RegulonDB

Gene
Name:	ftsI
Synonym(s):	ECK0085, EG10341, b0084, pbpB, sep
Genome position(nucleotides):	91413 --> 93179 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.99
External database links:

ASAP:	ABE-0000309
CGSC:	423
ECHOBASE:	EB0337
ECOLIHUB:	ftsI
OU-MICROARRAY:	b0084
STRING:	511145.b0084
COLOMBOS:	ftsI

Gene

Name:

ftsI

Synonym(s):

ECK0085, EG10341, b0084, pbpB, sep

Genome position(nucleotides):

91413 --> 93179 Genome Browser

Strand:

forward

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

53.99

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

23 -> 23

61 -> 61

70 -> 220

SRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIED

307 -> 307

57 -> 57

Type

Positions

Sequence

23 -> 23

61 -> 61

70 -> 220

SRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIED

307 -> 307

57 -> 57

578 -> 588

INQGEGTGGRS

1 -> 577

MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFV

62 -> 62

19 -> 39

FISWRFALLCGCILLALAFLL

260 -> 554

GSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIM

361 -> 361

46 -> 46

39 -> 39

210 -> 210

Multifun Terms (GenProtEC)

1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)

5 - cell processes --> 5.1 - cell division

5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity

6 - cell structure --> 6.2 - murein

Gene Ontology Terms (GO)

cellular_component

GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0031226 - intrinsic component of plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0032153 - cell division site

molecular_function

GO:0005515 - protein binding
GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0004180 - carboxypeptidase activity
GO:0009002 - serine-type D-Ala-D-Ala carboxypeptidase activity
GO:0008658 - penicillin binding

biological_process

GO:0051301 - cell division
GO:0006508 - proteolysis
GO:0007049 - cell cycle
GO:0009252 - peptidoglycan biosynthetic process
GO:0009410 - response to xenobiotic stimulus
GO:0043093 - FtsZ-dependent cytokinesis
GO:0000917 - division septum assembly
GO:0008360 - regulation of cell shape
GO:0071555 - cell wall organization

Transcriptional Regulation
	Display Regulation
Repressed by:	MraZ, LexA, PdhR

Transcriptional Regulation

Display Regulation

Repressed by:

MraZ, LexA, PdhR

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_159	92485	forward	nd	[RS-EPT-CBR]	[9]
promoter	TSS_160	94608	forward	nd	[RS-EPT-CBR]	[9]
promoter	TSS_161 (cluster)	94681	forward	nd	[RS-EPT-CBR]	[9]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_159

92485

forward

[RS-EPT-CBR]

[9]

promoter

TSS_160

94608

forward

[RS-EPT-CBR]

[9]

promoter

TSS_161 (cluster)

94681

forward

[RS-EPT-CBR]

[9]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Barbati S., Grenga L., Luzi G., Paolozzi L., Ghelardini P., 2010, Prokaryotic division interactome: setup of an assay for protein-protein interaction mutant selection., Res Microbiol 161(2):118-26
[2] Boes A., Olatunji S., Breukink E., Terrak M., 2019, Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN., MBio 10(1)
[3] Botta GA., Park JT., 1981, Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation., J Bacteriol 145(1):333-40
[4] Corbin BD., Geissler B., Sadasivam M., Margolin W., 2004, Z-ring-independent interaction between a subdomain of FtsA and late septation proteins as revealed by a polar recruitment assay., J Bacteriol 186(22):7736-44
[5] Di Lallo G., Fagioli M., Barionovi D., Ghelardini P., Paolozzi L., 2003, Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation., Microbiology 149(Pt 12):3353-9
[6] Ishino F., Matsuhashi M., 1981, Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence., Biochem Biophys Res Commun 101(3):905-11
[7] Jahn LJ., Munck C., Ellabaan MMH., Sommer MOA., 2017, Adaptive Laboratory Evolution of Antibiotic Resistance Using Different Selection Regimes Lead to Similar Phenotypes and Genotypes., Front Microbiol 8:816
[8] Soderstrom B., Chan H., Daley DO., 2019, Super-resolution images of peptidoglycan remodelling enzymes at the division site of Escherichia coli., Curr Genet 65(1):99-101
[9] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Barbati S., Grenga L., Luzi G., Paolozzi L., Ghelardini P., 2010, Prokaryotic division interactome: setup of an assay for protein-protein interaction mutant selection., Res Microbiol 161(2):118-26

[2] Boes A., Olatunji S., Breukink E., Terrak M., 2019, Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN., MBio 10(1)

[3] Botta GA., Park JT., 1981, Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation., J Bacteriol 145(1):333-40

[4] Corbin BD., Geissler B., Sadasivam M., Margolin W., 2004, Z-ring-independent interaction between a subdomain of FtsA and late septation proteins as revealed by a polar recruitment assay., J Bacteriol 186(22):7736-44

[5] Di Lallo G., Fagioli M., Barionovi D., Ghelardini P., Paolozzi L., 2003, Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation., Microbiology 149(Pt 12):3353-9

[6] Ishino F., Matsuhashi M., 1981, Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence., Biochem Biophys Res Commun 101(3):905-11

[7] Jahn LJ., Munck C., Ellabaan MMH., Sommer MOA., 2017, Adaptive Laboratory Evolution of Antibiotic Resistance Using Different Selection Regimes Lead to Similar Phenotypes and Genotypes., Front Microbiol 8:816

[8] Soderstrom B., Chan H., Daley DO., 2019, Super-resolution images of peptidoglycan remodelling enzymes at the division site of Escherichia coli., Curr Genet 65(1):99-101

[9] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.