RegulonDB RegulonDB 10.7: Gene Form
   

glnS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nagE glnS chiP NagC CRP NagC terminator anti-terminator chiPp chiPp TSS_812 TSS_812 TSS_811 TSS_811 TSS_810 TSS_810 TSS_809 TSS_809 glnSp1 glnSp1 glnSp2 glnSp2

Gene      
Name: glnS    Texpresso search in the literature
Synonym(s): ECK0668, EG10390, b0680
Genome position(nucleotides): 706093 --> 707757 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.49
Note(s): The glnS gene expression could be repressed in a direct or indirect way by the OmpR protein. Since, using gene arrray system, a repression of this gene was observed as a result of an up mutation in the ompR gene Brombacher E,2003.
External database links:  
ASAP:
ABE-0002317
CGSC:
698
ECHOBASE:
EB0385
MIM:
615760
OU-MICROARRAY:
b0680
PORTECO:
glnS
REGULONDB:
b0680
STRING:
511145.b0680
M3D: glnS
COLOMBOS: glnS
PortEco: b0680


Product      
Name: glutamine—tRNA ligase
Synonym(s): GlnRS, GlnS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 63.478
Isoelectric point: 6.211
Motif(s):
 
Type Positions Sequence
268 -> 272 VMSKR
35 -> 37 EPN
269 -> 271 MSK
549 -> 554 WAKVGE
2 -> 554 SEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADGRKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0000166 - nucleotide binding
GO:0004819 - glutamine-tRNA ligase activity
GO:0005524 - ATP binding
biological_process GO:0006412 - translation
GO:0006418 - tRNA aminoacylation for protein translation
GO:0043039 - tRNA aminoacylation
GO:0006425 - glutaminyl-tRNA aminoacylation
GO:0006424 - glutamyl-tRNA aminoacylation
Note(s): Note(s): ...[more].
Reference(s): [1] Druart K., et al., 2017
External database links:  
DIP:
DIP-9787N
ECOCYC:
GLNS-MONOMER
ECOLIWIKI:
b0680
INTERPRO:
IPR020056
INTERPRO:
IPR014729
INTERPRO:
IPR011035
INTERPRO:
IPR004514
INTERPRO:
IPR001412
INTERPRO:
IPR000924
INTERPRO:
IPR020058
INTERPRO:
IPR020059
INTERPRO:
IPR022861
INTERPRO:
IPR020061
MINT:
MINT-1227909
MODBASE:
P00962
PANTHER:
PTHR10119
PDB:
4JXZ
PDB:
4JYZ
PDB:
4JXX
PDB:
2RE8
PDB:
2RD2
PDB:
1ZJW
PDB:
1QTQ
PDB:
1QRU
PDB:
1QRT
PDB:
1QRS
PDB:
1O0C
PDB:
1O0B
PDB:
1NYL
PDB:
1GTS
PDB:
1GTR
PDB:
1GSG
PDB:
1EXD
PDB:
1EUY
PDB:
1EUQ
PFAM:
PF00749
PFAM:
PF03950
PRIDE:
P00962
PRINTS:
PR00987
PROSITE:
PS00178
PROTEINMODELPORTAL:
P00962
REFSEQ:
NP_415206
SMR:
P00962
UNIPROT:
P00962


Operon      
Name: glnS         
Operon arrangement:
Transcription unit        Promoter
glnS
glnS


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_809 706078 forward nd [RS-EPT-CBR] [2]
  promoter TSS_810 706086 forward nd [RS-EPT-CBR] [2]
  promoter TSS_811 706185 forward nd [RS-EPT-CBR] [2]
  promoter TSS_812 706250 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Druart K., Guennec ML., Palmai Z., Simonson T., 2017, Probing the stereospecificity of tyrosyl- and glutaminyl-tRNA synthetase with molecular dynamics., J Mol Graph Model 71:192-199

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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