RegulonDB RegulonDB 10.7: Gene Form
   

gcd gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gcd cueO hpt IHF CRP TSS_268 TSS_268 TSS_267 (cluster) TSS_267 (cluster) hptp hptp gcdp2 gcdp2 gcdp1 gcdp1 TSS_265 TSS_265

Gene      
Name: gcd    Texpresso search in the literature
Synonym(s): ECK0123, EG10369, b0124
Genome position(nucleotides): 138835 <-- 141225 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.42
External database links:  
ASAP:
ABE-0000433
CGSC:
30502
ECHOBASE:
EB0364
OU-MICROARRAY:
b0124
PORTECO:
gcd
REGULONDB:
b0124
STRING:
511145.b0124
M3D: gcd
COLOMBOS: gcd
PortEco: b0124


Shine dalgarno      
Sequence: atgaattgaaATGGTGtctcttTAT


Product      
Name: quinoprotein glucose dehydrogenase
Synonym(s): Gcd
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,inner membrane
Molecular weight: 86.747
Isoelectric point: 5.296
Motif(s):
 
Type Positions Sequence
11 -> 37 LLVTLTALFAALCGLYLLIGGGWLVAI
63 -> 81 AALWLYAALLLGTMIWGVW
149 -> 156 TLSADATP
362 -> 387 SGVIRGFDVNTGELLWAFDPGAKDPN
666 -> 666 Q

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.9 - misc. glucose metabolism
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0008876 - quinoprotein glucose dehydrogenase activity
GO:0016614 - oxidoreductase activity, acting on CH-OH group of donors
GO:0000287 - magnesium ion binding
GO:0048038 - quinone binding
GO:0048039 - ubiquinone binding
GO:0070968 - pyrroloquinoline quinone binding
biological_process GO:0055114 - oxidation-reduction process
GO:0019595 - non-phosphorylated glucose catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Cozier GE., et al., 1995
[2] Yamada M., et al., 1993
External database links:  
DIP:
DIP-9747N
ECOCYC:
GLUCDEHYDROG-MONOMER
ECOLIWIKI:
b0124
INTERPRO:
IPR002372
INTERPRO:
IPR018391
INTERPRO:
IPR017511
INTERPRO:
IPR001479
INTERPRO:
IPR011047
MINT:
MINT-1267882
PANTHER:
PTHR32303:SF4
PFAM:
PF01011
PRIDE:
P15877
PRODB:
PRO_000022760
PROSITE:
PS00363
PROSITE:
PS00364
PROTEINMODELPORTAL:
P15877
REFSEQ:
NP_414666
SMART:
SM00564
SMR:
P15877
UNIPROT:
P15877


Operon      
Name: gcd         
Operon arrangement:
Transcription unit        Promoter
gcd
gcd


Transcriptional Regulation      
Display Regulation             
Activated by: IHF
Repressed by: CRP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_265 137630 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_267 (cluster) 141407 forward For this promoter, there
Read more >
[RS-EPT-CBR] [3]
  promoter TSS_268 141650 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Cozier GE., Anthony C., 1995, Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobacterium extorquens., Biochem J 312 ( Pt 3):679-85

 [2] Yamada M., Asaoka S., Saier MH., Yamada Y., 1993, Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression., J Bacteriol 175(2):568-71

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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