RegulonDB RegulonDB 11.1: Gene Form
   

frdA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

frdA epmA frdB frdC DcuR FNR NarL NarL NarL NarL NarL anti-terminator anti-anti-terminator TSS_4998 TSS_4998 epmAp epmAp TSS_4996 (cluster) TSS_4996 (cluster) frdAp2 frdAp2 frdAp frdAp TSS_4995 (cluster) TSS_4995 (cluster) TSS_4994 TSS_4994 TSS_4993 (cluster) TSS_4993 (cluster) TSS_4992 TSS_4992 TSS_4991 (cluster) TSS_4991 (cluster) TSS_4990 (cluster) TSS_4990 (cluster) TSS_4989 TSS_4989 TSS_4988 TSS_4988 TSS_4987 TSS_4987 TSS_4986 TSS_4986 TSS_4985 TSS_4985 TSS_4984 TSS_4984 TSS_4983 TSS_4983 TSS_4982 TSS_4982 TSS_4981 TSS_4981 TSS_4980 (cluster) TSS_4980 (cluster) TSS_4979 TSS_4979 TSS_4978 TSS_4978 TSS_4977 TSS_4977 TSS_4976 TSS_4976 TSS_4975 (cluster) TSS_4975 (cluster) TSS_4974 TSS_4974 TSS_4973 TSS_4973 TSS_4972 TSS_4972 frdDp7 frdDp7 frdDp1 frdDp1 TSS_4971 TSS_4971 frdDp2 frdDp2

Gene      
Name: frdA    Texpresso search in the literature
Synonym(s): ECK4150, EG10330, b4154
Genome position(nucleotides): 4380510 <-- 4382318
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.38
External database links:  
ASAP:
 ABE-0013604
CGSC:
 742
ECHOBASE:
 EB0326
ECOLIHUB:
 frdA
OU-MICROARRAY:
 b4154
STRING:
 511145.b4154
COLOMBOS: frdA


Product      
Name: fumarate reductase flavoprotein subunit
Synonym(s): FrdA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 65.972
Isoelectric point: 6.237
Motif(s):
 
Type Positions Sequence Comment
2 -> 602 QTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSHTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCPWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG UniProt: Fumarate reductase flavoprotein subunit.
7 -> 397 DLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSHTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCPWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSL
12 -> 16 GAGGA UniProt: FAD.
36 -> 38 ISK UniProt: FAD.
44 -> 52 SHTVAAEGG UniProt: FAD.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.2 - electron acceptors
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0045284 - plasma membrane fumarate reductase complex
molecular_function GO:0102040 - fumarate reductase (menaquinone)
GO:0005515 - protein binding
GO:0009055 - electron transfer activity
GO:0016491 - oxidoreductase activity
GO:0000166 - nucleotide binding
GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors
GO:0050660 - flavin adenine dinucleotide binding
GO:0000104 - succinate dehydrogenase activity
GO:0071949 - FAD binding
biological_process GO:0006113 - fermentation
GO:0006974 - cellular response to DNA damage stimulus
GO:0009061 - anaerobic respiration
GO:0001539 - cilium or flagellum-dependent cell motility
GO:0022900 - electron transport chain
GO:0044780 - bacterial-type flagellum assembly
Note(s): Note(s): ...[more].
Reference(s): [1] Maklashina E., et al., 2018
External database links:  
ALPHAFOLD:
 P00363
DIP:
 DIP-9681N
ECOCYC:
 FUM-FLAVO
ECOLIWIKI:
 b4154
INTERPRO:
 IPR027477
INTERPRO:
 IPR005884
INTERPRO:
 IPR003953
INTERPRO:
 IPR003952
INTERPRO:
 IPR011280
INTERPRO:
 IPR036188
INTERPRO:
 IPR014006
INTERPRO:
 IPR015939
INTERPRO:
 IPR037099
MODBASE:
 P00363
PANTHER:
 PTHR11632:SF53
PDB:
 6B58
PDB:
 6AWF
PDB:
 5VPN
PDB:
 4KX6
PDB:
 3P4S
PDB:
 3P4R
PDB:
 3P4Q
PDB:
 3P4P
PDB:
 3CIR
PDB:
 2B76
PDB:
 1L0V
PDB:
 1KF6
PDB:
 1KFY
PFAM:
 PF02910
PFAM:
 PF00890
PRIDE:
 P00363
PRODB:
 PRO_000022686
PROSITE:
 PS00504
REFSEQ:
 NP_418578
SMR:
 P00363
UNIPROT:
 P00363


Operon      
Name: frdABCD         
Operon arrangement:
Transcription unit        Promoter
frdABCD
frdABCD


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, DcuR
Repressed by: NarL


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter frdDp2 4379433 reverse nd [COMP-AINF] [2]
  promoter TSS_4971 4379467 reverse nd [RS-EPT-CBR] [3]
  promoter frdDp1 4379473 reverse nd [COMP-AINF] [2]
  promoter frdDp7 4379474 reverse nd [COMP-AINF] [2]
  promoter TSS_4972 4379642 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4973 4380402 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4974 4380562 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4975 (cluster) 4380608 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4976 4380764 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4977 4380768 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4978 4380783 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4979 4380790 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4980 (cluster) 4380795 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4981 4380800 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4982 4380895 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4983 4381317 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4984 4381338 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4985 4381346 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4986 4381467 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4987 4381476 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4988 4381624 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4989 4382039 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4990 (cluster) 4382309 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4991 (cluster) 4382324 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4992 4382327 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4993 (cluster) 4382334 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4994 4382336 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4995 (cluster) 4382398 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4996 (cluster) 4382418 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4998 4383369 forward nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Maklashina E., Rajagukguk S., Iverson TM., Cecchini G., 2018, The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS., J Biol Chem 293(20):7754-7765

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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