RegulonDB

Gene
Name:	fumA
Synonym(s):	ECK1607, EG10356, b1612
Genome position(nucleotides):	1686731 <-- 1688377 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.55
External database links:

ASAP:	ABE-0005392
CGSC:	18292
ECHOBASE:	EB0351
OU-MICROARRAY:	b1612
PortEco:	fumA
STRING:	511145.b1612
COLOMBOS:	fumA

Product

Name:

fumarase A

Synonym(s):

FumA

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

60.299

Isoelectric point:

6.517

Motif(s):

Type	Positions	Sequence
	51 -> 326	ARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKA
	2 -> 548	SNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK
	332 -> 537	GIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQ

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
Gene Ontology Terms (GO)
	molecular_function	GO:0003824 - catalytic activity GO:0016829 - lyase activity GO:0016853 - isomerase activity GO:0046872 - metal ion binding GO:0016836 - hydro-lyase activity GO:0051536 - iron-sulfur cluster binding GO:0051539 - 4 iron, 4 sulfur cluster binding GO:0004333 - fumarate hydratase activity GO:0042802 - identical protein binding GO:0042803 - protein homodimerization activity GO:0050163 - oxaloacetate tautomerase activity
	biological_process	GO:0006091 - generation of precursor metabolites and energy GO:0006099 - tricarboxylic acid cycle

Note(s):

Note(s): ...[more].

Reference(s):

[1] Calderon IL., et al., 2009
[2] Gray CT., et al., 1966

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

fumAC

Operon arrangement:

Transcription unit	Promoter
fumC	fumCp1
fumC	fumCp
fumA	fumAp
fumAC	fumAp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	FNR, ArcA

Regulation by small RNA
	Display Regulation
small RNA	ryhB

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1895	1688441		reverse	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Calderon IL., Elias AO., Fuentes EL., Pradenas GA., Castro ME., Arenas FA., Perez JM., Vasquez CC., 2009, Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases., Microbiology 155(Pt 6):1840-6
[2] Gray CT., Wimpenny JW., Mossman MR., 1966, Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli., Biochim Biophys Acta 117(1):33-41
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.