RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

hemA gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	hemA
Synonym(s):	ECK1198, EG10427, b1210, gtrA
Genome position(nucleotides):	1263714 --> 1264970 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.34
Reference(s):	[1] Verkamp E., et al., 1989
External database links:

ASAP:	ABE-0004064
CGSC:	648
ECHOBASE:	EB0422
OU-MICROARRAY:	b1210
PortEco:	hemA
STRING:	511145.b1210
COLOMBOS:	hemA

Product

Name:

glutamyl-tRNA reductase

Synonym(s):

GtrA, HemA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

46.307

Isoelectric point:

5.359

Motif(s):

Type	Positions	Sequence
	50 -> 50	C
	52 -> 52	R
	114 -> 114	E
	145 -> 145	S
	170 -> 170	C

Type	Positions	Sequence
	50 -> 50	C
	52 -> 52	R
	114 -> 114	E
	145 -> 145	S
	170 -> 170	C
	49 -> 49	T
	74 -> 74	C
	99 -> 99	H
	240 -> 240	L
	6 -> 156	LGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGGVVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMRVASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTET
	106 -> 106	G
	7 -> 7	G
	54 -> 54	E
	109 -> 109	S
	151 -> 168	RVRTETDIGASAVSVAFA
	314 -> 314	R
	320 -> 416	EAETIVAQETSEFMAWLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAPTKSLQQAARDGDNERLNILRDSLG
	365 -> 365	A
	116 -> 116	Q
	189 -> 194	GAGETI
	191 -> 191	G
	172 -> 172	L
	243 -> 243	A

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.12 - heme, porphyrine
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0016491 - oxidoreductase activity GO:0008883 - glutamyl-tRNA reductase activity GO:0042802 - identical protein binding GO:0050661 - NADP binding
	biological_process	GO:0006779 - porphyrin-containing compound biosynthetic process GO:0033014 - tetrapyrrole biosynthetic process GO:0055114 - oxidation-reduction process GO:0019353 - protoporphyrinogen IX biosynthetic process from glutamate GO:0006782 - protoporphyrinogen IX biosynthetic process

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

GLUTRNAREDUCT-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

hemA-prfA-prmC

Operon arrangement:

Transcription unit	Promoter
hemA	hemAp2
hemA-prfA-prmC	hemAp1

Transcriptional Regulation
	Display Regulation
Activated by:	IHF, ArcA
Repressed by:	FNR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1541 (cluster)	1263536		reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1263535 to 1263536, where the highest frequency is at position 1262758. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
	promoter	TSS_1542	1263675		forward	nd	[RS-EPT-CBR]	[2]
	promoter	TSS_1543 (cluster)	1264894		forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1264894 to 1264895, where the highest frequency is at position 1264118. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
	promoter	TSS_1544 (cluster)	1264899		forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1264899 to 1264900, where the highest frequency is at position 1264123. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
	promoter	TSS_1545	1265281		forward	nd	[RS-EPT-CBR]	[2]
	promoter	TSS_1546	1265487		forward	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)

[1] Verkamp E., Chelm BK., 1989, Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene., J Bacteriol 171(9):4728-35

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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