RegulonDB RegulonDB 10.6.3: Gene Form
   

glpT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glpA glpT glpQ glpB GlpR Fis FNR GlpR CRP Fis GlpR FNR CRP GlpR Fis Fis GlpR CRP Fis IHF Fis IHF GlpR GlpR GlpR terminator glpABCp glpABCp glpTQp glpTQp

Gene      
Name: glpT    Texpresso search in the literature
Synonym(s): ECK2232, EG10401, b2240
Genome position(nucleotides): 2351016 <-- 2352374 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.61
External database links:  
ASAP:
ABE-0007401
CGSC:
689
ECHOBASE:
EB0396
ECOCYC:
EG10401
ECOLIHUB:
glpT
OU-MICROARRAY:
b2240
REGULONDB:
b2240
STRING:
511145.b2240
M3D: glpT
COLOMBOS: glpT
PortEco: b2240


Product      
Name: sn-glycerol 3-phosphate:phosphate antiporter
Synonym(s): GlpT, GlpT glycerol-3-P MFS transporter
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 50.31
Isoelectric point: 8.58
Motif(s):
 
Type Positions Sequence
161 -> 180 WNCAHNVGGGIPPLLFLLGM
190 -> 207 LYMPAFCAILVALFAFAM
322 -> 341 GATGVFFMTLVTIATIVYWM
388 -> 409 TGLFGYLGGSVAASAIVGYTVD
37 -> 57 GYAAYYLVRKNFALAMPYLVE

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.6 - misc. glycerol metabolism
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015169 - glycerol-3-phosphate transmembrane transporter activity
GO:0015315 - organophosphate:inorganic phosphate antiporter activity
GO:0015527 - glycerol-phosphate:inorganic phosphate antiporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0006071 - glycerol metabolic process
GO:0006810 - transport
GO:0015793 - glycerol transport
GO:0015794 - glycerol-3-phosphate transmembrane transport
GO:0055085 - transmembrane transport
GO:0035435 - phosphate ion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Argast M., et al., 1978
[2] Boos W., et al., 1977
[3] Ehrmann M., et al., 1987
[4] Eiglmeier K., et al., 1987
[5] Fann MC., et al., 2003
[6] Freedberg WB., et al., 1973
[7] Kadner RJ., et al., 1992
[8] Larson TJ., et al., 1982
[9] Larson TJ., et al., 1987
[10] Ludtke D., et al., 1982
[11] Miki K., et al., 1980
[12] Miki K., et al., 1979
[13] Nilsson AI., et al., 2003
[14] Schryvers A., et al., 1982
[15] Schumacher G., et al., 1978
[16] Silhavy TJ., et al., 1976
[17] Yamada M., et al., 1982
External database links:  
ECOCYC:
GLPT-MONOMER
ECOLIWIKI:
b2240
INTERPRO:
IPR021159
INTERPRO:
IPR036259
INTERPRO:
IPR020846
INTERPRO:
IPR011701
INTERPRO:
IPR005267
INTERPRO:
IPR000849
MODBASE:
P08194
PANTHER:
PTHR43826:SF6
PDB:
1PW4
PFAM:
PF07690
PRIDE:
P08194
PROSITE:
PS00942
PROSITE:
PS50850
PROTEINMODELPORTAL:
P08194
REFSEQ:
NP_416743
SMR:
P08194
UNIPROT:
P08194


Operon      
Name: glpTQ         
Operon arrangement:
Transcription unit        Promoter
glpTQ


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FNR, Fis
Repressed by: IHF, GlpR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Argast M., Ludtke D., Silhavy TJ., Boos W., 1978, A second transport system for sn-glycerol-3-phosphate in Escherichia coli., J Bacteriol 136(3):1070-83

 [2] Boos W., Hartig-Beecken I., Altendorf K., 1977, Purification and properties of a periplasmic protein related to sn-glycerol-3-phosphate transport in Escherichia coli., Eur J Biochem 72(3):571-81

 [3] Ehrmann M., Boos W., Ormseth E., Schweizer H., Larson TJ., 1987, Divergent transcription of the sn-glycerol-3-phosphate active transport (glpT) and anaerobic sn-glycerol-3-phosphate dehydrogenase (glpA glpC glpB) genes of Escherichia coli K-12., J Bacteriol 169(2):526-32

 [4] Eiglmeier K., Boos W., Cole ST., 1987, Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system., Mol Microbiol 1(3):251-8

 [5] Fann MC., Busch A., Maloney PC., 2003, Functional characterization of cysteine residues in GlpT, the glycerol 3-phosphate transporter of Escherichia coli., J Bacteriol 185(13):3863-70

 [6] Freedberg WB., Lin EC., 1973, Three kinds of controls affecting the expression of the glp regulon in Escherichia coli., J Bacteriol 115(3):816-23

 [7] Kadner RJ., Murphy GP., Stephens CM., 1992, Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli., J Gen Microbiol 138(10):2007-14

 [8] Larson TJ., Schumacher G., Boos W., 1982, Identification of the glpT-encoded sn-glycerol-3-phosphate permease of Escherichia coli, an oligomeric integral membrane protein., J Bacteriol 152(3):1008-21

 [9] Larson TJ., Ye SZ., Weissenborn DL., Hoffmann HJ., Schweizer H., 1987, Purification and characterization of the repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K12., J Biol Chem 262(33):15869-74

 [10] Ludtke D., Larson TJ., Beck C., Boos W., 1982, Only one gene is required for the glpT-dependent transport of sn-glycerol-3-phosphate in Escherichia coli., Mol Gen Genet 186(4):540-7

 [11] Miki K., Lin EC., 1980, Use of Escherichia coli operon-fusion strains for the study of glycerol 3-phosphate transport activity., J Bacteriol 143(3):1436-43

 [12] Miki K., Silhavy TJ., Andrews KJ., 1979, Resolution of glpA and glpT loci into separate operons in Escherichia coli K-12 strains., J Bacteriol 138(1):268-9

 [13] Nilsson AI., Berg OG., Aspevall O., Kahlmeter G., Andersson DI., 2003, Biological costs and mechanisms of fosfomycin resistance in Escherichia coli., Antimicrob Agents Chemother 47(9):2850-8

 [14] Schryvers A., Weiner JH., 1982, The anaerobic sn-glycerol-3-phosphate dehydrogenase: cloning and expression of the glpA gene of Escherichia coli and identification of the glpA products., Can J Biochem 60(3):224-31

 [15] Schumacher G., Bussmann K., 1978, Cell-free synthesis of proteins related to sn-glycerol-3-phosphate transport in Escherichia coli., J Bacteriol 135(1):239-50

 [16] Silhavy TJ., Hartig-Beecken I., Boos W., 1976, Periplasmic protein related to the sn-glycerol-3-phosphate transport system of Escherichia coli., J Bacteriol 126(2):951-8

 [17] Yamada M., Takeda Y., Okamoto K., Hirota Y., 1982, Physical map of the nrdA-nrdB-ftsB-glpT region of the chromosomal DNA of Escherichia coli., Gene 18(3):309-18


RegulonDB