RegulonDB RegulonDB 11.0: Gene Form
   

pcnB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pcnB gluQ yadN folK DksA-ppGpp DksA-ppGpp DksA-ppGpp DksA-ppGpp ppGpp ppGpp pcnBp4 pcnBp4 pcnBp2 pcnBp2 pcnBp3 pcnBp3 pcnBp pcnBp pcnBp1 pcnBp1 yadNp7 yadNp7 yadNp8 yadNp8 yadNp1 yadNp1

Gene      
Name: pcnB    Texpresso search in the literature
Synonym(s): ECK0142, EG10690, b0143
Genome position(nucleotides): 157729 <-- 159126
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.87
External database links:  
ASAP:
 ABE-0000493
CGSC:
 13584
ECHOBASE:
 EB0684
ECOLIHUB:
 pcnB
MIM:
 616084
MIM:
 616959
OU-MICROARRAY:
 b0143
STRING:
 511145.b0143
COLOMBOS: pcnB


Product      
Name: poly(A) polymerase I
Synonym(s): PAP I, PcnB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 53.871
Isoelectric point: 10.116
Motif(s):
 
Type Positions Sequence Comment
1 -> 10 MFTRVANFCR
11 -> 465 KVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRTRRPRKRAPRREGTA UniProt: Poly(A) polymerase I.
62 -> 193 AWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIR
93 -> 93 R UniProt: In Ref. 1; AAA24303..
220 -> 281 GMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITR

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0016779 - nucleotidyltransferase activity
GO:0016740 - transferase activity
GO:0003723 - RNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004652 - polynucleotide adenylyltransferase activity
biological_process GO:0006276 - plasmid maintenance
GO:0009451 - RNA modification
GO:0006396 - RNA processing
GO:0006397 - mRNA processing
GO:0006378 - mRNA polyadenylation
GO:0043631 - RNA polyadenylation
Note(s): Note(s): ...[more].
Reference(s): [1] Cho HD., et al., 2007
[2] Just A., et al., 2008
[3] Maes A., et al., 2017
External database links:  
ALPHAFOLD:
 P0ABF1
DIP:
 DIP-47859N
ECOCYC:
 EG10690-MONOMER
ECOLIWIKI:
 b0143
INTERPRO:
 IPR002646
INTERPRO:
 IPR025866
INTERPRO:
 IPR043519
INTERPRO:
 IPR032828
INTERPRO:
 IPR010206
MODBASE:
 P0ABF1
PFAM:
 PF12626
PFAM:
 PF01743
PFAM:
 PF12627
PRIDE:
 P0ABF1
PRODB:
 PRO_000023500
REFSEQ:
 NP_414685
SMR:
 P0ABF1
UNIPROT:
 P0ABF1


Operon      
Name: pcnB-folK         
Operon arrangement:
Transcription unit        Promoter
pcnB-folK
pcnB-folK
pcnB-folK
pcnB-folK
pcnB-folk


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yadNp1 156984 reverse nd [ICWHO] [4]
  promoter yadNp8 157031 reverse nd [ICWHO] [4]
  promoter yadNp7 157055 reverse nd [ICWHO] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Cho HD., Verlinde CL., Weiner AM., 2007, Reengineering CCA-adding enzymes to function as (U,G)- or dCdCdA-adding enzymes or poly(C,A) and poly(U,G) polymerases., Proc Natl Acad Sci U S A 104(1):54-9

 [2] Just A., Butter F., Trenkmann M., Heitkam T., Morl M., Betat H., 2008, A comparative analysis of two conserved motifs in bacterial poly(A) polymerase and CCA-adding enzyme., Nucleic Acids Res 36(16):5212-20

 [3] Maes A., Gracia C., Innocenti N., Zhang K., Aurell E., Hajnsdorf E., 2017, Landscape of RNA polyadenylation in E. coli., Nucleic Acids Res 45(5):2746-2756

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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