RegulonDB RegulonDB 10.8: Gene Form
   

gltB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gltB gltD yhcC unknown FNR CRP IHF IHF Lrp Lrp Lrp Lrp GadE ArgR ArgR TSS_3549 TSS_3549 TSS_3548 TSS_3548 TSS_3547 TSS_3547 TSS_3546 (cluster) TSS_3546 (cluster) TSS_3545 TSS_3545 TSS_3544 TSS_3544 TSS_3543 (cluster) TSS_3543 (cluster) TSS_3542 (cluster) TSS_3542 (cluster) TSS_3541 TSS_3541 TSS_3540 TSS_3540 TSS_3539 TSS_3539 TSS_3538 TSS_3538 TSS_3537 TSS_3537 TSS_3536 TSS_3536 TSS_3535 TSS_3535 TSS_3534 TSS_3534 TSS_3533 (cluster) TSS_3533 (cluster) gltBp gltBp yhcCp8 yhcCp8 yhcCp6 yhcCp6

Gene      
Name: gltB    Texpresso search in the literature
Synonym(s): ECK3202, EG10403, aspB, b3212, ossB, psiQ
Genome position(nucleotides): 3354725 --> 3359185 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.89
External database links:  
ASAP:
 ABE-0010545
CGSC:
 686
ECHOBASE:
 EB0398
OU-MICROARRAY:
 b3212
PortEco:
 gltB
STRING:
 511145.b3212
COLOMBOS: gltB


Product      
Name: glutamate synthase subunit GltB
Synonym(s): AspB, GltB, OssB, PsiQ, glutamate synthase, large subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 163.296
Isoelectric point: 6.561
Motif(s):
 
Type Positions Sequence
1 -> 11 MLYDKSLERDN
109 -> 109 E
429 -> 429 N
12 -> 402 CGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIVEEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIARRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNETGSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWDGPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTR
189 -> 209 LCMPTDLPRFYLDLADLRLES

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.1 - glutamate
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0016638 - oxidoreductase activity, acting on the CH-NH2 group of donors
GO:0004355 - glutamate synthase (NADPH) activity
GO:0015930 - glutamate synthase activity
GO:0004354 - glutamate dehydrogenase (NADP+) activity
GO:0051536 - iron-sulfur cluster binding
GO:0051538 - 3 iron, 4 sulfur cluster binding
biological_process GO:0006537 - glutamate biosynthetic process
GO:0006541 - glutamine metabolic process
GO:0006807 - nitrogen compound metabolic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0019676 - ammonia assimilation cycle
GO:0055114 - oxidation-reduction process
GO:0097054 - L-glutamate biosynthetic process
External database links:  
DIP:
 DIP-9802N
ECOCYC:
 GLUSYNLARGE-MONOMER
ECOLIWIKI:
 b3212
INTERPRO:
 IPR006982
INTERPRO:
 IPR036485
INTERPRO:
 IPR029055
INTERPRO:
 IPR017932
INTERPRO:
 IPR013785
INTERPRO:
 IPR002932
INTERPRO:
 IPR002489
MINT:
 MINT-1248755
MODBASE:
 P09831
PFAM:
 PF01493
PFAM:
 PF01645
PFAM:
 PF00310
PFAM:
 PF04898
PRIDE:
 P09831
PRODB:
 PRO_000022804
PROSITE:
 PS51278
REFSEQ:
 NP_417679
SMR:
 P09831
UNIPROT:
 P09831


Operon      
Name: gltBDF         
Operon arrangement:
Transcription unit        Promoter
gltBDF
gltF


Transcriptional Regulation      
Display Regulation             
Activated by: HdfR, IHF, Lrp, AdiY, GadE
Repressed by: CRP, Nac, FNR, Fur, ArgR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yhcCp6 3354218 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter yhcCp8 3354235 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_3533 (cluster) 3354511 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_3534 3356105 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3535 3356236 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3536 3356239 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3537 3356669 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3538 3356672 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3539 3356806 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3540 3357175 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3541 3357512 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3542 (cluster) 3357958 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_3543 (cluster) 3357974 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_3544 3357991 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3545 3359147 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3546 (cluster) 3359164 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_3547 3359356 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3548 3359426 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3549 3359434 forward nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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