Gene
Name:	prfA
Synonym(s):	ECK1199, EG10761, b1211, sueB, uar, ups?
Genome position(nucleotides):	1265012 --> 1266094 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.0
External database links:

ASAP:	ABE-0004066
CGSC:	14922
ECHOBASE:	EB0754
OU-MICROARRAY:	b1211
PortEco:	prfA
STRING:	511145.b1211
COLOMBOS:	prfA

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1543 (cluster)	1264894	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1264894 to 1264895, where the highest frequency is at position 1264118. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_1544 (cluster)	1264899	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1264899 to 1264900, where the highest frequency is at position 1264123. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_1545	1265281	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_1546	1265487	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_1547 (cluster)	1267181	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1267181 to 1267182, where the highest frequency is at position 1266405. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_1548	1267190	forward	nd	[RS-EPT-CBR]	[8]

Reference(s)
[1] Baggett NE., Zhang Y., Gross CA., 2017, Global analysis of translation termination in E. coli., PLoS Genet 13(3):e1006676
[2] Florin T., Maracci C., Graf M., Karki P., Klepacki D., Berninghausen O., Beckmann R., Vazquez-Laslop N., Wilson DN., Rodnina MV., Mankin AS., 2017, An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome., Nat Struct Mol Biol 24(9):752-757
[3] Lajoie MJ., Rovner AJ., Goodman DB., Aerni HR., Haimovich AD., Kuznetsov G., Mercer JA., Wang HH., Carr PA., Mosberg JA., Rohland N., Schultz PG., Jacobson JM., Rinehart J., Church GM., Isaacs FJ., 2013, Genomically recoded organisms expand biological functions., Science 342(6156):357-60
[4] Mora L., Heurgue-Hamard V., de Zamaroczy M., Kervestin S., Buckingham RH., 2007, Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo., J Biol Chem 282(49):35638-45
[5] Scolnick E., Tompkins R., Caskey T., Nirenberg M., 1968, Release factors differing in specificity for terminator codons., Proc Natl Acad Sci U S A 61(2):768-74
[6] Shaw JJ., Green R., 2007, Two distinct components of release factor function uncovered by nucleophile partitioning analysis., Mol Cell 28(3):458-67
[7] Wei Y., Wang J., Xia X., 2016, Coevolution between Stop Codon Usage and Release Factors in Bacterial Species., Mol Biol Evol 33(9):2357-67
[8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Transcriptional Regulation
	Display Regulation
Activated by:	IHF, ArcA
Repressed by:	FNR