RegulonDB RegulonDB 10.7: Gene Form
   

motA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cheA motB motA flhC CpxR anti-anti-terminator anti-terminator terminator motAp motAp TSS_2231 TSS_2231 TSS_2230 TSS_2230 TSS_2229 TSS_2229 TSS_2228 TSS_2228 cheWp5 cheWp5

Gene      
Name: motA    Texpresso search in the literature
Synonym(s): ECK1891, EG10601, b1890, flaJ
Genome position(nucleotides): 1976252 <-- 1977139 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.35
Note(s): MotA and MotB are membrane embedded and do not fractionate
with the other hook-basal body proteins Ridgway HG, Silverman M, Simon MI,1977 In freeze-fracture electron microscopy, MotA and MotB can be visualized as circular
ring particles or studs lying in a plane with the inner membrane of Escherichia coli Khan S, Dapice M, Reese TS,1988 Both proteins span the cytoplasmic
membrane with MotA having four membrane-spanning alpha-helical segments and about two-thirds of its mass in the cytoplasm Blair DF, Berg HC,1991
MotB has one membrane-spanning alpha-helix but the majority of the molecule is in the periplasmic space with a peptidoglycan-binding domain near
its C terminus Chun SY, Parkinson JS,1988 MotB is thought to anchor MotA to the cell wall's rigid framework, allowing the MotA/MotB complex to serve as the
stationary stator of the flagellar motor Berg HC,2003 The MotA/MotB complex conducts protons across the membrane and then couples this
proton flow to rotational torque of the motor Kojima S, Blair DF,2004in a process that involves the protonation/deprotonation of the Asp32 residue of
MotB Braun TF, Blair DF,2001 Residues have also been defined in MotA's cytoplasmic domain which are critical for interaction with charged residues in
rotor protein FliG Zhou J, Blair DF,1997 The stoichiometric composition per motor has been determined as being MotA4 MotB2 Kojima S, Blair DF,2004
External database links:  
ASAP:
ABE-0006299
CGSC:
491
ECHOBASE:
EB0596
OU-MICROARRAY:
b1890
PORTECO:
motA
REGULONDB:
b1890
STRING:
511145.b1890
M3D: motA
COLOMBOS: motA
PortEco: b1890


Product      
Name: motility protein A
Synonym(s): FlaJ, MotA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 32.011
Isoelectric point: 5.385
Motif(s):
 
Type Positions Sequence
93 -> 93 G
139 -> 239 NTFEIEALMDEEIETHESEAEVPANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQ
201 -> 222 LIAHAMVGTFLGILLAYGFISP
2 -> 21 LILLGYLVVLGTVFGGYLMT
96 -> 96 S

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
  5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0120101 - bacterial-type flagellum stator complex
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0006811 - ion transport
GO:0006935 - chemotaxis
GO:0071973 - bacterial-type flagellum-dependent cell motility
GO:0071978 - bacterial-type flagellum-dependent swarming motility
GO:0097588 - archaeal or bacterial-type flagellum-dependent cell motility
Note(s): Note(s): ...[more].
Reference(s): [1] Minamino T., et al., 2018
[2] Onoue Y., et al., 2018
External database links:  
DIP:
DIP-10244N
ECOCYC:
MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN
ECOLIWIKI:
b1890
INTERPRO:
IPR000540
INTERPRO:
IPR022522
INTERPRO:
IPR002898
MINT:
MINT-1250843
PANTHER:
PTHR30433:SF4
PFAM:
PF01618
PRIDE:
P09348
PRODB:
PRO_000023274
PROSITE:
PS01307
PROTEINMODELPORTAL:
P09348
REFSEQ:
NP_416404
SMR:
P09348
UNIPROT:
P09348


Operon      
Name: motAB-cheAW         
Operon arrangement:
Transcription unit        Promoter
motAB-cheAW


Transcriptional Regulation      
Display Regulation             
Repressed by: CpxR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter cheWp5 1973462 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter TSS_2228 1973726 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2229 1975808 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2230 1976720 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2231 1977300 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Minamino T., Terahara N., Kojima S., Namba K., 2018, Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment., Mol Microbiol 109(6):723-734

 [2] Onoue Y., Takekawa N., Nishikino T., Kojima S., Homma M., 2018, The role of conserved charged residues in the bidirectional rotation of the bacterial flagellar motor., Microbiologyopen 7(4):e00587

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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