74
[2] Goswami M., Narayana Rao AVSS., 2018, Transcriptome Profiling Reveals Interplay of Multifaceted Stress Response in Escherichia coli on Exposure to Glutathione and Ciprofloxacin., mSystems 3(1)
[3] Howard SP., Herrmann C., Stratilo CW., Braun V., 2001, In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli., J Bacteriol 183(20):5885-95
[4] Kim M., Fanucci GE., Cafiso DS., 2007, Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved., Proc Natl Acad Sci U S A 104(29):11975-80
[5] Lupino KM., Romano KA., Simons MJ., Gregg JT., Panepinto L., Cruz GM., Grajek L., Caputo GA., Hickman MJ., Hecht GB., 2018, A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli., BMC Microbiol 18(1):36
[6] Peacock RS., Andrushchenko VV., Demcoe AR., Gehmlich M., Lu LS., Herrero AG., Vogel HJ., 2006, Characterization of TonB interactions with the FepA cork domain and FecA N-terminal signaling domain., Biometals 19(2):127-42
[7] Piggot TJ., Holdbrook DA., Khalid S., 2013, Conformational dynamics and membrane interactions of the E. coli outer membrane protein FecA: a molecular dynamics simulation study., Biochim Biophys Acta 1828(2):284-93
[8] Pressler U., Staudenmaier H., Zimmermann L., Braun V., 1988, Genetics of the iron dicitrate transport system of Escherichia coli., J Bacteriol 170(6):2716-24
[9] Sauter A., Braun V., 2004, Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12., J Bacteriol 186(16):5303-10
[10] Thomas MD., Ewunkem AJ., Boyd S., Williams DK., Moore A., Rhinehardt KL., Van Beveren E., Yang B., Tapia A., Han J., Harrison SH., Graves JL., 2021, Too much of a good thing: Adaption to iron (II) intoxication in Escherichia coli., Evol Med Public Health 9(1):53-67
[11] Welz D., Braun V., 1998, Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein., J Bacteriol 180(9):2387-94
[12] Zhou XH., van der Helm D., Adjimani J., 1993, Purification of outer membrane iron transport receptors from Escherichia coli by fast protein liquid chromatography: FepA and FecA., Biometals 6(1):25-35
[13] Zhou XH., van der Helm D., Venkatramani L., 1995, Binding characterization of the iron transport receptor from the outer membrane of Escherichia coli (FepA): differentiation between FepA and FecA., Biometals 8(2):129-36

Reference(s)

[1] Chakraborty R., Storey E., van der Helm D., 2007, Molecular mechanism of ferricsiderophore passage through the outer membrane receptor proteins of Escherichia coli., Biometals 20(3-4):263-74

[2] Goswami M., Narayana Rao AVSS., 2018, Transcriptome Profiling Reveals Interplay of Multifaceted Stress Response in Escherichia coli on Exposure to Glutathione and Ciprofloxacin., mSystems 3(1)

[3] Howard SP., Herrmann C., Stratilo CW., Braun V., 2001, In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli., J Bacteriol 183(20):5885-95

[4] Kim M., Fanucci GE., Cafiso DS., 2007, Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved., Proc Natl Acad Sci U S A 104(29):11975-80

[5] Lupino KM., Romano KA., Simons MJ., Gregg JT., Panepinto L., Cruz GM., Grajek L., Caputo GA., Hickman MJ., Hecht GB., 2018, A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli., BMC Microbiol 18(1):36

[6] Peacock RS., Andrushchenko VV., Demcoe AR., Gehmlich M., Lu LS., Herrero AG., Vogel HJ., 2006, Characterization of TonB interactions with the FepA cork domain and FecA N-terminal signaling domain., Biometals 19(2):127-42

[7] Piggot TJ., Holdbrook DA., Khalid S., 2013, Conformational dynamics and membrane interactions of the E. coli outer membrane protein FecA: a molecular dynamics simulation study., Biochim Biophys Acta 1828(2):284-93

[8] Pressler U., Staudenmaier H., Zimmermann L., Braun V., 1988, Genetics of the iron dicitrate transport system of Escherichia coli., J Bacteriol 170(6):2716-24

[9] Sauter A., Braun V., 2004, Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12., J Bacteriol 186(16):5303-10

[10] Thomas MD., Ewunkem AJ., Boyd S., Williams DK., Moore A., Rhinehardt KL., Van Beveren E., Yang B., Tapia A., Han J., Harrison SH., Graves JL., 2021, Too much of a good thing: Adaption to iron (II) intoxication in Escherichia coli., Evol Med Public Health 9(1):53-67

[11] Welz D., Braun V., 1998, Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein., J Bacteriol 180(9):2387-94

[12] Zhou XH., van der Helm D., Adjimani J., 1993, Purification of outer membrane iron transport receptors from Escherichia coli by fast protein liquid chromatography: FepA and FecA., Biometals 6(1):25-35

[13] Zhou XH., van der Helm D., Venkatramani L., 1995, Binding characterization of the iron transport receptor from the outer membrane of Escherichia coli (FepA): differentiation between FepA and FecA., Biometals 8(2):129-36