RegulonDB RegulonDB 10.8: Gene Form
   

folC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

folC accD dedD cvpA PurR anti-terminator terminator TSS_2623 TSS_2623 TSS_2622 TSS_2622 TSS_2621 TSS_2621 dedDp3 dedDp3 dedDp2 dedDp2 cvpAp1 cvpAp1 cvpAp2 cvpAp2

Gene      
Name: folC    Texpresso search in the literature
Synonym(s): ECK2309, EG10327, b2315, dedC
Genome position(nucleotides): 2431674 <-- 2432942 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.03
Reference(s): [1] Bognar AL., et al., 1987
External database links:  
ASAP:
 ABE-0007645
CGSC:
 17704
ECHOBASE:
 EB0323
ENTREZ:
 M32445
NCBI-GENE:
 945451
OU-MICROARRAY:
 b2315
PortEco:
 folC
STRING:
 511145.b2315
COLOMBOS: folC


Product      
Name: bifunctional folylpolyglutamate synthetase / dihydrofolate synthetase
Synonym(s): DedC, FolC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 45.406
Isoelectric point: 5.588
Motif(s):
 
Type Positions Sequence
54 -> 197 VAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPESAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKP
328 -> 328 A
154 -> 154 D
153 -> 155 LDA
155 -> 155 A

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.2 - folic acid
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004326 - tetrahydrofolylpolyglutamate synthase activity
GO:0008841 - dihydrofolate synthase activity
biological_process GO:0006730 - one-carbon metabolic process
GO:0009058 - biosynthetic process
GO:0009257 - 10-formyltetrahydrofolate biosynthetic process
GO:0009396 - folic acid-containing compound biosynthetic process
GO:0046656 - folic acid biosynthetic process
GO:0046654 - tetrahydrofolate biosynthetic process
GO:0006761 - dihydrofolate biosynthetic process
GO:0046901 - tetrahydrofolylpolyglutamate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-9674N
ECOCYC:
 FOLC-MONOMER
ECOLIWIKI:
 b2315
INTERPRO:
 IPR004101
INTERPRO:
 IPR036615
INTERPRO:
 IPR036565
INTERPRO:
 IPR018109
INTERPRO:
 IPR013221
INTERPRO:
 IPR001645
MINT:
 MINT-1301447
MODBASE:
 P08192
PANTHER:
 PTHR11136
PDB:
 1W7K
PDB:
 1W78
PFAM:
 PF02875
PFAM:
 PF08245
PRIDE:
 P08192
PRODB:
 PRO_000022678
PROSITE:
 PS01012
PROSITE:
 PS01011
REFSEQ:
 NP_416818
SMR:
 P08192
UNIPROT:
 P08192


Operon      
Name: folC-dedD         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter dedDp2 2431868 reverse Similarity to the consensus
Read more > 		[ICWHO] [2]
  promoter dedDp3 2431875 reverse Similarity to the consensus
Read more > 		[ICWHO] [2]
  promoter TSS_2621 2433655 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2622 2433908 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2623 2433926 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Bognar AL., Osborne C., Shane B., 1987, Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene., J Biol Chem 262(25):12337-43

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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