RegulonDB RegulonDB 9.1: Gene Form
   

ppc gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ppc eptC argE Cra terminator TSS_4683 TSS_4683 TSS_4682 TSS_4682 TSS_4681 TSS_4681 TSS_4680 TSS_4680 ppcp ppcp TSS_4679 (cluster) TSS_4679 (cluster) TSS_4678 TSS_4678 TSS_4677 TSS_4677 TSS_4676 (cluster) TSS_4676 (cluster) TSS_4675 TSS_4675 TSS_4674 TSS_4674 TSS_4673 TSS_4673 TSS_4672 TSS_4672 TSS_4671 TSS_4671 TSS_4670 TSS_4670 TSS_4669 TSS_4669 TSS_4668 TSS_4668 TSS_4667 TSS_4667 TSS_4666 TSS_4666 TSS_4665 TSS_4665 TSS_4664 (cluster) TSS_4664 (cluster) TSS_4663 (cluster) TSS_4663 (cluster) TSS_4662 TSS_4662 TSS_4661 TSS_4661 TSS_4660 TSS_4660 TSS_4659 TSS_4659 TSS_4658 TSS_4658 TSS_4657 TSS_4657 TSS_4656 TSS_4656 TSS_4655 (cluster) TSS_4655 (cluster) TSS_4654 TSS_4654 yijPp9 yijPp9

Gene      
Name: ppc    Texpresso search in the literature
Synonym(s): asp, b3956, glu
Genome position(nucleotides): 4148470 <-- 4151121 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.66
External database links:  
ECOCYC:
EG10756
GENBANK:
1790393
GENBANK_RefSeq:
16131794
OU-MICROARRAY:
b3956
M3D: ppc
COLOMBOS: ppc
PortEco: b3956


Product      
Name: Ppc
Synonym(s): Asp, Glu
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 99.062
Isoelectric point: 5.4
Motif(s):
 
Type Positions Sequence
129 -> 883 LSLELVLTAHPTEITRRTLIHKMVEVNACLKQLDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVPNYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKATDLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARLKGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVRIDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAETREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETLDDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQKVVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKELRNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKEGQEPDPRVEQALMVTIAGIAAGMRNTG
587 -> 587 R
579 -> 579 H
138 -> 138 H

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0008964 - phosphoenolpyruvate carboxylase activity
GO:0000287 - magnesium ion binding
biological_process GO:0008152 - metabolic process
GO:0006099 - tricarboxylic acid cycle
GO:0015977 - carbon fixation
GO:0006107 - oxaloacetate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Canovas JL., et al., 1965
[2] Coomes MW., et al., 1985
[3] Inoue M., et al., 1989
[4] Ishijima S., et al., 1986
[5] Kodaki T., et al., 1984
[6] Kwon YD., et al., 2008
[7] Sabe H., et al., 1984
[8] Tan Z., et al., 2013
[9] Terada K., et al., 1991
[10] Vandedrinck S., et al., 2001
External database links:  
ECOCYC:
PEPCARBOX-MONOMER
EXPASY:
EC-4.1.1.31
GENPROTEC:
b3956
PDB:
1QB4
PDB:
1JQN
PDB:
1FIY


Operon      
Name: ppc         
Operon arrangement:
Transcription unit        Promoter
ppc


