RegulonDB RegulonDB 11.1: Gene Form
   

hyaB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hyaB hyaA hyaC serT hyaD NarL NarP NarL ArcA NarP Fis IscR IscR YdeO ArcA Fis anti-anti-terminator anti-terminator hyaAp2 hyaAp2 hyaAp hyaAp serTp serTp

Gene      
Name: hyaB    Texpresso search in the literature
Synonym(s): ECK0964, EG10469, b0973
Genome position(nucleotides): 1033254 --> 1035047
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.52
External database links:  
ASAP:
 ABE-0003288
CGSC:
 31784
ECHOBASE:
 EB0464
ECOLIHUB:
 hyaB
OU-MICROARRAY:
 b0973
STRING:
 511145.b0973
COLOMBOS: hyaB


Shine dalgarno      
Sequence: gccaggcaatGAGGATaaacaggcATG


Product      
Name: hydrogenase 1 large subunit
Synonym(s): HyaB, hydrogenase 1 alpha; subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 66.253
Isoelectric point: 5.842
Motif(s):
 
Type Positions Sequence Comment
28 -> 28 E E → Q: loss of Hyd-1 catalytic activity
52 -> 52 M UniProt: In Ref. 1; AAA23998..
54 -> 582 RGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTH
69 -> 69 W UniProt: In Ref. 1; AAA23998..
193 -> 193 K R → L: altered catalytic bias of Hyd-1 towards H2 production

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
Gene Ontology Terms (GO)  
cellular_component GO:0098567 - periplasmic side of plasma membrane
GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0044569 - [Ni-Fe] hydrogenase complex
molecular_function GO:0005515 - protein binding
GO:0009055 - electron transfer activity
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0016151 - nickel cation binding
GO:0008901 - ferredoxin hydrogenase activity
GO:0033748 - hydrogenase (acceptor) activity
biological_process GO:0006113 - fermentation
GO:0009061 - anaerobic respiration
GO:0009267 - cellular response to starvation
GO:0019645 - anaerobic electron transport chain
GO:1902421 - hydrogen metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Evans RM., et al., 2018
[2] Evans RM., et al., 2021
[3] Przybyla AE., et al., 1992
External database links:  
ALPHAFOLD:
 P0ACD8
DIP:
 DIP-36180N
ECOCYC:
 HYAB-MONOMER
ECOLIWIKI:
 b0973
INTERPRO:
 IPR029014
INTERPRO:
 IPR018194
INTERPRO:
 IPR001501
MODBASE:
 P0ACD8
PDB:
 5JRD
PDB:
 5LMM
PDB:
 5LRY
PDB:
 6FPI
PDB:
 6FPO
PDB:
 6FPW
PDB:
 6G7R
PDB:
 6G94
PDB:
 6GAL
PDB:
 6RP2
PDB:
 5ADU
PDB:
 5A4M
PDB:
 5A4I
PDB:
 5A4F
PDB:
 4UE3
PDB:
 4GD3
PDB:
 3USE
PDB:
 3USC
PDB:
 3UQY
PFAM:
 PF00374
PRIDE:
 P0ACD8
PRODB:
 PRO_000022944
PROSITE:
 PS00507
PROSITE:
 PS00508
REFSEQ:
 NP_415492
SMR:
 P0ACD8
UNIPROT:
 P0ACD8


Operon      
Name: hyaABCDEF         
Operon arrangement:
Transcription unit        Promoter
hyaABCDEF
hyaABCDEF


Transcriptional Regulation      
Display Regulation             
Activated by: ArcA, YdeO, AppY
Repressed by: IscR, NarL, NarP, Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Evans RM., Ash PA., Beaton SE., Brooke EJ., Vincent KA., Carr SB., Armstrong FA., 2018, Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O2 -Tolerant [NiFe]-Hydrogenase., J Am Chem Soc 140(32):10208-10220

 [2] Evans RM., Krahn N., Murphy BJ., Lee H., Armstrong FA., Soll D., 2021, Selective cysteine-to-selenocysteine changes in a [NiFe]-hydrogenase confirm a special position for catalysis and oxygen tolerance., Proc Natl Acad Sci U S A 118(13)

 [3] Przybyla AE., Robbins J., Menon N., Peck HD., 1992, Structure-function relationships among the nickel-containing hydrogenases., FEMS Microbiol Rev 8(2):109-35

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