RegulonDB RegulonDB 10.8: Gene Form
   

mcrC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yjiV yjiT mcrB mcrC symE symR symRp symRp mcrCp5 mcrCp5 TSS_5148 TSS_5148

Gene      
Name: mcrC    Texpresso search in the literature
Synonym(s): ECK4335, EG10575, b4345
Genome position(nucleotides): 4576912 <-- 4577958 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
37.44
External database links:  
ASAP:
ABE-0014248
CGSC:
34613
ECHOBASE:
EB0570
OU-MICROARRAY:
b4345
PortEco:
mcrC
STRING:
511145.b4345
COLOMBOS: mcrC


Product      
Name: 5-methylcytosine-specific restriction enzyme subunit McrC
Synonym(s): McrC, MrcC subunit of 5-methylcytosine restriction system
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 40.59
Isoelectric point: 9.297
Motif(s):
 
Type Positions Sequence
13 -> 304 YMLTYAWGYLQEIKQANLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAKTIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAILIKHEKLNSTIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTRYYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPRMETDITIRSSEKILIVDAKYYKSIFSRRMGTEKFHSQNLYQLMNYLWSLKPENGENIGGLLIYP

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.2 - DNA
  2 - information transfer --> 2.1 - DNA related --> 2.1.2 - DNA restriction/modification
  2 - information transfer --> 2.1 - DNA related --> 2.1.5 - DNA degradation
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0032067 - type IV site-specific deoxyribonuclease activity
biological_process GO:0009307 - DNA restriction-modification system
Note(s): Note(s): ...[more].
Reference(s): [1] Gast FU., et al., 1997
[2] Nirwan N., et al., 2019
[3] Piekarowicz A., et al., 1991
[4] Pieper U., et al., 2002
[5] Wei Y., et al., 2001
[6] Yura T., et al., 2019
External database links:  
ECOCYC:
EG10575-MONOMER
ECOLIWIKI:
b4345
INTERPRO:
IPR019292
INTERPRO:
IPR014407
PANTHER:
PTHR38733
PDB:
6HZ8
PDB:
6HZ9
PDB:
6HZ7
PDB:
6HZ6
PDB:
6HZ5
PDB:
6HZ4
PFAM:
PF10117
PRIDE:
P15006
PRODB:
PRO_000023172
REFSEQ:
NP_418765
SMR:
P15006
UNIPROT:
P15006


Operon      
Name: mcrBC         
Operon arrangement:
Transcription unit        Promoter
mcrBC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5148 4574607 forward nd [RS-EPT-CBR] [7]
  promoter mcrCp5 4578062 reverse Similarity to the consensus
Read more >
[ICWHO] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Gast FU., Brinkmann T., Pieper U., Kruger T., Noyer-Weidner M., Pingoud A., 1997, The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB., Biol Chem 378(9):975-82

 [2] Nirwan N., Itoh Y., Singh P., Bandyopadhyay S., Vinothkumar KR., Amunts A., Saikrishnan K., 2019, Structure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC., Nat Commun 10(1):3058

 [3] Piekarowicz A., Yuan R., Stein DC., 1991, Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12., J Bacteriol 173(1):150-5

 [4] Pieper U., Pingoud A., 2002, A mutational analysis of the PD...D/EXK motif suggests that McrC harbors the catalytic center for DNA cleavage by the GTP-dependent restriction enzyme McrBC from Escherichia coli., Biochemistry 41(16):5236-44

 [5] Wei Y., Lee JM., Smulski DR., LaRossa RA., 2001, Global impact of sdiA amplification revealed by comprehensive gene expression profiling of Escherichia coli., J Bacteriol 183(7):2265-72

 [6] Yura T., Miyazaki R., Fujiwara K., Ito K., Chiba S., Mori H., Akiyama Y., 2019, Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli., Genes Genet Syst 93(6):229-235

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [8] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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