RegulonDB RegulonDB 10.8: Gene Form
   

lpxB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dnaE lpxB lpxA rnhB fabZ FadR accAp accAp accAp2 accAp2 TSS_386 TSS_386 TSS_385 TSS_385 TSS_384 TSS_384 TSS_383 TSS_383 TSS_382 TSS_382 TSS_381 TSS_381 TSS_380 TSS_380 TSS_379 TSS_379 TSS_378 TSS_378 TSS_377 TSS_377 TSS_376 TSS_376 TSS_375 TSS_375

Gene      
Name: lpxB    Texpresso search in the literature
Synonym(s): ECK0181, EG10546, b0182, pgsB
Genome position(nucleotides): 203348 --> 204496 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.79
External database links:  
ASAP:
ABE-0000620
CGSC:
404
ECHOBASE:
EB0541
OU-MICROARRAY:
b0182
PortEco:
lpxB
STRING:
511145.b0182
COLOMBOS: lpxB


Product      
Name: lipid A disaccharide synthase
Synonym(s): LpxB, PgsB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 42.382
Isoelectric point: 7.044
Motif(s):
 
Type Positions Sequence
149 -> 149 F
9 -> 377 IALVAGETSGDILGAGLIRALKEHVPNARFVGVAGPRMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDFNITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVPCRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQLLRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASGTAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEPQKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAV

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.3 - lipid A
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0019897 - extrinsic component of plasma membrane
GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane
molecular_function GO:0005515 - protein binding
GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0008915 - lipid-A-disaccharide synthase activity
GO:0042802 - identical protein binding
GO:0005543 - phospholipid binding
biological_process GO:0006629 - lipid metabolic process
GO:0009245 - lipid A biosynthetic process
External database links:  
CAZY:
GT19
DIP:
DIP-10122N
ECOCYC:
LIPIDADISACCHARIDESYNTH-MONOMER
ECOLIWIKI:
b0182
INTERPRO:
IPR003835
MINT:
MINT-1246245
MODBASE:
P10441
PANTHER:
PTHR30372
PDB:
5W8S
PDB:
5W8X
PDB:
5W8N
PFAM:
PF02684
PRIDE:
P10441
PRODB:
PRO_000023121
REFSEQ:
NP_414724
SMR:
P10441
UNIPROT:
P10441


Operon      
Name: bamA-skp-lpxD-fabZ-lpxAB-rnhB-dnaE         
Operon arrangement:
Transcription unit        Promoter
bamA-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE
bamA
hlpA-lpxD-fabZ-lpxA
lpxD
fabZ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_375 202110 forward nd [RS-EPT-CBR] [1]
  promoter TSS_376 202370 forward nd [RS-EPT-CBR] [1]
  promoter TSS_377 202372 forward nd [RS-EPT-CBR] [1]
  promoter TSS_378 202585 forward nd [RS-EPT-CBR] [1]
  promoter TSS_379 202645 forward nd [RS-EPT-CBR] [1]
  promoter TSS_380 204501 forward nd [RS-EPT-CBR] [1]
  promoter TSS_381 204507 forward nd [RS-EPT-CBR] [1]
  promoter TSS_382 206909 forward nd [RS-EPT-CBR] [1]
  promoter TSS_383 207634 forward nd [RS-EPT-CBR] [1]
  promoter TSS_384 207925 forward nd [RS-EPT-CBR] [1]
  promoter TSS_385 208042 forward nd [RS-EPT-CBR] [1]
  promoter TSS_386 208276 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


RegulonDB