RegulonDB RegulonDB 10.8: Gene Form
   

folA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

kefC apaH folA kefF IHF TyrR TyrR folAp folAp

Gene      
Name: folA    Texpresso search in the literature
Synonym(s): ECK0049, EG10326, b0048, tmr, tmrA
Genome position(nucleotides): 49823 --> 50302 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.71
External database links:  
ASAP:
 ABE-0000164
CGSC:
 744
ECHOBASE:
 EB0322
MIM:
 613839
NCBI-GENE:
 944790
OU-MICROARRAY:
 b0048
PortEco:
 folA
STRING:
 511145.b0048
COLOMBOS: folA


Product      
Name: dihydrofolate reductase
Synonym(s): FolA, Tmr, TmrA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 17.999
Isoelectric point: 4.609
Motif(s):
 
Type Positions Sequence
1 -> 158 MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILER
20 -> 20 M
42 -> 42 M
95 -> 102 GGGRVYEQ
154 -> 154 E

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.2 - folic acid
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0051870 - methotrexate binding
GO:0051871 - dihydrofolic acid binding
GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0004146 - dihydrofolate reductase activity
GO:0005542 - folic acid binding
GO:0050661 - NADP binding
GO:0070401 - NADP+ binding
GO:0070402 - NADPH binding
biological_process GO:0006545 - glycine biosynthetic process
GO:0006730 - one-carbon metabolic process
GO:0009257 - 10-formyltetrahydrofolate biosynthetic process
GO:0042493 - response to drug
GO:0046655 - folic acid metabolic process
GO:0046656 - folic acid biosynthetic process
GO:0046677 - response to antibiotic
GO:0046654 - tetrahydrofolate biosynthetic process
GO:0031427 - response to methotrexate
GO:0055114 - oxidation-reduction process
GO:0046452 - dihydrofolate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Plesa C., et al., 2018
External database links:  
DIP:
 DIP-35824N
DISPROT:
 DP00301
ECOCYC:
 DIHYDROFOLATEREDUCT-MONOMER
ECOLIWIKI:
 b0048
INTERPRO:
 IPR001796
INTERPRO:
 IPR024072
INTERPRO:
 IPR017925
INTERPRO:
 IPR012259
MINT:
 MINT-1239602
MODBASE:
 P0ABQ4
PANTHER:
 PTHR22778:SF16
PDB:
 4X5F
PDB:
 4X5G
PDB:
 4X5H
PDB:
 4X5I
PDB:
 4X5J
PDB:
 5CC9
PDB:
 5CCC
PDB:
 5DFR
PDB:
 5E8Q
PDB:
 5EAJ
PDB:
 5UIH
PDB:
 5UII
PDB:
 5UIO
PDB:
 5UIP
PDB:
 5UJX
PDB:
 5W3Q
PDB:
 5Z6F
PDB:
 5Z6J
PDB:
 5Z6K
PDB:
 5Z6L
PDB:
 5Z6M
PDB:
 6CQA
PDB:
 6DFR
PDB:
 6MR9
PDB:
 6MT8
PDB:
 6MTH
PDB:
 7DFR
PDB:
 1DDR
PDB:
 1DDS
PDB:
 1DHI
PDB:
 1DHJ
PDB:
 1DRA
PDB:
 1DRB
PDB:
 1DRE
PDB:
 1DRH
PDB:
 1DYH
PDB:
 1DYI
PDB:
 1DYJ
PDB:
 1JOL
PDB:
 1JOM
PDB:
 1RA1
PDB:
 1RA2
PDB:
 1RA3
PDB:
 1RA8
PDB:
 1RA9
PDB:
 1RB2
PDB:
 1RB3
PDB:
 1RC4
PDB:
 1RD7
PDB:
 1RE7
PDB:
 1RF7
PDB:
 1RG7
PDB:
 1RH3
PDB:
 1RX1
PDB:
 1RX2
PDB:
 1RX3
PDB:
 1RX4
PDB:
 1RX5
PDB:
 1RX6
PDB:
 1RX7
PDB:
 1RX8
PDB:
 1RX9
PDB:
 1TDR
PDB:
 2ANO
PDB:
 2ANQ
PDB:
 2D0K
PDB:
 2DRC
PDB:
 2INQ
PDB:
 3DAU
PDB:
 3DRC
PDB:
 3K74
PDB:
 3KFY
PDB:
 3OCH
PDB:
 3QL3
PDB:
 3QYL
PDB:
 3QYO
PDB:
 3R33
PDB:
 4DFR
PDB:
 4EIG
PDB:
 4EIZ
PDB:
 4EJ1
PDB:
 4FHB
PDB:
 4GH8
PDB:
 4I13
PDB:
 4I1N
PDB:
 4KJJ
PDB:
 4KJK
PDB:
 4KJL
PDB:
 4NX6
PDB:
 4NX7
PDB:
 4PDJ
PFAM:
 PF00186
PRIDE:
 P0ABQ4
PRINTS:
 PR00070
PROSITE:
 PS00075
PROSITE:
 PS51330
REFSEQ:
 NP_414590
SMR:
 P0ABQ4
UNIPROT:
 P0ABQ4


Operon      
Name: folA         
Operon arrangement:
Transcription unit        Promoter
folA


Transcriptional Regulation      
Display Regulation             
Activated by: TyrR, IHF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Plesa C., Sidore AM., Lubock NB., Zhang D., Kosuri S., 2018, Multiplexed gene synthesis in emulsions for exploring protein functional landscapes., Science 359(6373):343-347

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