RegulonDB

Gene
Name:	nupG
Synonym(s):	ECK2959, EG10664, b2964, gru
Genome position(nucleotides):	3105714 --> 3106970 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.72
External database links:

ASAP:	ABE-0009728
CGSC:	442
ECHOBASE:	EB0658
ECOLIHUB:	nupG
OU-MICROARRAY:	b2964
STRING:	511145.b2964
COLOMBOS:	nupG

Gene

Name:

nupG

Synonym(s):

ECK2959, EG10664, b2964, gru

Genome position(nucleotides):

3105714 --> 3106970 Genome Browser

Strand:

forward

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

49.72

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Product

Name:

nucleoside:H⁺ symporter NupG

Synonym(s):

Gru, NupG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

46.389

Isoelectric point:

8.799

Motif(s):

Type	Positions	Sequence
	1 -> 406	MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHMQLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMAMFKYKH
	40 -> 60	IGAVYSSLGIAAVFMPALLGI
	136 -> 136	R
	210 -> 230	MAIFFIFSMLLGAELQITNMF
	318 -> 318	Y

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015506 - nucleoside:proton symporter activity
	biological_process	GO:1901642 - nucleoside transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Doskocil J. 1974
[2] Jindal S., et al., 2019
[3] Komatsu Y. 1971
[4] Komatsu Y., et al., 1972
[5] Leung KK., et al., 1977
[6] Munch-Petersen A., et al., 1990
[7] Munch-Petersen A., et al., 1980
[8] Mygind B., et al., 1975
[9] Pao SS., et al., 1998
[10] Tong M., et al., 2020
[11] Vaziri H., et al., 2013

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	CRP, DeoR, CytR

Transcriptional Regulation

Display Regulation

Activated by:

CRP

Repressed by:

CRP, DeoR, CytR

RNA cis-regulatory element

Attenuation:	Transcriptional

RNA cis-regulatory element

Attenuation:

Transcriptional

Reference(s)
[1] Doskocil J., 1974, Inducible nucleoside permease in Escherichia coli., Biochem Biophys Res Commun 56(4):997-1003
[2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195
[3] Komatsu Y., 1971, Mechanism of action of showdomycin. V. Reduced ability of showdomycin-resistant mutants of Escherichia coli K-12 to take up showdomycin and nucleosides., J Antibiot (Tokyo) 24(12):876-83
[4] Komatsu Y., Tanaka K., 1972, A showdomycin-resistant mutant of Escherichia coli K-12 with altered nucleoside transport character., Biochim Biophys Acta 288(2):390-403
[5] Leung KK., Visser DW., 1977, Uridine and cytidine transport in Escherichia coli B and transport-deficient mutants., J Biol Chem 252(8):2492-7
[6] Munch-Petersen A., Jensen N., 1990, Analysis of the regulatory region of the Escherichia coli nupG gene, encoding a nucleoside-transport protein., Eur J Biochem 190(3):547-51
[7] Munch-Petersen A., Pihl NJ., 1980, Stimulatory effect of low ATP pools on transport of purine nucleosides in cells of Escherichia coli., Proc Natl Acad Sci U S A 77(5):2519-23
[8] Mygind B., Munch-Petersen., 1975, Transport of pyrimidine nucleosides in cells of Escherichia coli K 12., Eur J Biochem 59(2):365-72
[9] Pao SS., Paulsen IT., Saier MH., 1998, Major facilitator superfamily., Microbiol Mol Biol Rev 62(1):1-34
[10] Tong M., French S., El Zahed SS., Ong WK., Karp PD., Brown ED., 2020, Gene Dispensability in Escherichia coli Grown in Thirty Different Carbon Environments., mBio 11(5)
[11] Vaziri H., Baldwin SA., Baldwin JM., Adams DG., Young JD., Postis VL., 2013, Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG., Mol Membr Biol 30(2):114-28

Reference(s)

[1] Doskocil J., 1974, Inducible nucleoside permease in Escherichia coli., Biochem Biophys Res Commun 56(4):997-1003

[2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

[3] Komatsu Y., 1971, Mechanism of action of showdomycin. V. Reduced ability of showdomycin-resistant mutants of Escherichia coli K-12 to take up showdomycin and nucleosides., J Antibiot (Tokyo) 24(12):876-83

[4] Komatsu Y., Tanaka K., 1972, A showdomycin-resistant mutant of Escherichia coli K-12 with altered nucleoside transport character., Biochim Biophys Acta 288(2):390-403

[5] Leung KK., Visser DW., 1977, Uridine and cytidine transport in Escherichia coli B and transport-deficient mutants., J Biol Chem 252(8):2492-7

[6] Munch-Petersen A., Jensen N., 1990, Analysis of the regulatory region of the Escherichia coli nupG gene, encoding a nucleoside-transport protein., Eur J Biochem 190(3):547-51

[7] Munch-Petersen A., Pihl NJ., 1980, Stimulatory effect of low ATP pools on transport of purine nucleosides in cells of Escherichia coli., Proc Natl Acad Sci U S A 77(5):2519-23

[8] Mygind B., Munch-Petersen., 1975, Transport of pyrimidine nucleosides in cells of Escherichia coli K 12., Eur J Biochem 59(2):365-72

[9] Pao SS., Paulsen IT., Saier MH., 1998, Major facilitator superfamily., Microbiol Mol Biol Rev 62(1):1-34

[10] Tong M., French S., El Zahed SS., Ong WK., Karp PD., Brown ED., 2020, Gene Dispensability in Escherichia coli Grown in Thirty Different Carbon Environments., mBio 11(5)

[11] Vaziri H., Baldwin SA., Baldwin JM., Adams DG., Young JD., Postis VL., 2013, Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG., Mol Membr Biol 30(2):114-28