RegulonDB RegulonDB 10.6.3: Gene Form
   

mglB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mglA galS mglB GalR GalS GalS GalR CRP mglBp mglBp mglAp mglAp mglCp6 mglCp6 mglCp9 mglCp9 mglCp5 mglCp5

Gene      
Name: mglB    Texpresso search in the literature
Synonym(s): ECK2143, EG10593, b2150
Genome position(nucleotides): 2239350 <-- 2240348 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.05
External database links:  
ASAP:
ABE-0007108
CGSC:
501
ECHOBASE:
EB0588
ECOCYC:
EG10593
ECOLIHUB:
mglB
OU-MICROARRAY:
b2150
REGULONDB:
b2150
STRING:
511145.b2150
M3D: mglB
COLOMBOS: mglB
PortEco: b2150


Shine dalgarno      
Sequence: taagaaaaacCGGAGAtacCAT


Product      
Name: D-galactose/methyl-galactoside ABC transporter periplasmic binding protein
Synonym(s): D-galactose/D-glucose binding protein, GBP, GGBP, MglB
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 35.713
Isoelectric point: 5.779
Motif(s):
 
Type Positions Sequence
1 -> 23 MNKKVLTLSAVMASMLFGAAAHA
276 -> 279 TVLN
28 -> 299 IGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGK
24 -> 332 ADTRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK
227 -> 228 IE

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.p - ABC superfamily, periplasmic binding component
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0005534 - galactose binding
GO:0005536 - glucose binding
biological_process GO:0008643 - carbohydrate transport
GO:0015757 - galactose transmembrane transport
GO:0006935 - chemotaxis
GO:0015765 - methylgalactoside transport
Note(s): Note(s): ...[more].
Reference(s): [1] Anraku Y. 1968
[2] Boos W. 1972
[3] Boos W. 1969
[4] Boos W., et al., 1972
[5] D'Auria S., et al., null
[6] D'Auria S., et al., 2006
[7] D'Auria S., et al., 2007
[8] Hazelbauer GL., et al., 1969
[9] Herman P., et al., 2005
[10] Miller DM., et al., 1983
[11] Miller DM., et al., 1980
[12] Mowbray SL. 1992
[13] Muller N., et al., 1982
[14] Park C., et al., 1986
[15] Parsons RG., et al., 1974
[16] Quiocho FA., et al., 1979
[17] Richarme G. 1983
[18] Scholle A., et al., 1987
[19] Scognamiglio V., et al., 2007
[20] Shilton BH., et al., 1996
[21] Vyas NK., et al., 1991
External database links:  
DIP:
DIP-35953N
ECOCYC:
MGLB-MONOMER
ECOLIWIKI:
b2150
INTERPRO:
IPR028082
INTERPRO:
IPR025997
MINT:
MINT-1274707
MODBASE:
P0AEE5
PDB:
2GBP
PDB:
2IPN
PDB:
2QW1
PDB:
2IPM
PDB:
2IPL
PDB:
2HPH
PDB:
1GLG
PDB:
2FVY
PDB:
2FW0
PFAM:
PF13407
PRIDE:
P0AEE5
PRODB:
PRO_000023223
PROTEINMODELPORTAL:
P0AEE5
REFSEQ:
NP_416655
SMR:
P0AEE5
SWISSMODEL:
P0AEE5
UNIPROT:
P0AEE5


Operon      
Name: mglBAC         
Operon arrangement:
Transcription unit        Promoter
mglAC
mglBAC


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: GalS, FlhDC, GalR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mglCp5 2237858 reverse Similarity to the consensus
Read more >
[ICWHO] [22]
  promoter mglCp9 2237923 reverse Similarity to the consensus
Read more >
[ICWHO] [22]
  promoter mglCp6 2237959 reverse Similarity to the consensus
Read more >
[ICWHO] [22]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Anraku Y., 1968, Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins., J Biol Chem 243(11):3116-22

 [2] Boos W., 1972, Structurally defective galactose-binding protein isolated from a mutant negative in the -methylgalactoside transport system of Escherichia coli., J Biol Chem 247(17):5414-24

 [3] Boos W., 1969, The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli., Eur J Biochem 10(1):66-73

 [4] Boos W., Gordon AS., Hall RE., Price HD., 1972, Transport properties of the galactose-binding protein of Escherichia coli. Substrate-induced conformational change., J Biol Chem 247(3):917-24

