RegulonDB RegulonDB 10.6.3: Gene Form
   

mreB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

csrD mreC mreB yhdHp1 yhdHp1 TSS_3584 TSS_3584 mreBp3 mreBp3 mreBp2 mreBp2 TSS_3583 TSS_3583 mreBp1 mreBp1 TSS_3582 TSS_3582 TSS_3581 TSS_3581 TSS_3580 TSS_3580 TSS_3579 TSS_3579 mreDp2 mreDp2

Gene      
Name: mreB    Texpresso search in the literature
Synonym(s): ECK3239, EG10608, b3251, envB, mon, mreB_1, rodY
Genome position(nucleotides): 3400044 <-- 3401087 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.31
Reference(s): [1] Normark S. 1969
External database links:  
ASAP:
ABE-0010661
CGSC:
31343
ECHOBASE:
EB0603
ECOCYC:
EG10608
ECOLIHUB:
mreB
OU-MICROARRAY:
b3251
REGULONDB:
b3251
STRING:
511145.b3251
M3D: mreB
COLOMBOS: mreB
PortEco: b3251


Product      
Name: dynamic cytoskeletal protein MreB
Synonym(s): EnvB, Mon, MreB, MreB_1, RodY
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,cytoskeleton,inner membrane
Molecular weight: 36.952
Isoelectric point: 4.927
Motif(s):
 
Type Positions Sequence
11 -> 338 NDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDM
60 -> 61 KQ
161 -> 181 SMVVDIGGGTTEVAVISLNGV
21 -> 21 N
83 -> 83 D

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations
Gene Ontology Terms (GO)  
cellular_component GO:0099513 - polymeric cytoskeletal fiber
GO:0005737 - cytoplasm
GO:0005886 - plasma membrane
GO:0031226 - intrinsic component of plasma membrane
GO:0005856 - cytoskeleton
molecular_function GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0042802 - identical protein binding
biological_process GO:0000902 - cell morphogenesis
GO:0043093 - FtsZ-dependent cytokinesis
GO:0008360 - regulation of cell shape
GO:0051782 - negative regulation of cell division
GO:0051983 - regulation of chromosome segregation
Note(s): Note(s): ...[more].
Evidence: [AUP] Assay of unpurified protein
[AUPEINH] Assay of unpurified protein expressed in its native host
[IHBCE] Inferred by a human based on computational evidence
[IMP] Inferred from mutant phenotype
Reference(s): [2] Alyahya SA., et al., 2009
[3] Bendezu FO., et al., 2008
[4] Bork P., et al., 1992
[5] Fenton AK., et al., 2013
[6] Freire P., et al., 2009
[7] Iwai N., et al., 2002
[8] Kawazura T., et al., 2017
[9] Kruse T., et al., 2005
[10] Kruse T., et al., 2003
[11] Maeda YT., et al., 2012
[12] Monds RD., et al., 2014
[13] Morgenstein RM., et al., 2015
[14] Nilsen T., et al., 2005
[15] Ouzounov N., et al., 2016
[16] Saikia K., et al., 2017
[17] Shi H., et al., 2017
[18] Shih YL., et al., 2005
[19] Shiomi D., et al., 2013
[20] Swulius MT., et al., 2011
[21] Varma A., et al., 2009
[22] Vats P., et al., 2009
[23] Wachi M., et al., 1989
[24] Wachi M., et al., 1987
[25] Wang S., et al., 2010
[26] van den Ent F., et al., 2010
External database links:  
DIP:
DIP-31874N
ECOCYC:
EG10608-MONOMER
ECOLIWIKI:
b3251
INTERPRO:
IPR004753
MINT:
P0A9X4
MODBASE:
P0A9X4
PANTHER:
PTHR42749
PFAM:
PF06723
PRIDE:
P0A9X4
PRINTS:
PR01652
PRODB:
PRO_000023289
PROTEINMODELPORTAL:
P0A9X4
REFSEQ:
NP_417717
SMR:
P0A9X4
SWISSMODEL:
P0A9X4
UNIPROT:
P0A9X4


Operon      
Name: mreBCD         
Operon arrangement:
Transcription unit        Promoter
mreB
mreBCD
mreB
mreBCD
mreB
mreBCD
mreCD


Transcriptional Regulation      
Display Regulation             
Repressed by: BolA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mreDp2 3399083 reverse Similarity to the consensus
Read more >
[ICWHO] [27]
  promoter TSS_3579 3399805 reverse nd [RS-EPT-CBR] [28]
  promoter TSS_3580 3400666 reverse nd [RS-EPT-CBR] [28]
  promoter TSS_3581 3400674 reverse nd [RS-EPT-CBR] [28]
  promoter TSS_3582 3401076 reverse nd [RS-EPT-CBR] [28]
  promoter TSS_3583 3401192 reverse nd [RS-EPT-CBR] [28]
  promoter TSS_3584 3403230 forward nd [RS-EPT-CBR] [28]
  promoter yhdHp1 3403280 forward Similarity to the consensus
Read more >
[ICWHO] [27]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Normark S., 1969, Mutation in Escherichia coli K-12 mediating spherelike envelopes and changes tolerance to ultraviolet irradiation and some antibiotics., J Bacteriol 98(3):1274-7

