RegulonDB RegulonDB 10.8: Gene Form
   

ompT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ompT envY appY PhoP OmrB OmrA ompTp ompTp TSS_695 (cluster) TSS_695 (cluster) TSS_694 TSS_694 TSS_693 TSS_693 TSS_692 TSS_692 TSS_691 TSS_691 TSS_690 (cluster) TSS_690 (cluster) TSS_689 TSS_689 TSS_688 TSS_688 TSS_687 TSS_687

Gene      
Name: ompT    Texpresso search in the literature
Synonym(s): ECK0557, EG10673, b0565
Genome position(nucleotides): 584680 <-- 585633 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
43.4
External database links:  
ASAP:
ABE-0001931
CGSC:
4984
ECHOBASE:
EB0667
OU-MICROARRAY:
b0565
PortEco:
ompT
STRING:
511145.b0565
COLOMBOS: ompT


Product      
Name: DLP12 prophage; protease 7
Synonym(s): OmpT, omptin, outer membrane protease VII (outer membrane protein 3b)
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 35.562
Isoelectric point: 5.83
Motif(s):
 
Type Positions Sequence
238 -> 238 R
265 -> 274 AKVYVEGAWN
21 -> 317 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF
105 -> 105 D
28 -> 317 TPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides
  6 - cell structure --> 6.1 - membrane
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0016021 - integral component of membrane
GO:0031230 - intrinsic component of cell outer membrane
molecular_function GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0004175 - endopeptidase activity
GO:0004190 - aspartic-type endopeptidase activity
GO:0004252 - serine-type endopeptidase activity
biological_process GO:0006508 - proteolysis
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Hamdallah I., et al., 2018
[2] Kukkonen M., et al., 2004
[3] Stathopoulos C. 1998
[4] Zhang DF., et al., 2018
External database links:  
ECOCYC:
EG10673-MONOMER
ECOLIWIKI:
b0565
INTERPRO:
IPR020079
INTERPRO:
IPR020080
INTERPRO:
IPR000036
MODBASE:
P09169
PDB:
1I78
PFAM:
PF01278
PRIDE:
P09169
PRINTS:
PR00482
PRODB:
PRO_000023458
PROSITE:
PS00835
PROSITE:
PS00834
REFSEQ:
NP_415097
SMR:
P09169
UNIPROT:
P09169


Operon      
Name: envY-ompT         
Operon arrangement:
Transcription unit        Promoter
ompT
envY-ompT


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP


Regulation by small RNA    
  Display Regulation
small RNA omrA, omrB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_687 584165 forward nd [RS-EPT-CBR] [5]
  promoter TSS_688 585378 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_689 585380 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_690 (cluster) 585383 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [5]
  promoter TSS_691 585398 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_692 585408 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_693 585420 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_694 585594 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_695 (cluster) 585659 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Hamdallah I., Torok N., Bischof KM., Majdalani N., Chadalavada S., Mdluli N., Creamer KE., Clark M., Holdener C., Basting PJ., Gottesman S., Slonczewski JL., 2018, Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction., Appl Environ Microbiol 84(15)

 [2] Kukkonen M., Korhonen TK., 2004, The omptin family of enterobacterial surface proteases/adhesins: from housekeeping in Escherichia coli to systemic spread of Yersinia pestis., Int J Med Microbiol 294(1):7-14

 [3] Stathopoulos C., 1998, Structural features, physiological roles, and biotechnological applications of the membrane proteases of the OmpT bacterial endopeptidase family: a micro-review., Membr Cell Biol 12(1):1-8

 [4] Zhang DF., Ye JZ., Dai HH., Lin XM., Li H., Peng XX., 2018, Identification of ethanol tolerant outer membrane proteome reveals OmpC-dependent mechanism in a manner of EnvZ/OmpR regulation in Escherichia coli., J Proteomics 179:92-99

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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