 [5] D'Auria S., Alfieri F., Staiano M., Pelella F., Rossi M., Scire A., Tanfani F., Bertoli E., Grycznyski Z., Lakowicz JR., null, Structural and thermal stability characterization of Escherichia coli D-galactose/D-glucose-binding protein., Biotechnol Prog 20(1):330-7

 [6] D'Auria S., Ausili A., Marabotti A., Varriale A., Scognamiglio V., Staiano M., Bertoli E., Rossi M., Tanfani F., 2006, Binding of glucose to the D-galactose/D-glucose-binding protein from Escherichia coli restores the native protein secondary structure and thermostability that are lost upon calcium depletion., J Biochem 139(2):213-21

 [7] D'Auria S., Varriale A., Gonnelli M., Saviano M., Staiano M., Rossi M., Strambini GB., 2007, Tryptophan phosphorescence studies of the D-galactose/D-glucose-binding protein from Escherichia coli provide a molecular portrait with structural and dynamics features of the protein., J Proteome Res 6(4):1306-12

 [8] Hazelbauer GL., Mesibov RE., Adler J., 1969, Escherichia coli mutants defective in chemotaxis toward specific chemicals., Proc Natl Acad Sci U S A 64(4):1300-7

 [9] Herman P., Vecer J., Barvik I., Scognamiglio V., Staiano M., de Champdore M., Varriale A., Rossi M., D'Auria S., 2005, The role of calcium in the conformational dynamics and thermal stability of the D-galactose/D-glucose-binding protein from Escherichia coli., Proteins 61(1):184-95

 [10] Miller DM., Olson JS., Pflugrath JW., Quiocho FA., 1983, Rates of ligand binding to periplasmic proteins involved in bacterial transport and chemotaxis., J Biol Chem 258(22):13665-72

 [11] Miller DM., Olson JS., Quiocho FA., 1980, The mechanism of sugar binding to the periplasmic receptor for galactose chemotaxis and transport in Escherichia coli., J Biol Chem 255(6):2465-71

 [12] Mowbray SL., 1992, Ribose and glucose-galactose receptors. Competitors in bacterial chemotaxis., J Mol Biol 227(2):418-40

 [13] Muller N., Heine HG., Boos W., 1982, Cloning of mglB, the structural gene for the galactose-binding protein of Salmonella typhimurium and Escherichia coli., Mol Gen Genet 185(3):473-80

 [14] Park C., Hazelbauer GL., 1986, Mutations specifically affecting ligand interaction of the Trg chemosensory transducer., J Bacteriol 167(1):101-9

 [15] Parsons RG., Hogg RW., 1974, A comparison of the L-arabinose- and D-galactose-binding proteins of Escherichia coli B-r., J Biol Chem 249(11):3608-14

 [16] Quiocho FA., Meador WE., Pflugrath JW., 1979, Preliminary crystallographic data of receptors for transport and chemotaxis in Escherichia coli: D-galactose and maltose-binding proteins., J Mol Biol 133(1):181-4

 [17] Richarme G., 1983, Associative properties of the Escherichia coli galactose-binding protein and maltose-binding protein., Biochim Biophys Acta 748(1):99-108

 [18] Scholle A., Vreemann J., Blank V., Nold A., Boos W., Manson MD., 1987, Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors., Mol Gen Genet 208(1-2):247-53

 [19] Scognamiglio V., Scire A., Aurilia V., Staiano M., Crescenzo R., Palmucci C., Bertoli E., Rossi M., Tanfani F., D'Auria S., 2007, A strategic fluorescence labeling of D-galactose/D-glucose-binding protein from Escherichia coli helps to shed light on the protein structural stability and dynamics., J Proteome Res 6(11):4119-26

 [20] Shilton BH., Flocco MM., Nilsson M., Mowbray SL., 1996, Conformational changes of three periplasmic receptors for bacterial chemotaxis and transport: the maltose-, glucose/galactose- and ribose-binding proteins., J Mol Biol 264(2):350-63

 [21] Vyas NK., Vyas MN., Quiocho FA., 1991, Comparison of the periplasmic receptors for L-arabinose, D-glucose/D-galactose, and D-ribose. Structural and Functional Similarity., J Biol Chem 266(8):5226-37

 [22] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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