RegulonDB RegulonDB 10.8: Gene Form
   

hemB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hemB tauD ykiB ytiB tauC hemBp7 hemBp7 hemBp hemBp

Gene      
Name: hemB    Texpresso search in the literature
Synonym(s): ECK0366, EG10428, b0369, ncf
Genome position(nucleotides): 388753 <-- 389727 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.95
External database links:  
ASAP:
ABE-0001266
CGSC:
647
ECHOBASE:
EB0423
MIM:
125270
MIM:
612740
OU-MICROARRAY:
b0369
PortEco:
hemB
STRING:
511145.b0369
COLOMBOS: hemB


Product      
Name: porphobilinogen synthase
Synonym(s): HemB, Ncf
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 35.625
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence
18 -> 19 RA
19 -> 42 AMFEETTLSLNDLVLPIFVEEEID
229 -> 229 A
231 -> 231 R
226 -> 226 R

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.12 - heme, porphyrine
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
GO:0004655 - porphobilinogen synthase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0006779 - porphyrin-containing compound biosynthetic process
GO:0033014 - tetrapyrrole biosynthetic process
GO:0006783 - heme biosynthetic process
GO:0006782 - protoporphyrinogen IX biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Adler H., et al., 1992
[2] Brana H., et al., 1977
[3] Cheung KM., et al., 1997
[4] Echelard Y., et al., 1988
[5] Erskine PT., et al., 1997
[6] Lewis LA., et al., 1994
[7] Lewis LA., et al., 1994
[8] Li F., et al., 2014
[9] Li JM., et al., 1989
[10] Li JM., et al., 1988
[11] Lin J., et al., 2009
[12] Norton E., et al., 1998
[13] O'Neill GP., et al., 1991
[14] Philipp-Dormston WK., et al., 1975
[15] Shimoni-Livny L., et al., 1998
[16] Spencer P., et al., 1994
External database links:  
DIP:
DIP-36209N
ECOCYC:
PORPHOBILSYNTH-MONOMER
ECOLIWIKI:
b0369
INTERPRO:
IPR030656
INTERPRO:
IPR013785
INTERPRO:
IPR001731
MINT:
P0ACB2
MODBASE:
P0ACB2
PANTHER:
PTHR11458
PDB:
5MHB
PDB:
1L6Y
PDB:
1L6S
PDB:
1B4E
PDB:
1I8J
PFAM:
PF00490
PRIDE:
P0ACB2
PRINTS:
PR00144
PROSITE:
PS00169
REFSEQ:
NP_414903
SMART:
SM01004
SMR:
P0ACB2
UNIPROT:
P0ACB2


Operon      
Name: hemB         
Operon arrangement:
Transcription unit        Promoter
hemB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter hemBp7 389861 reverse Similarity to the consensus
Read more >
[ICWHO] [17]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Adler H., Mural R., Suttle B., 1992, Oxygen sensitivity of an Escherichia coli mutant., J Bacteriol 174(7):2072-7

 [2] Brana H., Hubacek J., Michaljanicova D., Holubova I., Cejka K., 1977, Membrane mutation affecting energy-linked functions in Escherichia coli K 12., Folia Microbiol (Praha) 22(3):198-205

 [3] Cheung KM., Spencer P., Timko MP., Shoolingin-Jordan PM., 1997, Characterization of a recombinant pea 5-aminolevulinic acid dehydratase and comparative inhibition studies with the Escherichia coli dehydratase., Biochemistry 36(5):1148-56

 [4] Echelard Y., Dymetryszyn J., Drolet M., Sasarman A., 1988, Nucleotide sequence of the hemB gene of Escherichia coli K12., Mol Gen Genet 214(3):503-8

 [5] Erskine PT., Senior N., Maignan S., Cooper J., Lambert R., Lewis G., Spencer P., Awan S., Warren M., Tickle IJ., Thomas P., Wood SP., Shoolingin-Jordan PM., 1997, Crystallization of 5-aminolaevulinic acid dehydratase from Escherichia coli and Saccharomyces cerevisiae and preliminary X-ray characterization of the crystals., Protein Sci 6(8):1774-6

 [6] Lewis LA., Gopaul S., Marsh C., 1994, The non-random pattern of insertion of IS2 into the hemB gene of Escherichia coli., Microbiol Immunol 38(6):461-5

 [7] Lewis LA., Lewis D., Persaud V., Gopaul S., Turner B., 1994, Transposition of IS2 into the hemB gene of Escherichia coli K-12., J Bacteriol 176(7):2114-20

 [8] Li F., Wang Y., Gong K., Wang Q., Liang Q., Qi Q., 2014, Constitutive expression of RyhB regulates the heme biosynthesis pathway and increases the 5-aminolevulinic acid accumulation in Escherichia coli., FEMS Microbiol Lett 350(2):209-15

 [9] Li JM., Russell CS., Cosloy SD., 1989, The structure of the Escherichia coli hemB gene., Gene 75(1):177-84

 [10] Li JM., Umanoff H., Proenca R., Russell CS., Cosloy SD., 1988, Cloning of the Escherichia coli K-12 hemB gene., J Bacteriol 170(2):1021-5

 [11] Lin J., Fu W., Cen P., 2009, Characterization of 5-aminolevulinate synthase from Agrobacterium radiobacter, screening new inhibitors for 5-aminolevulinate dehydratase from Escherichia coli and their potential use for high 5-aminolevulinate production., Bioresour Technol 100(7):2293-7

 [12] Norton E., Sarwar M., Shoolingin-Jordan P., 1998, Mechanistic studies on E.coli 5-aminolaevulinic acid dehydratase., Biochem Soc Trans 26(3):S285

 [13] O'Neill GP., Thorbjarnardottir S., Michelsen U., Palsson S., Soll D., Eggertsson G., 1991, delta-Aminolevulinic acid dehydratase deficiency can cause delta-aminolevulinate auxotrophy in Escherichia coli., J Bacteriol 173(1):94-100

 [14] Philipp-Dormston WK., Doss M., 1975, Over-production of porphyrins and heme in heterotrophic bacteria., Z Naturforsch C 30(3):425-6

 [15] Shimoni-Livny L., Carrell HL., Wagner T., Kaufman Katz A., Afshar C., Mitchell LW., Volin M., Jaffe EK., Glusker JP., 1998, Crystallization and preliminary X-ray diffraction studies of E. coli porphobilinogen synthase and its heavy-atom derivatives., Acta Crystallogr D Biol Crystallogr 54(Pt 3):438-40

 [16] Spencer P., Jordan PM., 1994, 5-Aminolaevulinic acid dehydratase: characterization of the alpha and beta metal-binding sites of the Escherichia coli enzyme., Ciba Found Symp 180:50-64: discussion 64-9

 [17] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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