Transcriptional Regulation      
Display Regulation             
Note: The TF binding sites with unknown position are not displayed in the graphic.
Repressed by: Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yijPp9 4148334 reverse Similarity to the consensus
Read more >
[ICWHO] [11]
  promoter TSS_4654 4149091 reverse nd nd nd
  promoter TSS_4655 (cluster) 4149702 reverse For this promoter, there
Read more >
nd nd
  promoter TSS_4656 4149707 reverse nd nd nd
  promoter TSS_4657 4149709 reverse nd nd nd
  promoter TSS_4658 4149717 reverse nd nd nd
  promoter TSS_4659 4149721 reverse nd nd nd
  promoter TSS_4660 4149740 reverse nd nd nd
  promoter TSS_4661 4149917 reverse nd nd nd
  promoter TSS_4662 4149926 reverse nd nd nd
  promoter TSS_4663 (cluster) 4149941 reverse For this promoter, there
Read more >
nd nd
  promoter TSS_4664 (cluster) 4149945 reverse For this promoter, there
Read more >
nd nd
  promoter TSS_4665 4150069 reverse nd nd nd
  promoter TSS_4666 4150072 reverse nd nd nd
  promoter TSS_4667 4150076 reverse nd nd nd
  promoter TSS_4668 4150083 reverse nd nd nd
  promoter TSS_4669 4150108 reverse nd nd nd
  promoter TSS_4670 4150172 reverse nd nd nd
  promoter TSS_4671 4150176 reverse nd nd nd
  promoter TSS_4672 4150181 reverse nd nd nd
  promoter TSS_4673 4150187 reverse nd nd nd
  promoter TSS_4674 4150466 reverse nd nd nd
  promoter TSS_4675 4150551 reverse nd nd nd
  promoter TSS_4676 (cluster) 4150699 reverse For this promoter, there
Read more >
nd nd
  promoter TSS_4677 4150937 reverse nd nd nd
  promoter TSS_4678 4151144 reverse nd nd nd
  promoter TSS_4679 (cluster) 4151211 reverse For this promoter, there
Read more >
nd nd
  promoter TSS_4680 4151595 reverse nd nd nd
  promoter TSS_4681 4151598 reverse nd nd nd
  promoter TSS_4682 4151717 reverse nd nd nd
  promoter TSS_4683 4151720 reverse nd nd nd


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Canovas JL., Kornberg HL., 1965, Fine control of phosphopyruvate carboxylase activity in Escherichia coli., Biochim Biophys Acta. 96:169-72

 [2] Coomes MW., Mitchell BK., Beezley A., Smith TE., 1985, Properties of an Escherichia coli mutant deficient in phosphoenolpyruvate carboxylase catalytic activity., J Bacteriol. 164(2):646-52

 [3] Inoue M., Hayashi M., Sugimoto M., Harada S., Kai Y., Kasai N., Terada K., Izui K., 1989, First crystallization of a phosphoenolpyruvate carboxylase from Escherichia coli., J Mol Biol. 208(3):509-10

 [4] Ishijima S., Izui K., Katsuki H., 1986, Phosphoenolpyruvate carboxylase of Escherichia coli K-12. N- and C-terminal sequences and tentative assignment of the catalytically essential cysteine residue., J Biochem. 99(5):1299-310

 [5] Kodaki T., Fujita N., Kameshita I., Izui K., Katsuki H., 1984, Phosphoenolpyruvate carboxylase of Escherichia coli. Specificity of some compounds as activators at the site for fructose 1,6-bisphosphate, one of the allosteric effectors., J Biochem. 95(3):637-42

 [6] Kwon YD., Lee SY., Kim P., 2008, A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase., Biosci Biotechnol Biochem. 72(4):1138-41

 [7] Sabe H., Miwa T., Kodaki T., Izui K., Hiraga S., Katsuki H., 1984, Molecular cloning of the phosphoenolpyruvate carboxylase gene, ppc, of Escherichia coli., Gene. 31(1-3):279-83

 [8] Tan Z., Zhu X., Chen J., Li Q., Zhang X., 2013, Activating phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in combination for improvement of succinate production., Appl Environ Microbiol. 79(16):4838-44

 [9] Terada K., Murata T., Izui K., 1991, Site-directed mutagenesis of phosphoenolpyruvate carboxylase from E. coli: the role of His579 in the catalytic and regulatory functions., J Biochem (Tokyo). 109(1):49-54

 [10] Vandedrinck S., Deschamps G., Sablon E., Vandamme EJ., 2001, Construction and characterization of a PPC (phosphoenolpyruvate carboxylase) knockout mutant of Escherichia coli., Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 66(4):345-9

 [11] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol. 333(2):261-78


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