RegulonDB RegulonDB 10.7: Gene Form
   

ileS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ileS ribF lspA fkpB lspAp lspAp TSS_72 TSS_72 TSS_71 TSS_71 TSS_70 TSS_70 TSS_69 TSS_69 TSS_68 TSS_68 TSS_67 (cluster) TSS_67 (cluster) TSS_66 (cluster) TSS_66 (cluster) TSS_65 (cluster) TSS_65 (cluster) TSS_64 TSS_64 TSS_63 TSS_63 TSS_62 TSS_62 ileSp3 ileSp3 TSS_60 TSS_60 TSS_59 TSS_59 ileSp2 ileSp2 TSS_58 (cluster) TSS_58 (cluster) ileSp1 ileSp1

Gene      
Name: ileS    Texpresso search in the literature
Synonym(s): ECK0027, EG10492, b0026, ilvS
Genome position(nucleotides): 22391 --> 25207 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.77
External database links:  
ASAP:
ABE-0000094
CGSC:
613
ECHOBASE:
EB0487
MIM:
616007
OU-MICROARRAY:
b0026
PORTECO:
ileS
REGULONDB:
b0026
STRING:
511145.b0026
M3D: ileS
COLOMBOS: ileS
PortEco: b0026


Product      
Name: isoleucine—tRNA ligase
Synonym(s): IleRS, IleS, IlvS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 104.297
Isoelectric point: 5.868
Motif(s):
 
Type Positions Sequence
637 -> 637 E
246 -> 246 T
587 -> 587 R
602 -> 606 KMSKS
867 -> 869 GAT

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0004822 - isoleucine-tRNA ligase activity
GO:0000049 - tRNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0002161 - aminoacyl-tRNA editing activity
biological_process GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity
GO:0006412 - translation
GO:0006418 - tRNA aminoacylation for protein translation
GO:0006428 - isoleucyl-tRNA aminoacylation
GO:0046677 - response to antibiotic
Note(s): Note(s): ...[more].
Reference(s): [1] Baldwin AN., et al., 1966
[2] Goh S., et al., 2015
[3] Jakubowski H. 1995
[4] Shepard A., et al., 1992
[5] Webster T., et al., 1984
External database links:  
DIP:
DIP-10017N
ECOCYC:
ILES-MONOMER
ECOLIWIKI:
b0026
INTERPRO:
IPR002300
INTERPRO:
IPR014729
INTERPRO:
IPR033708
INTERPRO:
IPR001412
INTERPRO:
IPR023585
INTERPRO:
IPR002301
INTERPRO:
IPR009008
INTERPRO:
IPR009080
INTERPRO:
IPR010663
INTERPRO:
IPR013155
MINT:
MINT-1232480
MODBASE:
P00956
PANTHER:
PTHR11946:SF9
PFAM:
PF00133
PFAM:
PF08264
PFAM:
PF06827
PRIDE:
P00956
PRINTS:
PR00984
PRODB:
PRO_000023000
PROSITE:
PS00178
PROTEINMODELPORTAL:
P00956
REFSEQ:
NP_414567
SMR:
P00956
UNIPROT:
P00956


Operon      
Name: ribF-ileS-lspA-fkpB-ispH         
Operon arrangement:
Transcription unit        Promoter
ribF-ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
lsp-fkpB-ispH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_58 (cluster) 21834 forward For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_59 22220 forward nd [RS-EPT-CBR] [6]
  promoter TSS_60 22222 forward nd [RS-EPT-CBR] [6]
  promoter TSS_62 22244 forward nd [RS-EPT-CBR] [6]
  promoter TSS_63 22262 forward nd [RS-EPT-CBR] [6]
  promoter TSS_64 22279 forward nd [RS-EPT-CBR] [6]
  promoter TSS_65 (cluster) 22306 forward For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_66 (cluster) 22371 forward For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_67 (cluster) 23007 forward For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_68 23888 forward nd [RS-EPT-CBR] [6]
  promoter TSS_69 24198 forward nd [RS-EPT-CBR] [6]
  promoter TSS_70 24243 forward nd [RS-EPT-CBR] [6]
  promoter TSS_71 24709 forward nd [RS-EPT-CBR] [6]
  promoter TSS_72 24918 forward nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Baldwin AN., Berg P., 1966, Transfer ribonucleic acid-induced hydrolysis of valyladenylate bound to isoleucyl ribonucleic acid synthetase., J Biol Chem 241(4):839-45

 [2] Goh S., Hohmeier A., Stone TC., Offord V., Sarabia F., Garcia-Ruiz C., Good L., 2015, Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli., Appl Environ Microbiol 81(16):5650-9

 [3] Jakubowski H., 1995, Proofreading in vivo. Editing of homocysteine by aminoacyl-tRNA synthetases in Escherichia coli., J Biol Chem 270(30):17672-3

 [4] Shepard A., Shiba K., Schimmel P., 1992, RNA binding determinant in some class I tRNA synthetases identified by alignment-guided mutagenesis., Proc Natl Acad Sci U S A 89(20):9964-8

 [5] Webster T., Tsai H., Kula M., Mackie GA., Schimmel P., 1984, Specific sequence homology and three-dimensional structure of an aminoacyl transfer RNA synthetase., Science 226(4680):1315-7

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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