RegulonDB RegulonDB 10.8: Gene Form
   

glpA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glpA glpT glpB glpC GlpR Fis FNR GlpR CRP Fis GlpR FNR CRP GlpR Fis Fis GlpR CRP Fis IHF Fis IHF GlpR GlpR GlpR anti-terminator anti-anti-terminator terminator yfaDp5 yfaDp5 glpABCp glpABCp glpTQp glpTQp glpQp glpQp

Gene      
Name: glpA    Texpresso search in the literature
Synonym(s): ECK2233, EG10391, b2241
Genome position(nucleotides): 2352647 --> 2354275 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 57.34
External database links:  
ASAP:
 ABE-0007422
CGSC:
 694
ECHOBASE:
 EB0386
OU-MICROARRAY:
 b2241
PortEco:
 glpA
STRING:
 511145.b2241
COLOMBOS: glpA


Product      
Name: anaerobic glycerol-3-phosphate dehydrogenase subunit A
Synonym(s): GlpA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 58.958
Isoelectric point: 6.62
Motif(s):
 
Type Positions Sequence
10 -> 38 DVIIIGGGATGAGIARDCALRGLRVILVE
432 -> 484 LVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQ
329 -> 329 V

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.6 - misc. glycerol metabolism
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0009331 - glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0050660 - flavin adenine dinucleotide binding
GO:0004368 - glycerol-3-phosphate dehydrogenase (quinone) activity
GO:0010181 - FMN binding
GO:0052590 - sn-glycerol-3-phosphate:ubiquinone oxidoreductase activity
GO:0052591 - sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity
biological_process GO:0009061 - anaerobic respiration
GO:0046174 - polyol catabolic process
GO:0006072 - glycerol-3-phosphate metabolic process
GO:0019563 - glycerol catabolic process
GO:0046168 - glycerol-3-phosphate catabolic process
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
Reference(s): [1] Ehrmann M., et al., 1987
[2] Freedberg WB., et al., 1973
[3] Iuchi S., et al., 1990
[4] Miki K., et al., 1979
[5] Schryvers A., et al., 1982
External database links:  
ECOCYC:
 ANGLYC3PDEHYDROGSUBUNITA-MONOMER
ECOLIWIKI:
 b2241
INTERPRO:
 IPR017752
INTERPRO:
 IPR036188
INTERPRO:
 IPR007419
INTERPRO:
 IPR006076
INTERPRO:
 IPR041854
INTERPRO:
 IPR000447
MODBASE:
 P0A9C0
PANTHER:
 PTHR11985
PFAM:
 PF04324
PFAM:
 PF01266
PRIDE:
 P0A9C0
PRINTS:
 PR01001
PRODB:
 PRO_000022792
PROSITE:
 PS00978
PROSITE:
 PS00977
REFSEQ:
 NP_416744
SMR:
 P0A9C0
UNIPROT:
 P0A9C0


Operon      
Name: glpABC         
Operon arrangement:
Transcription unit        Promoter
glpABC


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FNR, FlhDC, Fis
Repressed by: ArcA, GlpR


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yfaDp5 2356690 forward Similarity to the consensus
Read more > 		[ICWHO] [6]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Ehrmann M., Boos W., Ormseth E., Schweizer H., Larson TJ., 1987, Divergent transcription of the sn-glycerol-3-phosphate active transport (glpT) and anaerobic sn-glycerol-3-phosphate dehydrogenase (glpA glpC glpB) genes of Escherichia coli K-12., J Bacteriol 169(2):526-32

 [2] Freedberg WB., Lin EC., 1973, Three kinds of controls affecting the expression of the glp regulon in Escherichia coli., J Bacteriol 115(3):816-23

 [3] Iuchi S., Cole ST., Lin EC., 1990, Multiple regulatory elements for the glpA operon encoding anaerobic glycerol-3-phosphate dehydrogenase and the glpD operon encoding aerobic glycerol-3-phosphate dehydrogenase in Escherichia coli: further characterization of respiratory control., J Bacteriol 172(1):179-84

 [4] Miki K., Silhavy TJ., Andrews KJ., 1979, Resolution of glpA and glpT loci into separate operons in Escherichia coli K-12 strains., J Bacteriol 138(1):268-9

 [5] Schryvers A., Weiner JH., 1982, The anaerobic sn-glycerol-3-phosphate dehydrogenase: cloning and expression of the glpA gene of Escherichia coli and identification of the glpA products., Can J Biochem 60(3):224-31

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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