RegulonDB RegulonDB 10.9: Gene Form
   

pgk gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pgk fbaA epd anti-anti-terminator anti-terminator terminator TSS_3260 TSS_3260 TSS_3259 (cluster) TSS_3259 (cluster) TSS_3258 TSS_3258 TSS_3257 (cluster) TSS_3257 (cluster) TSS_3256 (cluster) TSS_3256 (cluster) pgkp1 pgkp1 TSS_3255 TSS_3255 TSS_3254 TSS_3254 pgkp3 pgkp3 TSS_3252 (cluster) TSS_3252 (cluster) pgkp2 pgkp2 TSS_3250 (cluster) TSS_3250 (cluster) TSS_3249 (cluster) TSS_3249 (cluster) TSS_3248 TSS_3248 TSS_3247 TSS_3247 TSS_3246 TSS_3246 TSS_3245 (cluster) TSS_3245 (cluster) TSS_3244 (cluster) TSS_3244 (cluster) TSS_3243 TSS_3243 TSS_3242 TSS_3242 TSS_3241 TSS_3241 TSS_3240 TSS_3240 TSS_3239 TSS_3239 TSS_3238 TSS_3238 TSS_3237 TSS_3237 TSS_3236 TSS_3236 TSS_3235 TSS_3235 TSS_3234 TSS_3234 TSS_3233 TSS_3233 TSS_3232 (cluster) TSS_3232 (cluster) TSS_3231 TSS_3231 TSS_3230 TSS_3230 TSS_3229 TSS_3229 TSS_3228 TSS_3228 TSS_3227 (cluster) TSS_3227 (cluster) TSS_3226 TSS_3226 TSS_3225 TSS_3225 TSS_3224 TSS_3224 TSS_3223 TSS_3223 TSS_3222 TSS_3222 TSS_3221 TSS_3221 TSS_3220 TSS_3220 TSS_3219 (cluster) TSS_3219 (cluster) TSS_3218 (cluster) TSS_3218 (cluster) TSS_3217 TSS_3217 TSS_3216 TSS_3216 TSS_3215 TSS_3215 TSS_3214 TSS_3214 TSS_3213 TSS_3213 TSS_3212 TSS_3212 TSS_3211 TSS_3211 TSS_3210 TSS_3210 TSS_3209 TSS_3209

Gene      
Name: pgk    Texpresso search in the literature
Synonym(s): ECK2922, EG10703, b2926
Genome position(nucleotides): 3071459 <-- 3072622 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.32
Reference(s): [1] Alefounder PR., et al., 1989
External database links:  
ASAP:
ABE-0009605
CGSC:
408
ECHOBASE:
EB0697
ECOLIHUB:
pgk
MIM:
300653
OU-MICROARRAY:
b2926
STRING:
511145.b2926
COLOMBOS: pgk


Product      
Name: phosphoglycerate kinase
Synonym(s): Pgk
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 41.118
Isoelectric point: 4.838
Motif(s):
 
Type Positions Sequence
59 -> 62 HLGR
340 -> 343 GGDT
21 -> 23 DLN
2 -> 387 SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK
6 -> 373 MTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.1 - glycolysis
  1 - metabolism --> 1.7 - central intermediary metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004618 - phosphoglycerate kinase activity
GO:0043531 - ADP binding
biological_process GO:1903862 - positive regulation of oxidative phosphorylation
GO:0006094 - gluconeogenesis
GO:0006096 - glycolytic process
GO:0016310 - phosphorylation
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-36163N
ECOCYC:
PGK
ECOLIWIKI:
b2926
INTERPRO:
IPR036043
INTERPRO:
IPR015911
INTERPRO:
IPR001576
INTERPRO:
IPR015824
PANTHER:
PTHR11406
PDB:
1ZMR
PFAM:
PF00162
PRIDE:
P0A799
PRINTS:
PR00477
PRODB:
PRO_000023523
PROSITE:
PS00111
REFSEQ:
NP_417401
SMR:
P0A799
SWISSMODEL:
P0A799
UNIPROT:
P0A799


Operon      
Name: epd-pgk-fbaA         
Operon arrangement:
Transcription unit        Promoter
pgk
pgk-fbaA
pgk
pgk-fbaA
pgk
pgk-fbaA
epd-pgk
epd-pgk-fbaA
epd-pgk
epd-pgk-fbaA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3209 3070765 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3210 3070776 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3211 3070817 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3212 3070829 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3213 3070877 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3214 3070946 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3215 3070948 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3216 3070980 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3217 3071020 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3218 (cluster) 3071228 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3219 (cluster) 3071234 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3220 3071237 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3221 3071240 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3222 3071246 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3223 3071253 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3224 3071257 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3225 3071259 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3226 3071272 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3227 (cluster) 3071402 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3228 3071654 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3229 3071673 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3230 3071681 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3231 3071926 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3232 (cluster) 3071970 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3233 3071974 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3234 3072004 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3235 3072006 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3236 3072009 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3237 3072011 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3238 3072030 forward nd [RS-EPT-CBR] [2]
  promoter TSS_3239 3072032 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3240 3072041 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3241 3072121 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3242 3072341 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3243 3072348 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3244 (cluster) 3072367 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3245 (cluster) 3072371 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3246 3072373 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3247 3072375 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3248 3072393 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3249 (cluster) 3072629 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3250 (cluster) 3072642 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3252 (cluster) 3072682 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3254 3072831 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3255 3072852 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3256 (cluster) 3072860 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3257 (cluster) 3072929 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3258 3072931 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3259 (cluster) 3072934 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3260 3073321 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Alefounder PR., Perham RN., 1989, Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli., Mol Microbiol 3(6):723-32

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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