RegulonDB RegulonDB 11.0: Gene Form
   

fhuB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fhuB hemL fhuD fhuC TSS_291 TSS_291 TSS_290 TSS_290

Gene      
Name: fhuB    Texpresso search in the literature
Synonym(s): ECK0152, EG10303, b0153
Genome position(nucleotides): 171462 --> 173444
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 59.76
External database links:  
ASAP:
 ABE-0000526
CGSC:
 776
ECHOBASE:
 EB0299
ECOLIHUB:
 fhuB
OU-MICROARRAY:
 b0153
STRING:
 511145.b0153
COLOMBOS: fhuB


Product      
Name: iron(III) hydroxamate ABC transporter membrane subunit
Synonym(s): FhuB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 70.423
Isoelectric point: 11.359
Motif(s):
 
Type Positions Sequence Comment
5 -> 25 IALFPALLLALLVIVATALTW UniProt: Helical.
11 -> 11 L UniProt: In Ref. 6; CAA29256..
17 -> 323 VIVATALTWMNFSQALPRSQWAQAAWSPDIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLGITVTTLWAIPGAMASQFAAQAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAINQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLDDGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPRLMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLL
62 -> 82 LAISLLVGAGLGLVGVLFQQV UniProt: Helical.
93 -> 113 LGVATGAQLGITVTTLWAIPG UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.7 - Fe aquisition
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0005515 - protein binding
GO:0022857 - transmembrane transporter activity
GO:0015344 - siderophore uptake transmembrane transporter activity
biological_process GO:0098711 - iron ion import across plasma membrane
GO:0006811 - ion transport
GO:0055072 - iron ion homeostasis
GO:0033214 - siderophore-dependent iron import into cell
GO:0015687 - ferric-hydroxamate import into cell
Note(s): Note(s): ...[more].
Reference(s): [1] Bohm B., et al., 1996
External database links:  
ALPHAFOLD:
 P06972
DIP:
 DIP-9603N
ECOCYC:
 FHUB-MONOMER
ECOLIWIKI:
 b0153
INTERPRO:
 IPR000522
INTERPRO:
 IPR037294
MODBASE:
 P06972
PANTHER:
 PTHR30472
PFAM:
 PF01032
PRIDE:
 P06972
PRODB:
 PRO_000022610
REFSEQ:
 NP_414695
SMR:
 P06972
UNIPROT:
 P06972


Operon      
Name: fhuACDB         
Operon arrangement:
Transcription unit        Promoter
fhuACDB


Transcriptional Regulation      
Display Regulation             
Repressed by: Fur


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_290 173420 forward nd [RS-EPT-CBR] [2]
  promoter TSS_291 173769 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Bohm B., Boschert H., Koster W., 1996, Conserved amino acids in the N- and C-terminal domains of integral membrane transporter FhuB define sites important for intra- and intermolecular interactions., Mol Microbiol 20(1):223-32

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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