RegulonDB

Gene
Name:	katE
Synonym(s):	ECK1730, EG10509, b1732
Genome position(nucleotides):	1813867 --> 1816128 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.08
External database links:

ASAP:	ABE-0005780
CGSC:	593
ECHOBASE:	EB0504
MIM:	614097
OU-MICROARRAY:	b1732
PortEco:	katE
STRING:	511145.b1732
COLOMBOS:	katE

Product

Name:

catalase HPII

Synonym(s):

KatE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

84.162

Isoelectric point:

5.728

Motif(s):

Type	Positions	Sequence
	599 -> 746	KGRVVAILLNDEVRSADLLAILKALKAKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHRVWSRIP
	81 -> 466	TTNQGVRIADDQNSLRAGSRGPTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKITPVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFVHAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHTFRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEYELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFHPGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHRMGIDTNPANYEPNSIN
	499 -> 562	PSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNL

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.6 - protection --> 5.6.2 - detoxification
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0046872 - metal ion binding GO:0016491 - oxidoreductase activity GO:0004601 - peroxidase activity GO:0004096 - catalase activity GO:0005506 - iron ion binding GO:0020037 - heme binding GO:0042802 - identical protein binding
	biological_process	GO:0006974 - cellular response to DNA damage stimulus GO:0006979 - response to oxidative stress GO:0006972 - hyperosmotic response GO:0042744 - hydrogen peroxide catabolic process GO:0055114 - oxidation-reduction process GO:0098869 - cellular oxidant detoxification

Note(s):

Note(s): ...[more].

Reference(s):

[1] Benov L., et al., 2003
[2] Hoerter JD., et al., 2005
[3] Jung IL., et al., 2003
[4] Loewen PC., et al., 1984
[5] Mulvey MR., et al., 1988
[6] Mulvey MR., et al., 1990
[7] Oktyabrsky ON., et al., 2001
[8] Park C., et al., 2007
[9] Peng Q., et al., 1992
[10] Schellhorn HE., et al., 1992
[11] Semchyshyn HM., et al., null
[12] Semchyshyn HM., et al., null
[13] Smirnova GV., et al., 2000
[14] Smirnova GV., et al., null

External database links:

DIP:

DIP-10052N

ECOCYC:

HYDROPEROXIDII-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

katE

Operon arrangement:

Transcription unit	Promoter
katE	katEp

Transcriptional Regulation

Display Regulation

Activated by:

Fur

Repressed by:

Fis

Growth Conditions:

[1]	C: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| oxygen atom\| exponential phase E: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| exponential phase
[2]	C: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL E: Escherichia coli\| rpoS knockout mutant\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL
[3]	C: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| oxygen atom E: Escherichia coli\| rpoS knockout mutant\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| oxygen atom
[4]	C: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| oxygen atom\| exponential phase E: Escherichia coli\| minimal medium\| casamino acids 0.2%; glucose 0.4%; thiamine 0.2 µg/mL\| oxygen atom\| stationary phase

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Benov L., Sequeira F., 2003, Escherichia coli deltafur mutant displays low HPII catalase activity in stationary phase., Redox Rep 8(6):379-83
[2] Hoerter JD., Arnold AA., Kuczynska DA., Shibuya A., Ward CS., Sauer MG., Gizachew A., Hotchkiss TM., Fleming TJ., Johnson S., 2005, Effects of sublethal UVA irradiation on activity levels of oxidative defense enzymes and protein oxidation in Escherichia coli., J Photochem Photobiol B 81(3):171-80
[3] Jung IL., Kim IG., 2003, Transcription of ahpC, katG, and katE genes in Escherichia coli is regulated by polyamines: polyamine-deficient mutant sensitive to H2O2-induced oxidative damage., Biochem Biophys Res Commun 301(4):915-22
[4] Loewen PC., Triggs BL., 1984, Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli., J Bacteriol 160(2):668-75
[5] Mulvey MR., Sorby PA., Triggs-Raine BL., Loewen PC., 1988, Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli., Gene 73(2):337-45
[6] Mulvey MR., Switala J., Borys A., Loewen PC., 1990, Regulation of transcription of katE and katF in Escherichia coli., J Bacteriol 172(12):6713-20
[7] Oktyabrsky ON., Smirnovam GV., Muzyka NG., 2001, Role of glutathione in regulation of hydroperoxidase I in growing Escherichia coli., Free Radic Biol Med 31(2):250-5
[8] Park C., Zhou S., Gilmore J., Marqusee S., 2007, Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis., J Mol Biol 368(5):1426-37
[9] Peng Q., Timkovich R., Loewen PC., Peterson J., 1992, Identification of heme macrocycle type by near-infrared magnetic circular dichroism spectroscopy at cryogenic temperatures., FEBS Lett 309(2):157-60
[10] Schellhorn HE., Stones VL., 1992, Regulation of katF and katE in Escherichia coli K-12 by weak acids., J Bacteriol 174(14):4769-76
[11] Semchyshyn HM., Dyl'ovyi MV., Lushchak VI., null, [pH-dependency of Escherichia coli catalase activity under modified culture conditions]., Ukr Biokhim Zh 74(5):34-41
[12] Semchyshyn HM., Lushchak VI., null, [Oxidative stress and control of catalase activity in Escherichia coli]., Ukr Biokhim Zh 76(2):31-42
[13] Smirnova GV., Muzyka NG., Oktyabrsky ON., 2000, The role of antioxidant enzymes in response of Escherichia coli to osmotic upshift., FEMS Microbiol Lett 186(2):209-13
[14] Smirnova GV., Zakirova ON., Oktiabr'skii ON., null, [The role of antioxidant systems in response of bacteria Escherichia coli to heat shock]., Mikrobiologiia 70(5):595-601