 [2] Alyahya SA., Alexander R., Costa T., Henriques AO., Emonet T., Jacobs-Wagner C., 2009, RodZ, a component of the bacterial core morphogenic apparatus., Proc Natl Acad Sci U S A 106(4):1239-44

 [3] Bendezu FO., de Boer PA., 2008, Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli., J Bacteriol 190(5):1792-811

 [4] Bork P., Sander C., Valencia A., 1992, An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins., Proc Natl Acad Sci U S A 89(16):7290-4

 [5] Fenton AK., Gerdes K., 2013, Direct interaction of FtsZ and MreB is required for septum synthesis and cell division in Escherichia coli., EMBO J 32(13):1953-65

 [6] Freire P., Moreira RN., Arraiano CM., 2009, BolA inhibits cell elongation and regulates MreB expression levels., J Mol Biol 385(5):1345-51

 [7] Iwai N., Nagai K., Wachi M., 2002, Novel S-benzylisothiourea compound that induces spherical cells in Escherichia coli probably by acting on a rod-shape-determining protein(s) other than penicillin-binding protein 2., Biosci Biotechnol Biochem 66(12):2658-62

 [8] Kawazura T., Matsumoto K., Kojima K., Kato F., Kanai T., Niki H., Shiomi D., 2017, Exclusion of assembled MreB by anionic phospholipids at cell poles confers cell polarity for bidirectional growth., Mol Microbiol 104(3):472-486

 [9] Kruse T., Bork-Jensen J., Gerdes K., 2005, The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex., Mol Microbiol 55(1):78-89

 [10] Kruse T., Moller-Jensen J., Lobner-Olesen A., Gerdes K., 2003, Dysfunctional MreB inhibits chromosome segregation in Escherichia coli., EMBO J 22(19):5283-92

 [11] Maeda YT., Nakadai T., Shin J., Uryu K., Noireaux V., Libchaber A., 2012, Assembly of MreB filaments on liposome membranes: a synthetic biology approach., ACS Synth Biol 1(2):53-9

 [12] Monds RD., Lee TK., Colavin A., Ursell T., Quan S., Cooper TF., Huang KC., 2014, Systematic perturbation of cytoskeletal function reveals a linear scaling relationship between cell geometry and fitness., Cell Rep 9(4):1528-37

 [13] Morgenstein RM., Bratton BP., Nguyen JP., Ouzounov N., Shaevitz JW., Gitai Z., 2015, RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis., Proc Natl Acad Sci U S A 112(40):12510-5

 [14] Nilsen T., Yan AW., Gale G., Goldberg MB., 2005, Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB., J Bacteriol 187(17):6187-96

 [15] Ouzounov N., Nguyen JP., Bratton BP., Jacobowitz D., Gitai Z., Shaevitz JW., 2016, MreB Orientation Correlates with Cell Diameter in Escherichia coli., Biophys J 111(5):1035-43

 [16] Saikia K., Sravani YD., Ramakrishnan V., Chaudhary N., 2017, Highly potent antimicrobial peptides from N-terminal membrane-binding region of E. coli MreB., Sci Rep 7:42994

 [17] Shi H., Colavin A., Bigos M., Tropini C., Monds RD., Huang KC., 2017, Deep Phenotypic Mapping of Bacterial Cytoskeletal Mutants Reveals Physiological Robustness to Cell Size., Curr Biol 27(22):3419-3429.e4

 [18] Shih YL., Kawagishi I., Rothfield L., 2005, The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation., Mol Microbiol 58(4):917-28

 [19] Shiomi D., Toyoda A., Aizu T., Ejima F., Fujiyama A., Shini T., Kohara Y., Niki H., 2013, Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells., Mol Microbiol 87(5):1029-44

 [20] Swulius MT., Chen S., Jane Ding H., Li Z., Briegel A., Pilhofer M., Tocheva EI., Lybarger SR., Johnson TL., Sandkvist M., Jensen GJ., 2011, Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria., Biochem Biophys Res Commun 407(4):650-5

 [21] Varma A., Young KD., 2009, In Escherichia coli, MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2., J Bacteriol 191(11):3526-33

 [22] Vats P., Shih YL., Rothfield L., 2009, Assembly of the MreB-associated cytoskeletal ring of Escherichia coli., Mol Microbiol 72(1):170-82

 [23] Wachi M., Doi M., Okada Y., Matsuhashi M., 1989, New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells., J Bacteriol 171(12):6511-6

 [24] Wachi M., Doi M., Tamaki S., Park W., Nakajima-Iijima S., Matsuhashi M., 1987, Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in Escherichia coli., J Bacteriol 169(11):4935-40

 [25] Wang S., Arellano-Santoyo H., Combs PA., Shaevitz JW., 2010, Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria., Proc Natl Acad Sci U S A 107(20):9182-5

 [26] van den Ent F., Johnson CM., Persons L., de Boer P., Lowe J., 2010, Bacterial actin MreB assembles in complex with cell shape protein RodZ., EMBO J 29(6):1081-90

 [27] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [28] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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